WorldCat Identities

Dorman, Robert V.

Overview
Works: 2 works in 3 publications in 1 language and 3 library holdings
Publication Timeline
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Most widely held works by Robert V Dorman
Description and Manipulation of Membrane Lipid Alterations Associated with Synaptic Functions in Isolated Cerebellar Glomeruli( Book )

1 edition published in 1989 in English and held by 1 WorldCat member library worldwide

Isolated cerebellar and Hippocampal mossy fiber synaptosomes were used to assess the relationships between membrane lipid metabolism and the evoked release of excitatory amino acid neurotransmitters. Mossy fiber terminals were radio-labeled with arachidonic acid and the effects of membrane depolarization and calcium influx on the labeling of the component lipid pools were determined. It was observed that depolarization and Ca2+ influx stimulated the accumulation of unesterified arachidonate. This effect was correlated with increased production of prostaglandins, and lipoxygenase inhibitors. The depolarization and arachidonate-induced transmitter release was mimicked by the addition of PGE2a and blocked by the cyclooxygenase inhibitor ibuprofen. The correlation of arachidonic acid metabolism with prostaglandin synthesis, ATPase inhibition and Ca2+ mobilization, suggests they may all play a role in the mechanisms of neurotransmitter release. (AW)
Involvement of Lipid Metabolism in Chemical Transmission Processes at Mossy Fiber Synapses( )

2 editions published between 1992 and 1993 in English and held by 0 WorldCat member libraries worldwide

Isolated hippocampal mossy fiber synaptosomes were used to assess the relationships between membrane lipid metabolism and the evoked release of the excitatory amino acid neurotransmitter glutamate. A variety of metabolic parameters were investigated in order to develop a comprehensive model for the mechanisms of transmitter release. Mossy fiber terminals were radiolabeled with 3H arachidonic acid and the effects of membrane depolarization and calcium influx on the labeling of the component lipid pools were determined. It was observed that depolarization and Ca2+ influx stimulated the accumulation of unesterified arachidonate. This effect was correlated with increased production of prostaglandins, in particular PGF2a and could be blocked with lipase inhibitors and Ca2+ channel blockers. Prostaglandin production was also blocked by cyclooxygenase and lipoxygenase inhibitors. We also observed that exogenous arachidonate stimulates the release of endogenous glutamate from mossy fiber terminals, as well as the mobilization of intraterminal free Ca2+. In addition we identified two naturally occurring products which act synergistically to activate protein kinase C and facilitate the evoked release of glutamate
 
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