WorldCat Identities

Arnold, Frances Hamilton

Overview
Works: 51 works in 121 publications in 1 language and 1,256 library holdings
Genres: Laboratory manuals  Academic theses  Methods (Music)  Songs and music 
Roles: Author, Thesis advisor, Editor, Composer, Other, Librettist
Publication Timeline
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Most widely held works by Frances Hamilton Arnold
Directed evolution library creation : methods and protocols by Frances H Arnold( )

13 editions published in 2003 in English and held by 362 WorldCat member libraries worldwide

Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Protocols, seasoned practitioners from many leading laboratories share their best experimental protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications
Directed enzyme evolution : screening and selection methods by F. H Arnold( )

15 editions published in 2003 in English and held by 357 WorldCat member libraries worldwide

Directed evolution, the application of evolutionary design to enzyme engineering, requires effective screening strategies to isolate those proteins that perform a desired function from the libraries generated by the techniques. In Directed Enzyme Evolution: Screening and Selection Methods, seasoned practitioners from many leading laboratories describe their leading and readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. There are also representative examples of how phage libraries may be interrogated for enzymatic activity. Each protocol contains detailed step-by-step instructions and many notes on how best to deal with the problems that may occur. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Taken together, Directed Enzyme Evolution: Screening and Selection Methods and Directed Evolution Library Creation: Methods and Protocols capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications
Evolutionary protein design( )

6 editions published between 2000 and 2001 in English and held by 308 WorldCat member libraries worldwide

This is the first high-quality, comprehensive overview of the field of evolutionary protein design. Topics include new protein design strategies, the structures of laboratory-evolved proteins, the evolution of non-natural enzyme functions, and the theory of laboratory evolution
New enzymes for organic synthesis : screening, supply and engineering by Thomas Scheper( Book )

10 editions published in 1997 in English and held by 148 WorldCat member libraries worldwide

Isolated enzymes are known as useful for synthesising of complex molecules with more and more large scale applications in recent years. These six reviews show different steps in the development of enzymatic syntheses for useful compounds. Screening of novel enzymes, their biochemical characterisation as well as reaction engineering approaches in order to increase their performance are treated in three papers. Then the use of protein design as a tool for the directed improvement of reactivity and selectivity of biocatalysts is described in two reviews. The supply of sufficient quantities of biocatalysts must not be neglected: fluidized bed adsorption as an emerging technology for the simple and fast purification of biomolecules is presented in the light of its potential in large scale supply of enzyme catalysts
Flora of Sussex; or, A list of the flowering plants & ferns found in the county of Sussex, with localities of the less common species by Frederick Henry Arnold( Book )

7 editions published between 1907 and 2015 in English and held by 10 WorldCat member libraries worldwide

Metal affinity protein separations( Book )

2 editions published in 1992 in English and held by 8 WorldCat member libraries worldwide

Design and scale-up of affinity separations by Frances Hamilton Arnold( )

3 editions published in 1985 in English and held by 7 WorldCat member libraries worldwide

Growth of the genetic-engineering-based biochemical industry has stimulated the demand for new protein and peptide separation techniques mild enough to maintain delicate native conformations, yet powerful enough to achieve the high purities required for clinical use. Bioselective adsorption, also known as affinity chromatography, is used extensively in research laboratories; recently it has been used in commerical purifications as well. Both adsorbent and feed in affinity separations are often very costly, and it is important to be able to predict the performance of commerical-scale systems reliably, based on data from laboratory-scale experiments. This is the central problem of the thesis. To this end, experiments to identify and quantify the rate-limiting step(s) for affinity adsorption have been carried out on two model systems: immobilized monoclonal antibodies binding to soluble antigen and (2) immobilized blue dye binding to albumin. The governing equations have been solved for a number of cases for affinity adsorption, wash, and elution. A pulse technique for measuring mass transfer rates independently of the biological interaction is described. From this work on affinity purifications it has become clear that apart from its utility as a separation technique affinity chromatography is a powerful analytical tool for the study of biological interactions. Affinity experiments can be used to measure the equilibrium constants and kinetics of biological binding processes. Zonal and frontal analytical affinity chomatography theory is discussed
Penmanship stories : a teacher's handbook by Frank H Arnold( Book )

3 editions published between 1923 and 1930 in English and held by 6 WorldCat member libraries worldwide

Ahab : an oratorio by George Benjamin Arnold( )

1 edition published in 1864 in English and held by 5 WorldCat member libraries worldwide

The kinetics of the radition induced decomposition of sodium nitrate by Arnold Frances( )

4 editions published in 1974 in English and held by 3 WorldCat member libraries worldwide

The influence of spacing upon branch and knot development in pure plantations of white, red and scotch pine by F. H Arnold( Book )

2 editions published in 1932 in English and held by 2 WorldCat member libraries worldwide

Innovation by evolution. by Frances Hamilton Arnold( Visual )

1 edition published in 2008 in English and held by 2 WorldCat member libraries worldwide

