WorldCat Identities

Moerner, W. E. (William Esco) 1953-

Works: 74 works in 98 publications in 2 languages and 325 library holdings
Genres: Conference papers and proceedings 
Roles: Author, Thesis advisor, Editor, Other
Classifications: QC176.8.O58, 543.0858
Publication Timeline
Most widely held works about W. E Moerner
Most widely held works by W. E Moerner
Persistent spectral hole-burning : science and application by W. E Moerner( Book )

11 editions published in 1988 in English and German and held by 190 WorldCat member libraries worldwide

This book describes the underlying scientific fundamentals and principal phenomena associated with persistent spectral hole-burning in solids, and presents an overview of possible future applications to optical storage of digital data and optical signal processing. Organization of the material is by the general physical mechanism responsible for the formation of persistent spectral holes. After a description of the basic principles and methods of hole-burning, with examples from photochemical processes in crystalline and amorphous hosts, the unusual proton tunneling phenomena that occur in hydrogen-bonded polymers and glasses are described. Persistent spectral hole-burning in inorganic materials due either to photoionization or to photophysical effects is then summarized, followed by a detailed discussion of nonphotochemical hole-burning mechanisms for electronic transitions in amorphous solids. The book concludes with a description of potential applications to data storage and optical processsing using frequency-domain, holographic, and electric field techniques. Readers of this volume will gain a detailed appreciation of both the generality of the persistent spectral hole-burning phenomenon and the power of the technique in studying microscopic dynamics and mechanisms of phototransformation in low-temperature solids
Persistent spectral hole burning( Book )

3 editions published between 1989 and 1992 in English and held by 5 WorldCat member libraries worldwide

This article reviews recent progress in the area of photon gated persistent spectral hole burning, in which one photon selects absorbers in an inhomogeneously broadened line and a second gating photon of a different wavelength completes the excitation necessary to produce a spectral hole. This phenomenon provides a crucial threshold in the hole formation process, allowing reading with the first wavelength alone to be nondestructive. Examples of photo gating in both inorganic and organic materials are summarized, with emphasis of the organic materials. Keywords: Photo gating; Persistent spectral hole burning; Frequency domain optical storage
Vibrational relaxation dynamics of an IR-laser-excited molecular impurity mode in alkali halide lattices by W. E Moerner( )

2 editions published in 1982 in English and held by 3 WorldCat member libraries worldwide

Statistical Fine Structure of Inhomogeneously Broadened Absorption Lines by W. E Moerner( Book )

3 editions published in 1987 in English and held by 3 WorldCat member libraries worldwide

Using laser frequency-modulation spectroscopy; we have observed statistical fine structure (SFS) in the inhomogeneously broadened optical absorption of pentacene in p-terphenyl at liquid helium temperatures. SFS is the actual frequency-dependent, time-independent structure of the inhomogeneous line caused by the randomly varying number of centers in each frequency interval. The size of the SFS varies as the square root of the number of centers, and the autocorrelation of the SFS yields and estimate of the homogeneous linewidth without requiring spectral hole-burning or coherent transients. Keywords: Spectroscopy of defects in solids; Molecular spectroscopy; Statistical effects
Observing protein dynamics and conformational changes by ensemble and single-molecule fluorescence spectroscopy by So Yeon Kim( Book )

1 edition published in 2008 in English and held by 2 WorldCat member libraries worldwide

Single-molecule and super-resolution in living cells by Hsiao-lu Lee( )

1 edition published in 2011 in English and held by 2 WorldCat member libraries worldwide

Since the first successful detection single molecules over two decades ago, single-molecule spectroscopy has developed into a burgeoning field with a wealth of experiments at room temperature and inside living cells. Probing asynchronous and heterogeneous populations in situ, one molecule at a time, is not only desirable, but critical for many biological questions. Further, super-resolution imaging based on sequential imaging of sparse subsets of single molecules, has seen explosive growth within the last five years. This dissertation describes both the application of live-cell single-molecule imaging as an answer to important biological questions, and development and validation of fluorescent probes for targeted super-resolution imaging
The development of techniques for three-dimensional super-resolution fluorescence microscopy and their application to biological systems by Michael Anthony Thompson( )

1 edition published in 2011 in English and held by 2 WorldCat member libraries worldwide

