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Advances in Understanding the Biology of Halophilic Microorganisms.

Author: Russell H Vreeland
Publisher: Dordrecht : Springer Netherlands : Imprint: Springer, 2012.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This book is designed to be a long term career reference. The chapters present modern procedures. This is a how-to-book with a difference. These chapters: - reveal the background information about working with salt loving organisms, - are loaded with information about how experiments are conducted under high salt, - provide information about analyses that work under these conditions and those that may not, - present  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Russell H Vreeland
ISBN: 9400798830 9789400798830 9400755384 9789400755383 9789400755390 9400755392
OCLC Number: 990670055
Language Note: English.
Notes: Non-Enzymatic Shielding Mechanisms.
Description: 1 online resource (244 pages)
Contents: Chapter 1Approaches Toward the Study of HalophilicMicroorganisms in Their Natural Environments:Who Are They and What Are They Doing?; Introduction; Characterization of the Microbial Communities in Hypersaline Brines-Qualitative and Quantitative Approaches; General Considerations; Use of Specific Biomarkers in Ecological Studies in Hypersaline Environments; Molecular Methods; Approaches Toward the Study of the In Situ Activities of Halophilic Microbial Communities; Protozoa, Viruses and Other Factors Controlling the Abundance of Halophilic Microorganisms; Final Comments; References. Chapter 2Media and Conditions for the Growth ofHalophilic and Halotolerant Bacteria andArchaeaIntroduction; Growth Media; General Comments; Media Composition; Salinity; Magnesium; Potassium; Sulfur, Phosphate, and Nitrogen; Minor and Trace Salts; Organic Components; Other Components; Specialty Media; Environmental Conditions; Other Salinophiles; Concluding Remarks; References; Chapter 3Taxonomy of Halophilic Archaea and Bacteria; Introduction; Taxonomy of Halophilic Archaea; Taxonomy of Halophilic Bacteria; Methodology for the Characterization of Halophiles; Phylogenetic Analysis. Phenotypic CharacteristicsChemotaxonomic Characteristics; Cellular Fatty Acids; Polar Lipids; Respiratory Quinone Systems; Peptidoglycan Structure; Genotypic Characteristics; DNA Base Composition; DNA-DNA Hybridization; References; Chapter 4Halophilic Viruses; Introduction; Ecological Studies; Highlights of In-depth Halophage Isolate Studies; Methods and Instrumentation; Future Directions; References; Chapter 5Microorganisms in Evaporites: Review ofModern Geomicrobiology; Introduction. Sedimentology and Microscopy of Evaporites and Fluid Inclusions: Syndepositional (Primary) Versus Burial (Secondary) Origin and Interpretation of PaleoenvironmentsFluid Inclusions in Halite and Gypsum; Brine Evolution and Secular Variations in the Major Ion Chemistry of Seawater; Fluid Inclusion Microthermetry: Paleobrine Temperatures; The Importance of Microscopy; Microbiological Considerations; Conclusions; References; Chapter 6Searching for Microbes and DNAin Ancient Halite; Introduction; Historical Perspective; Early Samples; Sterilization; Physical Laboratory; Sampling Parameters. Criteria for Site SelectionAge and Stability of Formations; Laboratory Configuration; Basic Techniques; Sample Considerations; Sterilization; What Makes a Discovery Believable?; Future Advances; References; Chapter 7 DNA Replication and Repair in Halophiles; Introduction; DNA Replication in Halophiles; Replication Origins; Origin Recognition Proteins; Helicases; Single-strand DNA Binding; DNA Replicative Polymerases and Sliding Clamps; Okazaki Fragments Processing; DNA Repair Pathways in Halophiles; DNA Damage Avoidance Strategies in Halophiles; The Role of Pigments.

Abstract:

This book is designed to be a long term career reference. The chapters present modern procedures. This is a how-to-book with a difference. These chapters: - reveal the background information about working with salt loving organisms, - are loaded with information about how experiments are conducted under high salt, - provide information about analyses that work under these conditions and those that may not, - present a wide range of details from laboratory designs to equipment used and even to simple anecdotal hints that can only come from experience. Microbiological training focuses largely on.

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