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Affinity chromatography : methods and protocols

Author: Pascal Bailon
Publisher: Totowa, NJ : Humana Press, ©2000.
Series: Methods in molecular biology (Clifton, N.J.), v. 147.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
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Genre/Form: Laboratory Manuals
Fulltext
Internet Resource
Index not Present
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Affinity chromatography.
Totowa, NJ : Humana Press, ©2000
(DLC) 99053967
(OCoLC)42708076
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Pascal Bailon
ISBN: 9781603272612 1603272615 1592590411 9781592590414
OCLC Number: 227334564
Description: 1 online resource (x, 230 pages) : illustrations.
Contents: An overview of affinity chromatography --
Weak affinity chromatography --
Fluidized-bed recepter-affinity chromatography --
Site-specific immobilization of antibodies to protein G-derivatized solid supports --
Affinity purification of monoclonal antibiodies --
Proetin A mimetic (PAM) affinity chromatography: immunoglobulins purification --
Periodate oxidation of antibodies for site-selective immobolization in immunoaffinity chromatography --
Immunoaffinity chromatography --
Affinity partitioning of proteins --
Boronate affinity chromatography --
Dye-ligand affinity chromatography for protein separation and purification --
DNA affinity chromatography --
Affinity chromatography of pyrogens --
Western cross blot --
Affinity perfusion chromatography --
Phge display technology: affinity selectin by biopanning --
Phage display technology: identification of peptides as model ligands for affinity chromatography.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 147.
Responsibility: edited by Pascal Bailon [and others].

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Affinity chromatography is a powerful tool, with exquisite specificity, for the purification of biomolecules. This comprehensive collection of detailed affinity chromatography methods provides all the information necessary to use these powerful techniques successfully in the laboratory and in real world situations. The collection includes classic, current, and state-of-the-art methods, all written by experts who have perfected them in their laboratories. The techniques range from small molecule to protein ligands, and to supra molecular structures in phage display.

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