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Animal endo-SiRNAs : methods and protocols

Author: Andreas Werner
Publisher: New York, NY : Humana Press, 2014.
Series: Methods in molecular biology (Clifton, N.J.), 1173.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Animal endo-SiRNAs.
(DLC) 2014941237
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Andreas Werner
ISBN: 9781493909315 1493909312 1493909304 9781493909308
OCLC Number: 881713953
Description: 1 online resource (x, 182 pages) : illustrations (some color).
Contents: Targeted small noncoding rna-directed gene activation in human cells --
Isolation of chromatoid bodies from mouse testis as a rich source of short RNAs --
Generation of endo-siRNAs in xenopus laevis oocytes --
Analysis of endo-siRNAs in drosophila --
Methods for studying the biological consequences of endo-siRNA deficiency in drosophila melanogaster --
Small RNA library cloning procedure for deep sequencing of specific endogenous siRNA classes in caenorhabditis elegans --
Assays for direct and indirect effects of c. Elegans endo-siRNAs --
Extraction and nonradioactive detection of small RNA molecules --
P19-mediated enrichment and detection of siRNAs --
Detection of small noncoding RNAs by in situ hybridization using probes of 2'-o-methyl RNA + LNA --
Enhanced detection of small RNAs using a nonradioactive approach --
Computing siRNA and piRNA overlap signatures --
Isolation of small interfering RNAs using viral suppressors of RNA interference --
Computational analysis, biochemical purification, and detection of tRNA-derived small RNA fragments --
Differential DNA methylation patterns in endo-siRNAs mediated silencing of LINE-1 retrotransposons.
Series Title: Methods in molecular biology (Clifton, N.J.), 1173.
Responsibility: edited by Andreas Werner.

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