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Antibody engineering : methods and protocols

Author: Benny K C Lo
Publisher: Totowa, N.J. : Humana Press, ©2004.
Series: Methods in molecular biology (Clifton, N.J.), v. 248.
Edition/Format:   Print book : EnglishView all editions and formats
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Despite this setback, advances in recombinant DNA techniques over the last 15-20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Manuels de laboratoire
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Benny K C Lo
ISBN: 1588290921 9781588290922 1592596665 9781592596669
OCLC Number: 53051674
Description: xiv, 561 pages : illustrations ; 24 cm.
Contents: pt. 1. Antibody sequence and structure --
Internet resources for the antibody engineer / Benny K.C. Lo --
The Kabat database and a bioinformatics example / George Johnson --
IMGT, the International ImMunoGeneTics Information Sysmtem, http://imgt.cines.fr / Marie-Paule Lefranc --
Antibody variable regions: toward a unified modeling method / Nicholas Whitelegg --
Studying antibody conformations by ultracentrifugation and hydrodynamic modeling / Stephen E. Harding --
pt. 2. Antibody-lead generation --
PCR cloning of human immunoglobulin genes / James D. Marks --
Antibody humanization by CDR grafting / Benny K.C. Lo --
Selection of human antibodies from phage display libraries / James D. Marks --
Production of human single-chain antibodies by ribosome display / Mingyue He --
Production of human antibodies from transgenic mice / C. Geoffrey Davis --
Selection of internalizing antibodies for drug delivery / James D. Marks --
Engineering multivalent antibody fragments for in vivo targeting / Anna M. Wu --
Production of recombinant bispecific antibodies / Roland E. Kontermann --
pt. 3. Antibody expression and optimization --
Expression and isolation of recombinant antibody fragments in E. coli / Keith A. Charlton --
Expression of recombinant antibodies in mammalian cell lines / Paul J. Yazaki --
Human antibody production using insect-cell expression systems / Mary C. Guttieri --
Antibody production in transgenic plants / Eva Stoger --
Directed mutagenesis of antibody variable domains / Kevin Brady --
Antibody affinity maturation by chain shuffling / James D. Marks --
Antibody affinity maturation by random mutagenesis / Ikuo Fujii --
Developing a minimally immunogenic humanized antibody by SDR grafting / Syed V.S. Kashmiri --
pt. 4. Antibody characterization and novel applications --
Antibody purification by column chromatography / Andrea Murray --
Affinity measurement using surface plasmon resonance / Robert Karlsson --
Kinetic exclusion assays to study high-affinity binding interactions in homogenous solutions / Robert C. Blake II --
Characterization of antibody-antigen interactions by fluorescence spectroscopy / Sotiris Missailidis --
Antibody epitope mapping using arrays of synthetic peptides / Ulrich Reineke --
Mapping antibody: antigen interactions by mass spectrometry and bioinformatics / Alexandra Huhalov --
Radiometal labeling of antibodies and antibody fragments for imaging and therapy / Ilse Novak-Hofer --
Production and characterization of anti-cocaine catalytic antibodies / Paloma de Prada --
Recombinant immunotoxins in the treatment of cancer / Ira Pastan --
Antibodies in proteomics / Andrew R.M. Bradbury --
Targeting of antibodies using aptamers / Sotiris Missailidis.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 248.
Responsibility: edited by Benny K.C. Lo.
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"...as the book provides as comprehensive collection of clear, detailed methods it is very likely to prove its worth as a laboratory reference." - Immunology News"...a valuable addition to in-lab Read more...

 
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