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Allergy methods and protocols

Author: Meinir G Jones; Penny Lympany
Publisher: Totowa, NJ : Humana Press, ©2008.
Series: Methods in molecular medicine, 138.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Protocols a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Allergy methods and protocols.
Totowa, N.J. : Humana Press, ©2008
(DLC) 2007932488
(OCoLC)228168447
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Meinir G Jones; Penny Lympany
ISBN: 1597453668 9781597453660
OCLC Number: 213262222
Language Note: English.
Description: 1 online resource (xi, 352 pages) : illustrations.
Contents: Understanding of the molecular mechanisms of allergy / Meinir Jones --
T cell : primary culture from peripheral blood / Monika Raulf-Heimsoth --
Production of T-cell lines / Helga Kahlert --
Production of human T-cell clones / Adrienne Verhoef --
Mapping of human T-cell epitopes of allergens / Thomas Zeiler and Tuomas Virtanen --
Determining MHC restriction of T-cell responses / Mark Larché --
Short-term culture of CD8 cells and intracellular cytokine staining / Beejal Vyas and Alistair Noble --
Isolation, flow cytometric analysis, and suppression assay of CD4+ CD25+ T-regulatory cells / Hayley Jeal --
Monocyte-derived dendritic cells as antigen-presenting cells in T-cell proliferation and cytokine production / Sun-sang J. Sung --
Ultrasensitive ELISA for measurement of human cytokine responses in primary culture / William P. Stefura [and others] --
Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses / Monique Stinson [and others] --
Standardization of allergen extracts / Jørgen Nedergaard Larsen and Sten Dreborg --
Immunoelectrophoresis for the characterization of allergen extracts / Gitte Nordskov Hansen and Jørgen Nedergaard Larsen --
Conjugation of haptens / Ranulfo Lemus and Meryl H. Karol --
Monoclonal antibodies / Helga Kahlert and Oliver Cromwell --
Purification of antibodies / Per H. Larsson --
Collection of air samples to quantify exposure to airborne allergens / Susan Gordon --
Assay of air sample eluates / Anne Renström and Susan Gordon --
The halogen assay : a new technique for measuring airborne allergen / Euan Tovey [and others] --
Measurement of specific IgG anti-Fel d 1 antibodies / Meinir G. Jones --
The facilitated antigen binding (FAB) assay : a protocol to measure allergen-specific inhibitory antibody activity / James N. Francis --
Microscopic identification and purity determination of pollen grains / Magdalena Rahl --
Biopanning for the characterization of allergen mimotopes / Isabella Pali-Schöll and Erika Jensen-Jarolim --
Identification of mast cells and mast cell subpopulations / Mark Buckley and Andrew F. Walls --
Purification and characterization of mast cell tryptase and chymase from human tissues / Alan R. McEuen and Andrew F. Walls --
Experimental activation of mast cells and their pharmacological modulation / Shaoheng He and Andrew F. Walls --
In situ hybridization / Kayhan T. Nouri-Aria.
Series Title: Methods in molecular medicine, 138.
Responsibility: edited by Meinir G. Jones and Penny Lympany.

Abstract:

Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols.  Read more...

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From the reviews:"The purpose is to provide a comprehensive and readily accessible guide to techniques used in the investigation of allergic diseases. ... Students embarking on an investigative Read more...

 
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