Fluorescence microscopy is one of the most widely used tools in cell biology due its intrinsically high detection sensitivity coupled with the ability to genetically label proteins and other cellular structures with fluorescent tags. However, the resolution of fluorescence microscopy has historically been limited to about 200 nm laterally and 800 nm axially because of the diffraction limit of visible light. In the past five years, imaging below the diffraction limit ("super-resolution imaging") by localizing single fluorophores, one at a time (1-3), has opened a wide a variety of new biological systems for study. This Dissertation is a collection of both techniques for two and three dimensional super-resolution imaging as well as applications in bacterial and yeast imaging. References 1. Betzig E, et al (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642-1645. 2. Hess ST, Girirajan TPK & Mason MD (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91: 4258-4272. 3. Rust MJ, Bates M & Zhuang X (2006) Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3: 793-795
Statistical fine structure in the inhomogeneously broadened electronic origin of pentacene in [rho sign]-terphenyl by T. P Carter( Book )

2 editions published in 1988 in English and held by 2 WorldCat member libraries worldwide

Recently, the first observation of statistical fine structure on an inhomogeneously broadened absorption profile was reported (W.E. Moerner, T.P. Carter, Phys. Rev. Lett. 59,2705(1987) for mixed crystals of pentacene in p-terphenyl using laser FM spectroscopy. Statistical fine structure is time-independent structure on the inhomogeneous line caused by statistical variations, in the spectral density of absorbers in each frequency interval. In this work, a model and an analysis of statistical fine structure using autocorrelation techniques are presented, and the dependence of the effect for pentacene in p-terphenyl at 1.4K on modulating frequency, detection phase, sample position, center concentration, and site is described. Keywords: Statistical fine structure, Inhomogeneous broadening, Molecular spectroscopy, Defects, Solids
Finding a Single Molecule in a Haystack: Optical Detection and Spectroscopy of Single Absorbers in Solids( Book )

2 editions published in 1989 in English and held by 2 WorldCat member libraries worldwide

Using two different double modulation techniques, we have observed the optical absorption spectrum of single dopant molecules of pentacene in a p-terphenyl host crystal at liquid helium temperatures. To achieve this frequency-modulation spectroscopy was combined either with Stark or ultrasonic modulation to remove interfering background signals from residual amplitude modulation, and the number of molecules in resonance was reduced to one by operating in the wings of the inhomogeneous line. Triplet bottleneck saturation appears in the single-molecule regime. Keywords: Laser spectroscopy; Impurity absorption; Ultrasensitive detection; Single absorber effects. (MJM)
Molecular characterization of cell division machinery in caulobacter crescentus by Yi Chun Yeh( )

1 edition published in 2010 in English and held by 2 WorldCat member libraries worldwide

Cell division is a major developmental event in the life cycle of a bacterial cell. Caulobacter crescentus division is asymmetric, producing daughter cells that differ in morphology and polar features: a sessile stalked cell and a motile swarmer cell that subsequently differentiates into a stalked cell. In this work we investigate the assembly of the Caulobacter cell division machinery (the divisome) using genetics, biochemistry, and microscopy. In Caulobacter, the cell division process requires a set of approximately twenty-three proteins localizing from the cytoplasm to the outer membrane. To understand divisome assembly as a function of the cell cycle, we generated fluorescent fusions to analyze the temporal regulation of 19 representative divisome and division-site localized proteins. In Chapter 2, we identified a series of stages and transitions in divisome assembly and the associated events yielding a comprehensive temporal picture of the process. The assembly interdependency for divisome formation in Caulobacter appears to involve cooperative rather than sequential recruitment, suggesting that it is a multiprotein subcomplex model. In Chapter 3, we describe our investigation of the Tol-Pal complex where we demonstrated that it plays a vital role for membrane integrity maintenance and that it is essential for viability. Cryo-electron microscope images of the Caulobacter cell envelope exhibited outer membrane disruption, and cells failed to complete cell division in TolA, TolB, or Pal mutant strains. The Tol-Pal complex is required to maintain the position of the transmembrane TipN polar marker, and indirectly the PleC histidine kinase, at the cell pole, but it is not required for the polar maintenance of other transmembrane and membrane-associated polar proteins tested. Thus, the Caulobacter trans-envelope Tol-Pal complex is a key component of cell envelope structure and function, mediating outer membrane constriction at the final step of cell division, as well as the positioning of a protein localization factor. In Chapter 4, we describe our examination of the FtsZ binding protein, ZapA. FtsZ is the most highly conserved divisome protein that polymerizes into a contractile ring near midcell, defining the future site of cell division. We showed that ZapA is required to maintain a normal cell length, and promotes Z ring assembly. The biochemical and functional studies suggest that Caulobacter ZapA is a positive regulator of Z-ring assembly. In summary, we have addressed three major stages in developments of the divisome in Caulobacter: Z-ring assembly, divisome maturation and outer membrane invagination. These experiments have provided a new understanding of how the Caulobacter cell temporally executes the cell division program to propagate reliably and how Caulobacter cell division is performed
The story of single molecules, from early spectroscopy in solids to super-resolution nanoscopy in cells and beyond by W. E Moerner( Visual )

