WorldCat Identities

Górecki, Marian

Overview
Works: 7 works in 9 publications in 3 languages and 11 library holdings
Genres: Terminology 
Roles: Author
Publication Timeline
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Most widely held works by Marian Górecki
O terminologii fizycznej by Marian Górecki( Book )

2 editions published in 1990 in Polish and held by 4 WorldCat member libraries worldwide

Production of Enzymatically Active Human Acetylcholinesterase in E. Coli( Book )

2 editions published between 1992 and 1993 in English and held by 2 WorldCat member libraries worldwide

Human acetylcholinesterase (hAChE) was expressed in Escherichia coli under regulation of the constitutive deo promoter or the thermoinducible lambda P sub L promoter. To facilitate the expression in the prokaryotic system, the recombinant human AChE (rhAChE) cDNA was modified at the N-terminus, by site- directed mutagenesis, in order to replace some of the guanisine and cytosine (GC)-rich regions by adenine thimidine (AT). These modifications did not alter the amino acid sequence but resulted in ample production of the protein. rhAChE accumulated in the cells and reached a level of 10% of total bacterial proteins. A partially purified inactive recombinant protein was recovered from inclusion bodies. Active rhAChE was obtained after solubilization, folding and oxidation; however, the overall yield of the active enzyme was low. A 20- to 40-fold increase in the process yield of active rhAChE activity was achieved by replacing Cys(580) by Ser. Substrate specificity and inhibitor selectivity of the recombinant mutant enzyme harboring Ser at amino acid number 580 were indistinguishable from those of the native AChE isolated from human erythrocytes
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1 edition published in 1971 in Hebrew and held by 1 WorldCat member library worldwide

Strategia zwycięstwa : szkice polityczne by Marian Górecki( Book )

1 edition published in 1944 in Polish and held by 1 WorldCat member library worldwide

Numerical analysis of stresses in mould in the process of pressure casting( )

1 edition published in 2013 in English and held by 1 WorldCat member library worldwide

O terminologii fizycznej by Marian Górecki( Book )

1 edition published in 1990 in Polish and held by 1 WorldCat member library worldwide

O terminologii fizycznej by Marian Górecki( Book )

1 edition published in 1990 in Polish and held by 1 WorldCat member library worldwide

 
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Audience Level
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  Kids General Special  
Audience level: 0.92 (from 0.52 for <> ... to 0.99 for Production ...)

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