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Adenovirus methods and protocols

Author: William S M Wold
Publisher: Totowa, NJ : Humana Press, ©1999.
Series: Methods in molecular medicine, 21.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

A collection of readily-reproducible methods for conducting research with adenoviruses.

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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Adenovirus methods and protocols.
Totowa, NJ : Humana Press, ©1999
(DLC) 98029395
(OCoLC)39477736
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: William S M Wold
ISBN: 9781592596034 1592596037
OCLC Number: 54639526
Language Note: English.
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xiii, 352 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Preparation and titration of CsCI-banded adenovirus stock / Ann E. Tollefson, Terry W. Hermiston, and William S.M. Wold --
Construction of mutations in adenovirus early region 3 (E3) transcription units / Terry W. Hermiston, Ann E. Tollefson, and William S.M. Wold --
Isolation, growth, and purification of defective adenovirus deletion mutants / Gary Ketner and Julie Boyer --
Manipulation of early region 4 / Julie Boyer and Gary Ketner --
Adenovirus DNA packaging: construction and analysis of viral mutants / Susanne I. Schmid and Patrick Hearing --
Methods for creating and anlyzing adenovirus vectors that express proteins that act on the viral genome / C.S.H. Young, Andrea L. Nicolás, Heng Lu, and Patricia L. Munz --
Construction of mouse adenovirus type 1 mutants / Angela N. Cauthen and Katherine R. Spindler --
Generation of adenovirus-specific cytotoxic T lymphocytes / Time E. Sparer and Linda R. Gooding --
Measurement of macrophage-mediated cytolysis of adenovirus-infected cells --
Penelope J. Duerksen-Hughes and Linda R. Gooding --
Measurement of tumor necrosis factor (TNF) lysis of adenovirus-infected cells / Joanna L. Shisler and Linda R. Gooding --
Role of natural killer (NK) cells in immunity to adenoviruses / John M. Routes --
Determination of the transforming activities of adenovirus oncogenes / T. Subramanian and G. Chinnadural --
Cell survival and apoptosis assays for E1B 19K and Bcl-2 family members / T. Subramanian and G. Chinnadural --
In vitro transcription: probing the molecular functions of adenovirus regulatory proteins / Paul M. Loewenstein, Chao-Zhong Song, and Maurice Green --
Cell microinjection: in vivo analysis of the functional domains of viral regulatory proteins / Maurice Green, Andrew Thorburn, and Paul M. Loewenstein --
Immunoprecipitation of E1A-containg protein complexes / Elizabeth Moran and Peter Yaciuk --
Preparaton of splicing-competent nuclear extracts from adenovirus-infected cells / Oliver M̈ühlemann and Göran Akusjärvi --
Adenovirus entry into cells: a quantitative fluorescence microscopy approach / Urs F. Greger, Michel Y. Nakano, and Maarit Suomalainen --
Simultaneous detection of RNA and proteins in adenovirus-infected cells by fluorescence in situ hybridization (FISH) and immunostaining / Eileen Bridge --
Characterization of the adenovirus fiber protein / Jeffrey A. Engler, Barbara Mulach, and Jeong Shin Hong --
Large-scale purification and crystallization of adenovirus hexon / John J. Rux, Donatella Pascolini, and Roger M. Burnett --
Adenovirus protease / Joseph M. Weber --
Methods for growth and purification of enteric adenovirus type 40 / Vivien Mautner --
Transfection complexes generated with adenovirus and polyethylenimine-condensed DNA / Matt Cotten, Mediyha Saltik, and Adam Baker --
Phylogenetic analysis of adenovirus sequences: proof of the necessity of establishing a third genus in the adenoviridae family / Balázs Harrach and Mária Benkö
Series Title: Methods in molecular medicine, 21.
Responsibility: edited by William S.M. Wold.

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This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them...a very comprehensive methods book that will be a laboratory standard for years to come. - Read more...

 
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