2 editions published in 2015 in English and held by 2 WorldCat member libraries worldwide

(CIT): Special NIH Nobel Laureate Lecture Dr. W.E. Moerner, who shared the 2014 Nobel Prize in chemistry for the development of super-resolution fluorescence microscopy, will discuss his pioneering work in a special NIH lecture titled "The Story of Single Molecules, from Early Spectroscopy in Solids to Super-Resolution Nanoscopy in Cells and Beyond."
Single molecules and atoms( Book )

1 edition published in 1996 in English and held by 2 WorldCat member libraries worldwide

Development of techniques for live cell RNA imaging by Jungjoon Kempthorne Lee( )

1 edition published in 2011 in English and held by 2 WorldCat member libraries worldwide

The development of live cell RNA imaging techniques will lead to the unraveling of many important biological processes. To achieve this goal, there have been three different strategies developed. They are the development of small molecule probes, nucleic acid probes, and green fluorescent protein (GFP) probes. In the following thesis, the pros and cons of each approach are discussed, followed by a proposal to resolve the limitations. In the small molecule case, a probe was developed that utilized a quenched sulforhodamine dye. It was designed so that its structure can be rationally modified from the initial lead compound. An aptamer sequence that activates the sulforhodamine probe with micro molar affinity was found by in vitro Systematic Evolution of Ligands by Exponential Enrichment (SELEX), followed by fluorescence screening in E.coli. The rational modification of the structure of the initial sulforhodamine probe resulted in an overall 33-fold increase in binding affinity compared to the initial lead compound. Instead of the chemical modification of the lead compound, the small molecule's cell permeability and binding affinity to the target could be improved by linking to cell penetrating peptides (CPP). A CPP is a short peptide sequence composed of poly arginine amino acids which shows excellent cell uptake and affinity to RNA. However, the use of the CPP-linked dye in live cell imaging has been limited by strong signals in the endosome region. An attempt was made to overcome this difficulty by linking a quencher molecule to the dye-CPP via a disulfide bond, which only breaks when it enters the cytosol. For the nucleic acid probe, the major problem was its low cell permeability and low signal-to-background ratio due to the low copy number of mRNA targets within the cell. We made mutant Hammerhead ribozymes and embedded them in a non-coding region of the GFP expression vector that can be transfected to mammalian cells. This modified Hammerhead ribozyme acts as a logic gate, and the signal is amplified by the expression of GFP in the presence of the target mRNA. In vitro and in vivo results are discussed. Finally, a fragmented GFP system, the fluorescence of which could be recovered by binding to a specific RNA tag, was developed. The major problem for the GFP-mediated RNA imaging system was the low signal-to-background ratio from the GFP probe that is not bound to the RNA tag. To find the non-fluorescent GFP, the GFP was truncated from the C-terminus such that it loses its fluorescence with minimum loss of amino acids. An RNA sequence that has high affinity to this GFP was found by in vitro SELEX. The subsequent E.coli screening found an RNA sequence that reactivates the fluorescence of the GFP probe
Fluorophores for single-molecule imaging in living cells : characterizing and optimizing DCDHF photophysics by Samuel Joseph Lord( )

1 edition published in 2010 in English and held by 2 WorldCat member libraries worldwide

The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. There is a need for new fluorophores that can be used for single-molecule imaging in biological media, because imaging in cells and in organisms require emitters that are bright and photostable, red-shifted to avoid pumping cellular autofluorescence, and chemically and photophysically tunable. To this end, we have designed and characterized fluorescent probes based on a class of nonlinear-optical chromophores termed DCDHFs. This Dissertation describes various physical and optical studies on these emitters, from sensing local environment to photoactivation. Chapter 1 is a general introduction to fluorescence and single-molecule spectroscopy and imaging. Single-molecule experiments in living cells are discussed and probes used for such experiments are summarized and compared. Chapter 2 explores the basic photophysics of the DCDHF fluorophores and some general methods of measuring relevant spectroscopic parameters, including photostability. Chapter 3 discusses the various approaches we have taken to modify particular properties by changing the fluorophore's structure. We have redesigned the DCDHF fluorophore into a photoactivatable fluorogen--a chromophore that is nonfluorescent until converted to a fluorescent form using light--described in Chapter 4. Finally, a different, chemical route to fluorescence activation is presented in Chapter 5. The remainder of the Dissertation is the Appendix and a full Bibliography. The Appendix includes a table of photophysical parameter for DCDHF fluorophore, various protocols used in the experiments discussed, MatLab codes, and NMR spectra
Dynamical Hole-Burning Requirements for Frequency Domain Optical Storage( Book )

1 edition published in 1986 in English and held by 1 WorldCat member library worldwide

