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3D cell culture : methods and protocols

Author: John W Haycock
Publisher: New York : Humana Press, ©2011.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 695.
Edition/Format:   Print book : EnglishView all editions and formats
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Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained  Read more...

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Genre/Form: Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: John W Haycock
ISBN: 9781607619833 1607619830 9781607619840 1607619849
OCLC Number: 681733176
Description: xi, 343 pages : illustrations ; 27 cm.
Contents: 3D cell culture : a review of current approaches and techniques / John W. Haycock --
Scaffolds for tissue engineering and 3D cell culture / Eleonora Carletti, Antonella Motta, and Claudio Migliaresi --
Tracking nanoparticles in three-dimensional tissue-engineered models using confocal laser scanning microscopy / Vanessa Hearnden, Sheila MacNeil, and Giuseppe Battaglia --
Using immuno-scanning electron microscopy for the observation of focal adhesion-substratum interactions at the nano- and microscale in S-phase cells / Manus J. Biggs, R. Geoff Richards, and Matthew J. Dalby --
3D sample preparation for orthopaedic tissue engineering bioreactors / Sarah H. Cartmell [and others] --
Quantification of mRNA using real-time PCR and western blot analysis of MAPK events in chondrocyte/agarose constructs / David A. Lee [and others] --
Genetic modification of chondrocytes using viral vectors / Teresa Coughlan [and others] --
Stem cell and neuron co-cultures for the study of nerve regeneration / Paul J. Kingham, Cristina Mantovani, and Giorgio Terenghi --
Production of tissue-engineered skin and oral mucosa for clinical and experimental use / Sheila MacNeil, Joanna Shepherd, and Louise Smith --
Three-dimensional alignment of Schwann cells using hydrolysable microfiber scaffolds : strategies for peripheral nerve repair / Celia Murray-Dunning [and others] --
Encapsulation of human articular chondrocytes into 3D hydrogel : phenotype and genotype characterization / Rui C. Pereira [and others] --
Micro-structured materials and mechanical cues in 3D collagen gels / James B. Phillips and Robert Brown --
Organotypic and 3D reconstructed cultures of the human bladder and urinary tract / Claire L. Varley and Jennifer Southgate --
Ex vivo organ culture of human hair follicles : a model epithelial-neuroectodermal-mesenchymal interaction system / Desmond J. Tobin --
Human endothelial and osteoblast co-cultures on 3D biomaterials / Ronald E. Unger [and others] --
Assessment of nanomaterials cytotoxicity and internalization / Noha M. Zaki and Nicola Tirelli --
Practical aspects of OCT imaging in tissue engineering / Stephen J. Matcher --
Osteogenic differentiation of embryonic stem cells in 2D and 3D culture / Lee Buttery [and others] --
3D structuring of biocompatible and biodegradable polymers via stereolithography / Andrew A. Gill and Frederik Claeyssens --
Alvatex® : polystyrene scaffold technology for routine three dimensional cell culture / Eleanor Knight [and others].
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 695.
Other Titles: Three-dimensional cell culture
Responsibility: edited by John W. Haycock.

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From the reviews:"This book is an excellent resource to help researchers find practical information to enable them to develop three-dimensional cell culture systems. ... anyone who wishes to enter Read more...

 
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