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Adhesion protein protocols

Author: Elisabetta Dejana; Monica Corada
Publisher: Totowa, N.J. : Humana Press, ©1999.
Series: Methods in molecular biology (Clifton, N.J.), v. 96.
Edition/Format:   Print book : EnglishView all editions and formats
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Genre/Form: Aufsatzsammlung
Laboratory manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Elisabetta Dejana; Monica Corada
ISBN: 0896034178 9780896034174
OCLC Number: 40199991
Description: xi, 229 pages : illustrations ; 24 cm.
Contents: Monoclonal antibodies specific for leukocyte adhesion molecules : selective protocols of immunization and screening assays for generation of blocking, activating and activation reporter antibodies / Carlos Cabañas, Francisco Sánchez-Madrid --
Epitope mapping / Horace M. DeLisser --
Sequencing of antibodies / Annie Jarrin, Annie Andrieux --
Purification through affinity chromatography and microsequencing / Cecilia Garlanda --
Functional analysis of cell adhesion molecules / David L. Simmons --
Application of polymerase chain reaction for the discovery of new adhesion molecule family members / Amy S. Woodard, Lucia R. Languino --
Construction and purification of adhesion molecule immunoglobulin chimeric proteins / Thomas Moll, Dietmar Vestweber --
Immunofluorescence of cultured cells / Pier Carlo Marchisio, Livio Trusolino --
Detection of adhesion molecules by immunohistochemistry on human and murine tissue sections / Antonella Stoppacciaro, Luigi P. Ruco --
In situ hybridization with RNA probes / Georg Breier --
Purification of fibronectin from human plasma / Saverio Francesco Retta, Piercarlo Ferraris, Guido Tarone --
Adhesion to matrix proteins / Saverio Francesco Retta, Massimo Ternullo, Guido Tarone --
Leukocyte-endothelial monolayer adhesion assay (static conditions) / Jeanne-Marie Kiely, Francis W. Luscinskas, Michael A. Gimbrone --
Cell-cell adhesive interactions in an in vitro flow chamber / Douglas J. Goetz, Daniel M. Greif, Jian Shen, Francis W. Luscinskas --
Adhesion of tumor cells to endothelium under static conditions / Renato G. Chiarivi, Raffaella Giavazzi --
Adhesion of tumor cells under flow / Andrea Remuzzi, Raffaella Giavazzi --
Platelet adhesion to the subendothelium under flow / Philip G. Groot, Martin J. IJsseldijk, Jan J. Sixma --
Leukocyte transmigration through vascular endothelium : an in vitro method / Paola Allavena, Aldo Maschio --
Cell migration into a wounded area in vitro / Maria Grazia Lampugnani --
Determination of the endothelial barrier function in vitro / Geerten P. Nieuw Amerongen, Victor W. Hinsbergh --
Induction of intracellular signals through binding of adhesive molecules : activation of p125FAK tyrosine phosphorylation / Mascia Venturino, Cristina Olivo, Guido Tarone, Paola Defilippi --
Induction of Ca² flux by adhesion molecules in lymphocytes / Ruggero Maria, Gabriella Palmieri, Angela Santoni --
Integrin-mediated stimulation of tyrosine phosphorylation in lymphoid cells / Angela Gismondi, Angela Santoni --
The identification of signaling molecules by the yeast two-hybrid system / Stefano Biffo, Pier Carlo Marchisio.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 96.
Responsibility: edited by Elisabetta Dejana and Monica Corada.

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