Persistent spectral hole burning (PHB) is a frequency selective bleaching phenomenon that potentially provides a new dimension for optical storage of information, if suitable materials can be found. In the most general form of PHB, narrow depressions or holes are formed (burned) in an inhomogeneously broadened absorption line of an absorber in a solid at low temperatures whenever light absorption causes a change in the absorbing center that persists longer than any excited state lifetime. Previously studied materials involve monophotonic mechanisms for the photochemical or photophysical change leading to hole formation; such mechanisms have serious limitations as to the number of reads that can be performed without distortion of the written data. Recently, photon gated spectral hole burning has been observed in both inorganic and organic materials, indicating that nondestructive reading of spectral holes is possible. This talk will focus on the properties of the organic photon gated material in particular and describe the properties of what might be termed an ideal photon gated system. Keywords: Frequency domain optical storage, Photon gating, Biphotonic, and Nondestructive reading
Single photons on demand from a single molecule at room temperature by B Lounis( )

1 edition published in 2000 in English and held by 1 WorldCat member library worldwide

Reading and Writing of Photochemical Holes Using GaA1As Diode Lasers( Book )

1 edition published in 1983 in English and held by 1 WorldCat member library worldwide

A current tuned (gallium aluminum arsenide) diode laser is utilized both to burn and to detect narrow photochemical holes in the inhomogeneously broadened 833 nm zero phonon line of the R' color center in LiF. Applications for reading and writing data into frequency domain optical memories based on photochemical hole burning are discussed. (Author)
Mechanisms of Photorefractivity in Polymeric Materials( )

4 editions published between 1999 and 2004 in English and held by 0 WorldCat member libraries worldwide

This six month grant provided a continuation at Stanford University of AFOSR Grant No. F49620-96-1-0135, which ended 31 Mar 1999 at the University of California, San Diego (UCSD). During the period of this grant, accomplishments are of note. A major study of the effect of nonlinear chromophore energetic on the several speed of photorefractive polymers showed that rational design can improve the properties of these materials. Second, the entire laboratory moved from UCSD to Stanford University in July 1999. During this time, a major review article on photorefractive polymers for the Encyclopedia of Materials Science and Technology was completed (Pub. 171). Finally, in the new laboratories at Stanford, a first demonstration of fast image amplification with photorefractive polymers was completed
Ultrasensitive Laser Spectroscopy in Solids: Single-Molecule Detection( )

2 editions published between 1989 and 1990 in English and held by 0 WorldCat member libraries worldwide

In spite of detection intensity constraints necessary to avoid power broadening, the optical absorption spectrum of single molecules of pentacene in p-terphenyl crystals can be measured by (1) using laser FM spectroscopy combined with Stark and/or ultrasonic double modulation (to remove residual amplitude modulation) and (2) recording spectra far out in the wings of the inhomogeneous line to reduce the number of molecules in resonance to one
Single-molecule optical detection, imaging and spectroscopy by W. E Moerner( )

3 editions published between 1996 and 2008 in English and held by 0 WorldCat member libraries worldwide

Single Molecule Spectroscopy is one of the hottest topics in today's chemistry. It brings us close to the the most exciting vision generations of chemists have been dreaming of: To observe and examine single molecules!. While most of chemistry deals with myriads of molecules, this books presents the latest developments for the detection and investigation of single entities. Written by internationally renowned authors, it is a thorough and comprehensive survey of current methods and their applications
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Alternative Names
Gulielmus Moerner

Moerner, W. E.

Moerner, William 1953-

Moerner, William E. 1953-

Moerner, William E. (William Esco), 1953-

Moerner, William Esco 1953-

W. E. Moerner

W. E. Moerner biochimico statunitense

W·E·莫尔纳尔 美国化学家

William E. Moerner amerikansk fysikar og kjemikar

William E. Moerner amerikansk fysiker och kemist

William E. Moerner amerikansk fysiker og kemiker

William Esco Moerner

William Esco Moerner amerikansk fysiker og kjemiker

William Moerner Amerikaans natuurkundige

William Moerner US-amerikanischer Physiker und Chemiker

Вилијам А. Морнер американски хемиски физичар и нобеловец

Вільям Мернер

Мёрнер, Уильям

وليام مورنر

ولیم ای مورنر

ولیم مورنر

ویلیام اسکو مورنر شیمی‌دان و فیزیک‌دان آمریکایی

विलियम एस्को मोइरनर

विल्यम मोएर्नर

विलियम मर्नर

উইলিয়াম এসকো মোয়ের্নার

வில்லியம். ஈ. மோர்னர்

വില്ല്യം.ഇ.മോണർ അമേരിക്കൻ കെമിസ്റ്റ്

უილიამ მიორნერი

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English (43)

German (2)