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Advancing methods for biomolecular crystallography

Verfasser/in: Randy J Read; Alexandre G Urzhumtsev; Vladimir Y Lunin
Verlag: Dordrecht : Springer, ©2013.
Serien: NATO science for peace and security series., Series A,, Chemistry and biology.
Ausgabe/Format   E-Book : Dokument : Tagungsband : EnglischAlle Ausgaben und Formate anzeigen
Datenbank:WorldCat
Zusammenfassung:
This work presents a snapshot of the state of the art of modern biomolecular crystallography, from crystallisation through structure determination and even interactive presentation on the web. Methods driving the latest automated structure determination pipelines are explained, as well as how to deal with problems such as crystal pathologies that still demand expert analysis. These methods are illustrated through  Weiterlesen…
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Gattung/Form: Electronic books
Conference proceedings
Congresses
Medientyp: Tagungsband, Dokument, Internetquelle
Dokumenttyp: Internet-Ressource, Computer-Datei
Alle Autoren: Randy J Read; Alexandre G Urzhumtsev; Vladimir Y Lunin
ISBN: 9789400762329 9400762321
OCLC-Nummer: 839438584
Anmerkungen: "Proceedings of the NATO Advanced Study Institute on Present and Future Methods of Biomolecular Crystallography: the Structural Path to Defence Against CBRN Agents, Erice Italy, 1-10 June 2012."--t.p. verso.
International conference proceedings.
Beschreibung: 1 online resource.
Inhalt: The Zen of Model Anomalies - Correct Most of Them. Treasure the Meaningful Valid Few. Live Serenely with the Rest! / Jane S. Richardson, David C. Richardson --
Total Chemical Protein Synthesis for the Determination of Novel X-ray Structures by Racemic Protein Crystallography / Kalyaneswar Mandal --
Crystal Pathologies / Todd O. Yeates --
Crystallizing Membrane Proteins for Structure-Function Studies Using Lipidic Mesophases / Martin Caffrey --
Searching for Needles in Haystacks: Automation and the Task of Crystal Structure Determination / Seán McSweeney --
Data Processing: How Good Are My Data Really? / Kay Diederichs, P. Andrew Karplus --
Radiation Damage in Macromolecular Crystallography: What Is It and Why Do We Care? / Elspeth F. Garman --
Elemental Analysis of Proteins by Proton Induced X-ray Emission (microPIXE) / Elspeth F. Garman, Oliver B. Zeldin --
X-rays-Induced Cooperative Atomic Movement in a Protein Crystal / Tatiana Petrova, Vladimir Y. Lunin --
Everything Happens at Once - Deconvolving Systematic Effects in X-ray Data Processing / Dominika Borek, Zbyszek Otwinowski --
Extending the Reach of Molecular Replacement / Randy J. Read, Airlie J. McCoy --
Phasing Through Location of Small Fragments and Density Modification with ARCIMBOLDO / Isabel Usón, Claudia Millán, Massimo Sammito --
SAD/MAD Phasing / Zbigniew Dauter --
Macromolecular Phasing: Solving the Substructure / Tim Grüne --
Advanced Applications of Shelxd and Shelxe / Tim Grüne --
Use of a Weak Anomalous Signal for Phasing in Protein Crystallography: Reflection from Personal Experience / Felix Frolow --
Ab Initio Low Resolution Phasing / Vladimir Y. Lunin, Natalia L. Lunina --
Model-Building and Reduction of Model Bias in Electron Density Maps / Thomas C. Terwilliger --
Using Coot to Model Protein and Ligand Structures Using X-ray data / Paul Emsley --
Crystallographic Structure Refinement in a Nutshell / Pavel V. Afonine, Paul D. Adams --
Crystallographic Maps and Models at Low and at Subatomic Resolutions / Alexandre G. Urzhumtsev, Pavel V. Afonine --
Recent Advances in Low Resolution Refinement Tools in REFMAC5 / Robert A. Nicholls, Fei Long --
High Resolution Macromolecular Crystallography / Mariusz Jaskolski --
Publishing in Proteopedia: The Guide / Jaime Prilusky, Wayne Decatur, Eric Martz --
Proteolysis, Complex Formation and Conformational Changes Drive the Complement Pathways / Piet Gros, Federico Forneris --
Monoamine Oxidase Inhibitors: Diverse and Surprising Chemistry with Expanding Pharmacological Potential / Claudia Binda, Dale E. Edmondson --
Structure of the Eukaryotic Ribosome: Tips and Tricks / Sergey Melnikov --
Neutron Protein Crystallography. How to Proceed the Experiments to Obtain the Structural Information of Hydrogen, Protons and Hydration in Bio-macromolecules / Nobuo Niimura --
Coherent Diffraction and Holographic Imaging of Individual Biomolecules Using Low-Energy Electrons / Tatiana Latychevskaia --
Structure Analysis of Biological Macromolecules by Small-Angle X-ray Scattering / Dmitri I. Svergun --
Protein Structure Modeling with Rosetta: Case Studies in Structure Prediction and Enzyme Repurposing / Frank DiMaio.
Serientitel: NATO science for peace and security series., Series A,, Chemistry and biology.
Verfasserangabe: edited by Randy Read, Alexandre G. Urzhumtsev, and Vladimir Y. Lunin.
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Abstract:

This work presents a snapshot of the state of the art of modern biomolecular crystallography, from crystallisation through structure determination and even interactive presentation on the web. Methods driving the latest automated structure determination pipelines are explained, as well as how to deal with problems such as crystal pathologies that still demand expert analysis. These methods are illustrated through their application to problems of great biological interest, such as the molecular machinery underlying the complement pathway, the mechanism of action of monoamine oxidase inhibitors, and the structure of the eukaryotic ribosome. Complementary approaches, such as neutron diffraction, small angle X-ray scattering, coherent diffraction and computational modelling, are also explored.

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