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Aerobic Mineralisation in Surface Water - Simulation Biodegradation Test

Author: Organisation for Economic Co-operation and Development.
Publisher: [Paris] : OECD Pub., 2004.
Series: OECD Guidelines for the Testing of Chemicals., Section 3,, Degradation and Accumulation.
Edition/Format:   eBook : International government publication : EnglishView all editions and formats
Summary:
The purpose of this test is to measure the time course of biodegradation of a test substance at low concentration in aerobic natural water and to quantify the observations in the form of kinetic rate expressions. The test is performed in batch by "pelagic test" or "suspended sediment test" to simulate a water body with suspended solids or re-suspended sediment. The test flasks are incubated in darkness at an  Read more...
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Material Type: Government publication, International government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Organisation for Economic Co-operation and Development.
ISBN: 9789264070547 9264070540
OCLC Number: 704444750
Description: 1 online resource (21 pages) : illustrations.
Series Title: OECD Guidelines for the Testing of Chemicals., Section 3,, Degradation and Accumulation.
Other Titles: 309, adopted 13 April 2004
Responsibility: Organisation for Economic Co-operation and Development.

Abstract:

The purpose of this test is to measure the time course of biodegradation of a test substance at low concentration in aerobic natural water and to quantify the observations in the form of kinetic rate expressions. The test is performed in batch by "pelagic test" or "suspended sediment test" to simulate a water body with suspended solids or re-suspended sediment. The test flasks are incubated in darkness at an environmental temperature under aerobic conditions and agitation during 60 days normally. At least two different concentrations of test substance (non-volatile or slightly volatile organic) should be used. The maximum test concentration should be less than 100L (biodegradation following first order kinetics) and the lowest test concentration preferably in the range of 2039L. Two subsamples should be withdrawn from each test flask at each sampling time. Degradation is followed at appropriate time intervals, by measuring either the residual 14C or the residual concentration of test substance. The total mineralisation and the primary biodegradation are determined by a different 14C labeling part of the molecule. Periodic measurements of pH and oxygen concentration in the test system must be conducted.

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