Directed evolution of biosynthetic pathways to carotenoids with unnatural carbon backbones by Alexander Vincent Tobias( Book )

1 edition published in 2006 in English and held by 2 WorldCat member libraries worldwide

Design and analysis of combinatorial protein libraries created by site-directed recombination by Jeffrey B Endelman( Book )

1 edition published in 2005 in English and held by 2 WorldCat member libraries worldwide

Directed Evolution of Novel Enzyme Activities( Book )

2 editions published in 2002 in English and held by 2 WorldCat member libraries worldwide

We report here the directed evolution of the two valuable oxidases horseradish peroxidase (HRP) and laccase. We achieved functional expression of HRP in S. cerevisiae, and in E. coli to an extent that allows the improvement of this important catalyst. Mutagenesis, recombination and screening were successful in increasing total activity 40 fold by improving expression, catalytic activity and thermostability. Mutants were produced at up to 600 U/I/OD in Pichia pastoris. We also used computation-guided saturation mutagenesis to create higher active and stabilized HRP mutants more efficiently. We expressed the laccase from Myceliophthora themophila in Saccharomyces cerevisiae. We employed directed evolution to improve catalysis as well as expression level to a 170 fold higher total activity. Our evolved yeast mutant has the highest functional expression ever reported for laccase in a nonfungal system. Kinetic characterization of the purified mutants showed that the substrate specificity was left unchanged. The compromised thermostability of the mutants was regained in only one generation of stability screening. For the functional improvement of laccase we developed high throughput assays. PAH bioremediation can be directly assessed with anthracene and for highest sensitivity (0.3 mU/ml) Poly-R, a readily soluble surrogate substrate for PAHs can be used
Petworth, a sketch of its history and antiquities : with notices of objects of archaeological interest and its vicinity by F. H Arnold( Book )

1 edition published in 1864 in English and held by 2 WorldCat member libraries worldwide

Enzyme design by directed evolution by Huimin Zhao( Book )

1 edition published in 1998 in English and held by 2 WorldCat member libraries worldwide

Expression and Directed Evolution of Peroxidase Enzymes( Book )

2 editions published in 2000 in English and held by 2 WorldCat member libraries worldwide

The overall goal of this research is to develop strategies for the evolution of novel functions in proteins. The main objective of this project has been to realize the functional expression of eukaryotic peroxidases in facile recombinant hosts (bacteria and yeast), which is a prerequisite to tailoring these enzymes for industrial and Naval applications. We have improved the folding and expression of horseradish peroxidase in recombinant hosts by directed evolution
Engineering dioxygenases by laboratory evolution : a comparison of evolutionary search strategies by John M Joern( Book )

1 edition published in 2003 in English and held by 2 WorldCat member libraries worldwide

Metal Mediated Two-Dimensional Protein Crystallization( Book )

2 editions published in 1999 in English and held by 2 WorldCat member libraries worldwide

We have developed a microfabricated system for sorting cells for
 
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Directed evolution library creation : methods and protocols
Covers
Directed enzyme evolution : screening and selection methodsNew enzymes for organic synthesis : screening, supply and engineering
Alternative Names
Arnold, F. H.

Arnold, F. H. 1956-

Arnold, Frances H.

Arnold, Frances H. 1956-

Frances Arnold Amerikaans biochemica

Frances Arnold Amerikaanse biochemicus en Nobelprijswinnaar

Frances Arnold biochimica e ingegnera statunitense

Frances Arnold chimiste américaine

Frances Arnold química estadounidense

Frances Arnold yhdysvaltalainen tieteilijä, biokemian insinööri

Frances Arnoldová americká biochemička

Frances H. Arnold amerikansk biokemiker

Frances H. Arnold amerikansk biokemist

Frances H. Arnold US-amerikanische Biochemikerin

Frances H. Arnold US-amerikanische Biochemikerin und Nobelpreisträgerin

Hamilton Arnold, Frances

Hamilton Arnold, Frances 1956-

Арнольд, Фрэнсис

Франсис Арнолд

Френсис Арнолд американска биохемичарка и нобеловка

Френсис Арнолд амерички хемијски инжењер

Френсіс Арнольд американський біохімік та інженер, нобелівський лауреат (2018)

Фрәнсис Арнольд

Ֆրենսիս Առնոլդ

פרנסס המילטון ארנולד מדענית אמריקאית, כלת פרס נובל לכימיה 2018

فرانسيس أرنولد عالمة كيمياء أمريكية

فرانسیس آرنولد

फ्रांसेस आर्नोल्ड

ফ্রান্সিস আর্নল্ড

பிரான்செசு ஆர்னோல்டு

아널드, 프랜시스 H. 1956-

아널드, 프랜시스 해밀턴 1956-

프랜시스 아널드 미국의 화학공학자

フランシズ・アーノルド アメリカ合衆国の科学者、工学者

弗朗西丝·阿诺德

弗朗西斯·阿諾德

Languages
English (78)