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Amphibian metamorphosis assay

Author: Organisation for Economic Co-operation and Development.
Publisher: [Paris] : OECD Publishing, 2009.
Series: OECD Guidelines for the Testing of Chemicals., Section 2,, Effects on Biotic Systems.
Edition/Format:   eBook : International government publication : EnglishView all editions and formats
Summary:
This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the species Xenopus laevis, which is recommended for use in the Guideline. The assay uses three test chemical concentrations and the necessary controls, including a carrier control if necessary. The assay  Read more...
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Details

Material Type: Government publication, International government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Organisation for Economic Co-operation and Development.
ISBN: 9789264076242 9264076247
OCLC Number: 704444712
Description: 1 online resource (33 pages).
Series Title: OECD Guidelines for the Testing of Chemicals., Section 2,, Effects on Biotic Systems.
Other Titles: 231, adopted 7 September 2009
Responsibility: Organisation for Economic Co-operation and Development.

Abstract:

This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the species Xenopus laevis, which is recommended for use in the Guideline. The assay uses three test chemical concentrations and the necessary controls, including a carrier control if necessary. The assay starts with tadpoles at the development stage 51 on the Nieuwkoop and Faber scale and is extended for a duration of 21 days. Four replicate test vessels are used for each treatment level and control(s). After 7 days of exposure, a sub-set of tadpoles from each treatment level is sampled for the measurement of the length of the hind-limb. At termination of 21-day exposure period, developmental stage, snout-to-vent length and hind limb length are measured on all remaining tadpoles. A sub-set of tadpoles from each treatment level is fixed (whole-body or dissected) for histopathology of the thyroid gland.

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