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| Dokumenttyp: | Buch |
|---|---|
| Alle Autoren: |
Jeffrey W Pollard; John M Walker |
| ISBN: | 0896034410 9780896034419 0896033848 9780896033849 |
| OCLC Nummer: | 37902587 |
| Anmerkungen: | Previous edition published as: Animal Cell culture, 1990. |
| Beschreibung: | xiv, 489 p. : ill. ; 24 cm. |
| Inhalt: | Basic cell culture -- Establishment, maintenance, and cloning of human dermal fibroblasts -- Aging of cultured human skin fibroblasts -- Ex vivo maintenance of differentiated mammalian cells -- Scale-up of suspension and anchorage-dependent animal cells -- Hollow-fiber cell culture -- Separation and maintenance of primary T- and B-lymphocytes -- Human pilosebaceous culture -- Keratinocyte culture -- Tissue culture of skeletal muscle -- Isolation of rat liver hepatocytes -- Primary kidney cells -- Human thyroid epithelial cells -- Derivation and maintenance of embryonic stem cell cultures -- Culture of cells from human tumors of the nervous system on an extracellular matrix derived from bovine corneal endothelial cells -- Culturing primitive hemopoietic cells -- Collagen gel culture of the human hematopoietic progenitors cFU-GM, CFU-E, and BFU-E -- Long-term B-lymphoid cultures from murine bone marrow establishment and coloning by using stomal cell line AC 6.21 -- Human long-term bone marrow culture -- In vitro clonal culture of human hematopoietic progenitor cells -- High proliferative potential colony-forming cells -- Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors -- Flow sorting for isolating CFU-E -- Murine bone marrow-derived macrophages -- Routine testing of cell cultures and their products for mycoplasma contamination -- Fluorescent in situ hybridization (FISH) for DNA probes in the interphase and metaphases stages of the cell cycle -- Cytogenetic techniques for human leukemias -- Visualizaiton of cell replication using antibody to proliferating cell nuclear antigen -- Proliferation of murine myoblasts as measured by BrdUrd incorporation -- Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method -- nRNA in situ hybridization to in vitro cultured cells -- Transmission and scanning electron microscope preparation of whole cultued cells -- Adhesion molecules on isolated and cultured microvascular endothelial cells demonstrated by immunofluorescence and immune electron microscopy -- Producation of heterologous proteins using the baculovirus/insect expression system -- Transfection and transformation of human thyroid epithelial cells -- Analyzing chemotaxis using the Dunn direct-viewing chamber -- Computer-assisted analysis of single-cell behavior. |
| Serientitel: | Methods in molecular biology (Clifton, N.J.), v. 75. |
| Verfasserangabe: | edited by Jeffrey W. Pollard and John M. Walker. |
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