WorldCat Identities

DeLucas, Lawrence J.

Overview
Works: 7 works in 22 publications in 2 languages and 281 library holdings
Roles: Editor, Author, Thesis advisor
Publication Timeline
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Most widely held works by Lawrence J DeLucas
Membrane protein crystallization by Lawrence J DeLucas( )

11 editions published between 2009 and 2014 in English and held by 269 WorldCat member libraries worldwide

Discusses challenges of membrane protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This title includes tools to enhance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes
Crystallization, X-Ray Structure Determination and Structure-Based Drug Design for Targeted Malarial Enzymes( Book )

6 editions published between 1997 and 1999 in English and held by 6 WorldCat member libraries worldwide

The aim of this project is to identify specific inhibitors of some enzymes of malaria parasite. Inhibitors will be designed based on the high-resolution crystal structure of individual target enzymes. We have already determined the crystal structure of P. falciparum lactate dehydrogenase, a key enzyme in the glycolytic pathway, to high resolution. This structure is currently being used for designing lead inhibitors. We have also purified PFPK-DHPS bifunctional enzyme for structure analysis and are presently screening for crystallization conditions. We have also optimized the purification of P. falciparum cyclophilin. We have also crystallized P. falciparum Rab6 for structure determination. This is the first Rab protein of the malaria parasite to be crystallized
Initial crystallographic analysis of human serum transferrin by Lawrence J DeLucas( )

1 edition published in 1982 in English and held by 2 WorldCat member libraries worldwide

Kristallisation regulatorischer RNA-Moleküle unter Mikrogravitationsbedingungen : Abschlussbericht by V. A Erdmann( Book )

1 edition published in 2010 in German and held by 1 WorldCat member library worldwide

The crystal structure of the Yersinia pestis iron chaperone YiuA reveals a basic triad binding motif for the chelated metal( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

Abstract : YiuA, a Yersinia pestis substrate-binding protein, contains a putative basic triad binding motif in the canonical substrate-binding site, indicative of a metal-chelate complex binding site. Additional structural and simulation analyses support the putative function of YiuA binding bacterial siderophores. Abstract : Biological chelating molecules called siderophores are used to sequester iron and maintain its ferric state. Bacterial substrate-binding proteins (SBPs) bind iron-siderophore complexes and deliver these complexes to ATP-binding cassette (ABC) transporters for import into the cytoplasm, where the iron can be transferred from the siderophore to catalytic enzymes. In Yersinia pestis, the causative agent of plague, the Yersinia iron-uptake (Yiu) ABC transporter has been shown to improve iron acquisition under iron-chelated conditions. The Yiu transporter has been proposed to be an iron-siderophore transporter; however, the precise siderophore substrate is unknown. Therefore, the precise role of the Yiu transporter in Y. pestis survival remains uncharacterized. To better understand the function of the Yiu transporter, the crystal structure of YiuA (YPO1310/y2875), an SBP which functions to present the iron-siderophore substrate to the transporter for import into the cytoplasm, was determined. The 2.20 and 1.77 Å resolution X-ray crystal structures reveal a basic triad binding motif at the YiuA canonical substrate-binding site, indicative of a metal-chelate binding site. Structural alignment and computational docking studies support the function of YiuA in binding chelated metal. Additionally, YiuA contains two mobile helices, helix 5 and helix 10, that undergo 2-3 Å shifts across crystal forms and demonstrate structural breathing of the c-clamp architecture. The flexibility in both c-clamp lobes suggest that YiuA substrate transfer resembles the Venus flytrap mechanism that has been proposed for other SBPs
Current topics in membranes( Book )

1 edition published in 2009 in English and held by 1 WorldCat member library worldwide

Discusses challenges of membrane protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This title includes tools to enhance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes
Development of a high-throughput self-interaction chromatography system by David H Johnson( )

1 edition published in 2014 in English and held by 1 WorldCat member library worldwide

The first recombinant human protein drug, insulin expressed in e.coli cells, was approved by the FDA in 1982. Since then, protein therapeutics have become the fastest growing segment of the pharmaceutical industry and include immunoglobulin-g (IgG) directed cancer and immune disorder treatments. A major difficulty to bring protein drugs to market is the requirement that they be concentrated up to 150 mg/ml without aggregation for efficacy in a small injection volume. One way to improve protein drug solubility is to include additives that reduce protein-protein attraction and increase protein-protein repulsion thereby preventing protein molecules from coming together to form aggregates. However, hundreds of individual additives are approved by the FDA for injection and just ten additives at four possible concentration levels provides over a million (410) possible formulations. To address this formulation search problem, automated hardware and screening techniques are applied to evaluate the effect of additives on protein-protein interactions. These interactions are quantified by the second virial coefficient (B value), a thermodynamic parameter that is the sum of forces between two protein molecules at all orientations and distances in a solution. B values are measurements made by self-interaction chromatography. Contributions to the formulation search problem include hardware, software and screen methodology improvements. The hardware consists of 1) robotic formulation delivery and system equilibration, 2) a reduced-cost flow cell design with protein detection utilizing UV LEDs and 3) a multi-column system for parallel experimentation. The software includes two parts 1) automation of the self-interaction chromatography experiment and 2) a neural network model of additive influence on protein-protein interactions. The hardware and software components are utilized in a tiered additive screen including individual additive evaluation (initial screen), a complex formulation evaluation (incompletefactorial) and training of a neural network to model the additive influence on proteinprotein interactions. The neural-network model is then used to predict the B value of additive combinations not previously measured and the prediction capability of the model is evaluated. The system was evaluated using an IgG drug candidate protein from Minerva Biotechnologies and predictions from the neural network produced a 100 fold increase in solubility
 
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Membrane protein crystallization Current topics in membranes
Covers
Current topics in membranes
Alternative Names
DeLucas, Larry

Lawrence DeLucas biochimico e astronauta statunitense

Lawrence J. DeLucas Amerikaans ruimtevaarder

Lawrence J. DeLucas amerikansk astronaut

Lawrence J. DeLucas chimiste américain

Lawrence J. DeLucas US-amerikanischer Astronaut

Lawrence James DeLucas amerikai űrhajós

Делукас, Лоуренс Джеймс

Лорънс Де Лукас

Лоуренс Джеймс Делукас

لورنس جی دلوکاس فضانورد آمریکایی

Languages
English (21)

German (1)