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Affinity chromatography : methods and protocols

Author: Senta Reichelt
Publisher: New York : Humana Press : Springer, [2015] ©2015
Series: Methods in molecular biology (Clifton, N.J.), v. 1286.
Edition/Format:   eBook : Document : English : Third editionView all editions and formats
Summary:
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, OCLCbrA0 molecular modelling, OCLCbrA0 and the study of ligand-target interactions. Written in the successful Methods in  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Affinity chromatography.
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Senta Reichelt
ISBN: 9781493924479 1493924478 149392446X 9781493924462
OCLC Number: 904558121
Notes: Revised edition of: Affinity chromatography / edited by Michael Zachariou, c2008.
Description: 1 online resource (xiii, 341 pages) : illustrations (some color).
Contents: Affinity chromatography : a historical perspective / David S. Hage and Ryan Matsuda --
Protein purification by aminosquarylium cyanine dye-affinity chromatography / Vania C. Graca [and others] --
One-step purification of phosphinothricin acetyltransferase using reactive dye-affinity chromatography / Cunxi Wang [and others] --
Antibody purification from human plasma by metal-chelated affinity membranes. / Handan Yavuz [and others] --
Specific recognition of supercoiled plasmid DNA by affinity chromatography using a synthetic aromatic ligand / Catarina Caramelo-Nunes and Candida T. Tomaz --
Cell affinity separations on microfluidic devices / Yan Gao, Wenjie Li, Ye Zhang, and Dimitri Pappas --
Aptamer-modified magnetic beads in affinity separation of proteins / Guohong Zhu and Johanna-Gabriela Walter --
Strep-tag system for one-step affinity purification of proteins from mammalian cell culture / Thomas Schmidt and Arne Skerra --
Robotic high-throughput purification of affinity-tagged recombinant proteins / Simone C. Wiesler and Robert O.J. Weinzierl --
Macroporous silica particles derivatized for enhanced lectin affinity enrichment of glycoproteins / Benjamin F. Mann --
Immobilized magnetic beads-based multi-target affinity selection coupled with HPLC-MS for screening active compounds from traditional chinese medicine and natural products / Yaqi Chen, Zhui Chen, and Yi Wang --
Mixed-bed affinity chromatography : principles and methods / Egisto Boschetti and Pier Giorgio Righetti --
Preparation and characterization of fluorophenylboronic acid-functionalized affinity monolithic columns for the selective enrichment of cis-diol-containing biomolecules / Qianjin Li and Zhen Liu --
Introduction to macroporous cryogels / Senta Reichelt --
Cryogels with affinity ligands as tools in protein purification / Solmaz Hajizadeh and Bo Mattiasson --
Direct capture of His6-tagged proteins using megaporous cryogels developed for metal-ion affinity chromatography / Naveen Kumar Singh [and others] --
Ni(II) chelated IDA functionalized poly(HEMA-GMA) cryogels for urease adsorption / Murat Uygun --
Novel chromatographic media : histidine-containing composite cryogels for HIgG separation from human serum / Gozde Baydemir and Mehmet Odabasi --
Molecularly imprinted cryogels for human serum albumin depletion / Muge Andac [and others] --
Interpenetrating polymer network composite cryogels with tailored porous morphology and sorption properties / Ecaterina Stela Dragan and Maria Valentina Dinu --
Analysis of drug-protein interactions by high-performance affinity chromatography : interactions of sulfonylurea drugs with normal and glycated human serum albumin / Ryan Matsuda [and others] --
Accurate protein-peptide titration experiments by nuclear magnetic resonance using low-volume samples / Christian Kohler [and others] --
Characterization of the binding strengths between boronic acids and cis-diol-containing biomolecules by affinity capillary electrophoresis / Chenchen Lu and Zhen Liu --
Determination of the kinetic rate constant of cyclodextrin supramolecular systems by high-performance affinity chromatography / Jiwen Zhang [and others] --
Molecular modeling of the affinity chromatography of monoclonal antibodies / Matteo Paloni and Carlo Cavallotti.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1286.
Responsibility: edited by Senta Reichelt.

Abstract:

The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols.  Read more...

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