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Antibiotic resistance protocols

Author: S H Gillespie; Timothy D McHugh
Publisher: New York : Humana Press, ©2010.
Series: Methods in molecular biology (Clifton, N.J.), v. 642.; Springer protocols (Series)
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness  Read more...

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Genre/Form: Laboratory Manuals
Laboratory manuals
Additional Physical Format: Online version:
Antibiotic resistance protocols.
New York : Humana Press, ©2010
(OCoLC)741516393
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: S H Gillespie; Timothy D McHugh
ISBN: 9781603272780 160327278X 9781603272797 1603272798
OCLC Number: 506251779
Description: x, 227 pages : illustrations ; 24 cm.
Contents: The RM test for determining methicillin-resistant Staphylococcus aureus lineages / J.A. Lindsay and J.M. Sung --
Unravelling the ecology of antibiotic resistant bacteria in the nasopharynx / B.M. Charalambous and N.M. Oriyo --
Methods to determine antibiotic resistance gene silencing / V.I. Enne and P.M. Bennett --
Studying gene induction of glycopeptide resistance using gene swapping / H.J. Hong --
Real-time PCR methods to study expression of genes related to hypermutability / D.M. O'Sullivan --
Use of DNA arrays to study transcriptional responses to antimycobacterial compounds / S.J. Waddell and P.D. Butcher --
Assays of sensitivity of antibiotic-resistant bacteria to hydrogen peroxide and measurement of catalase activity / M. Macvanin and D. Hughes --
Measurements of heme levels and respiration rate in Salmonella enterica serovar typhimurium / D. Hughes and M. Macvanin --
Methods to determine fitness in bacteria / C.F. Pope, T.D. McHugh, and S.H. Gillespie. Application of continuous culture for measuring the effect of environmental stress on mutation frequency in Mycobacterium tuberculosis / J. Bacon, K.A. Hatch, and J. Allnutt --
Identification of efflux-mediated multi-drug resistance in bacterial clinical isolates by two simple methods / M. Martins [and others] --
Evaluation of efflux activity of bacteria by a semi-automated fluorometric system / M. Viveiros [and others] --
Measuring the activity of active efflux in gram-negative bacteria / M.A. Webber and N.G. Coldham --
Rapid characterization of beta-lactamases by multiplex PCR / N. Woodford --
Rapid methods for testing inhibitors of mycobacterial growth / D. Evangelopoulos and S. Bhakta --
Use of biofilm model systems to study antimicrobial susceptibility / J. Pratten and D. Ready --
Rapid analysis of resistant mutant genotypes using pyrosequencing / C. Arnold.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 642.; Springer protocols (Series)
Responsibility: edited by Stephen H. Gillespie, Timothy D. McHugh.
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From the reviews:"...an excellent, well-produced book, full of protocols on a range of modern molecular techniques, applicable not only to research on antibiotics resistance, but to general Read more...

 
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