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Affinity chromatography : methods and protocols.
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Affinity chromatography : methods and protocols.

Author: M Zachariou; Pascal Bailon
Publisher: Totowa, N.J. : Humana ; London : Springer [distributor], 2007.
Series: Methods in molecular biology, 421.
Edition/Format:   Book : English : 2nd ed.View all editions and formats
Summary:

This expanded and updated volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic  Read more...

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Document Type: Book
All Authors / Contributors: M Zachariou; Pascal Bailon
ISBN: 9781588296597 1588296598 9781597455824 1597455822
OCLC Number: 166315293
Notes: Previous ed.: published as by Pascal Bailon, George K. Ehrlich. 2000.
Description: xii, 343 p. : ill.
Contents: PROPOSED TABLE OF CONTENTS 1 Affinity Chromatography: History, Perspectives, Limitations and Prospects Ana Ceci-lia A. Roque and Christopher R. Lowe VARIOUS MODES OF AFFINITY CHROMATOGRAPHY 2 Immobilized Metal Ion Affinity Chromatography of Native Proteins Adam Charlton and Michael Zachariou 3 Affinity Precipitation of Proteins Using Metal Chelates Ashok Kumar, Igor Yu. Galaev and Bo Mattiasson 4 Immunoaffinity Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 5 Dye-ligand Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 6 Purification of Proteins Using Displacement Chromatography Nihal Tugcu AFFINITY CHROMATOGRAPHY USING PURIFICATION TAGS 7 Rationally Designed Ligands for use in Affinity Chromatography: an Artificial Protein L Ana Ceci-lia A. Roque and Christopher Lowe 8 Phage display of Peptides in Ligand Selection for use in Affinity Chromatography Joanne L Casey, Andrew M. Coley and Michael Foley 9 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Gareth M. Forde 10 Immobilized Metal Ion Affinity Chromatography of Histidine-tagged fusion proteins Adam Charlton and Michael Zachariou 11 Methods for the Purification of HQ-Tagged Proteins Beckky Godat, Laurie Engel, Natalie Betz and Tonny M. Johnson 12 Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis-Refolding Methods. Leonard K. Pattenden and Walter G. Thomas 13 Methods for the Detection of Protein-Protein and Protein-DNA Interactions using Halo-Tag(t) Marjeta Urh, Danette Hartzell, Jacqui Mendez and Keith Wood 14 Site-specific Cleavage of Fusion Proteins Adam Charlton 15 The Use of TAGzyme for the efficient Removal of N-terminal His-tags Jose Arnau, Conni Lauritzen, Gitte E. Petersen and John Pedersen VARIOUS APPLICATIONS OF AFFINITY CHROMATOGRAPHY. 16 Affinity Processing of Cell-containing Feeds Using Monolithic Macroporous Hydrogels, Cryogels Igor Yu. Galaev and Bo Mattiasson 17 Monolithic Bioreactors for Macromolecules Mojca Ben_ina, Katja Ben_ina, Ale_ Podgornik and Ale_ _trancar 18 Plasmid DNA Purification via the Use of a Dual Affinity Protein Gareth M. Forde 19 Affinity Chromatography of Phosphorylated Proteins Grigoriy S. Tchaga 20 Protein Separation Using Phospholipid Chromatography Tzong-Hsien Lee and Marie-Isabel Aguilar 21 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Neils H.H. Heegaard, Christian Schou and Jesper A stergaard
Series Title: Methods in molecular biology, 421.

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From the reviews of the second edition: "The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. ! recommends this book as a Read more...

 
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