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| Genre: | Internetbron |
|---|---|
| Soort document: | Boek, Internetbron |
| Alle auteurs / medewerkers: |
Richard J Simpson; Peter D Adams; Erica Golemis |
| ISBN: | 9780879698683 0879698683 9780879698676 0879698675 |
| OCLC-nummer: | 235945971 |
| Beschrijving: | vii, 436 p. : ill. ; 29 cm. |
| Inhoud: | Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks. Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest. |
| Verantwoordelijkheid: | edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis. |
| Meer informatie: |
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Vergelijkbare items
Verwante onderwerpen:(7)
- Proteins -- Purification -- Laboratory manuals.
- Proteins -- Analysis -- Laboratory manuals.
- Proteins -- isolation & purification -- Laboratory Manuals.
- Chromatography -- methods -- Laboratory Manuals.
- Electrophoresis -- methods -- Laboratory Manuals.
- Immunoprecipitation -- methods -- Laboratory Manuals.
- Proteins -- analysis -- Laboratory Manuals.