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Animal cell culture : essential methods

Author: John Davis
Publisher: Hoboken, NJ : John Wiley & Sons Inc., 2011.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then  Read more...

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Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: John Davis
ISBN: 9780470666586 0470666587 9780470669815 0470669810
OCLC Number: 664450733
Description: xxvii, 346 pages : illustrations ; 25 cm
Contents: The cell culture laboratory / Sue Clarke and Janette Dillon --
Sterilization / Peter L. Roberts --
Microscopy of living cells / Colin Gray and Daniel Zicha --
Basic techniques and media, the maintenance of cell lines, and safety / John M. Davis --
Development and optimization of serum- and protein-free culture media / Stephen F. Gorfien and David W. Jayme --
Cryopreservation and banking of cell lines / Glyn N. Stacey, Ross Hawkins and Roland A. Fleck --
Primary culture of specific cell types and the establishment of cell lines / Kee Woei Ng [and others] --
Cloning / John Clarke, Alison Porter and John M. Davis --
The quality control of animal cell lines and the prevention, detection and cure of contamination / Peter Thraves and Cathy Rowe --
Systems for cell culture scale-up / Jennifer Halsall and John M. Davis --
Good laboratory practice in the cell culture laboratory / Barbara Orton.
Responsibility: editor, John M. Davis.

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"It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques." (Doody's, 13 January 2012)

 
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