skip to content
Antibody engineering. Volume 1 Preview this item
ClosePreview this item
Checking...

Antibody engineering. Volume 1

Author: Roland Kontermann; Stefan Dübel
Publisher: Berlin ; London : Springer, 2010.
Edition/Format:   eBook : Document : English : 2nd edView all editions and formats
Summary:

This book is a toolbox of well-established basics and many exciting new techniques in antibody engineering, reflecting the latest hands-on work of key laboratories in this fast-moving field. The new  Read more...

Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

 

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Antibody engineering. Volume 1.
Berlin ; London : Springer, 2010
(OCoLC)477295483
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Roland Kontermann; Stefan Dübel
ISBN: 9783642011443 3642011446
OCLC Number: 630107729
Notes: Previous edition: published in one volume. 2001.
Description: 1 online resource (viii, 788 pages)
Contents: Part 1. Cloning of the antigen-binding site from hybridoma: Cloning of variable domains from mouse hybridoma by PCR --
Coning hybridoma cDNA by RACE --
Construction of scFv fragments from hybridoma or spleen cells by PCR assembly --
Part 2: Generation of antibody repertoires --
Mouse immune libraries for the generation of scFv fragments directed against human cell surface antigens --
Human antibody gene libraries --
Synthetic antibody Libraries --
Immune libraries from nonhuman primates (NHP) --
Generation of rabbit immune libraries --
Part III: Selection of antibody fragments from combinatorial libraries --
Immunotube selections --
Phage display and selection in microtitre plates --
Phage display and selections on biotinylated antigens --
Phage display and subtractive selection on cells --
Selection of phage antibody libraries for binding and internalization into mammalian cells --
Improving phage display throughput by using hyperphage, miniaturized titration and pVIII (g8p) ELISA --
yeast display and selections --
The generation of transgenic mice expressing human antibody repertoires --
Selection of antibody fragments by means of the gilter-sandwich colony screening assay --
Semi-automated magnetic bead-based antibody selection from phage display libraries --
Part IV: Engineering and production of immunoglobulins --
Aspects of isotype selection --
Generation of heavy and light chains (chimeric antibodies) --
Humanising antibodies by CDR grafting --
Humanization by resurfacing --
Human antibodies by guided selection --
In silico de-immunization --
Affinity maturation by chain shuffling and site directed mutagenesis --
Affinity maturation by random mutagenesis and phage display --
Engineering of the Fc Region for Improved PK (FcRn Interaction) --
Antibody-dependent Enzyme Prodrug Therapy (ADEPT) --
Production of recombinant human IgG antibodies in the baculovirus expression system --
Expression of IgA molecules in mammalian cells --
Part V: Antibody characterization --
Expression of complete antibodies in transgenic plants --
Expression of full length monoclonal antibodies (mAb) in algal chloroplast --
Expression of IgG Antibodies in mammalian cells --
Protein A/G chromatography --
Epitope analysis using synthetic peptide repertoires prepared by SPOT synthesis technology --
Epitope mapping by printed peptide libraries --
Antibody epitope mapping using yeast display --
Size exclusion chromatography --
Structural characterization of antibodies by mass spectrometry --
Antibody glycans characterization --
Affinity measurements by competition ELISA --
Anti-histidine antibodies as tools for reversible capturing of His-tagged fusion proteins for subsequent binding analysis --
Affinity measurements using quartz crystal microbalance (QCM) --
Affinity measurements with radiolabeled antibodies --
Neutralization tests --
Functional characterization of antibodies neutralizing soluble factors in vitro and in vivo --
Competitive ELISA --
Quantification of human IgG and related Fc fusion proteins by a human IgG/Fc capture ELISA --
Determination of Fc-mediated antibody-effector functions by chromium release assay --
Binding studies with flow cytometry --
Index.
Responsibility: Roland Kontermann, Stefan Dubel (eds.).

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/630107729> # Antibody engineering. Volume 1
    a schema:Book, schema:MediaObject, schema:CreativeWork ;
    library:oclcnum "630107729" ;
    library:placeOfPublication <http://dbpedia.org/resource/London> ; # London
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/gw> ;
    library:placeOfPublication <http://experiment.worldcat.org/entity/work/data/3944155247#Place/berlin> ; # Berlin
    schema:about <http://experiment.worldcat.org/entity/work/data/3944155247#Topic/dna_recombinant_immunology> ; # DNA, Recombinant--immunology
    schema:about <http://experiment.worldcat.org/entity/work/data/3944155247#Topic/sciences_de_la_vie> ; # Sciences de la vie
    schema:about <http://experiment.worldcat.org/entity/work/data/3944155247#Topic/antibody_formation_genetics> ; # Antibody Formation--genetics
    schema:about <http://experiment.worldcat.org/entity/work/data/3944155247#Topic/biomedecine> ; # Biomédecine
    schema:about <http://experiment.worldcat.org/entity/work/data/3944155247#Topic/antibodies_genetics> ; # Antibodies--genetics
    schema:about <http://id.worldcat.org/fast/1091483> ; # Recombinant antibodies
    schema:about <http://experiment.worldcat.org/entity/work/data/3944155247#Topic/genetic_engineering_methods> ; # Genetic Engineering--methods
    schema:about <http://id.loc.gov/authorities/subjects/sh99001981> ; # Recombinant antibodies
    schema:about <http://dewey.info/class/616.0798/e22/> ;
    schema:bookEdition "2nd ed." ;
    schema:bookFormat schema:EBook ;
    schema:contributor <http://viaf.org/viaf/57496664> ; # Roland Kontermann
    schema:contributor <http://viaf.org/viaf/64255356> ; # Stefan Dübel
    schema:datePublished "2010" ;
    schema:description "Part 1. Cloning of the antigen-binding site from hybridoma: Cloning of variable domains from mouse hybridoma by PCR -- Coning hybridoma cDNA by RACE -- Construction of scFv fragments from hybridoma or spleen cells by PCR assembly -- Part 2: Generation of antibody repertoires -- Mouse immune libraries for the generation of scFv fragments directed against human cell surface antigens -- Human antibody gene libraries -- Synthetic antibody Libraries -- Immune libraries from nonhuman primates (NHP) -- Generation of rabbit immune libraries -- Part III: Selection of antibody fragments from combinatorial libraries -- Immunotube selections -- Phage display and selection in microtitre plates -- Phage display and selections on biotinylated antigens -- Phage display and subtractive selection on cells -- Selection of phage antibody libraries for binding and internalization into mammalian cells -- Improving phage display throughput by using hyperphage, miniaturized titration and pVIII (g8p) ELISA -- yeast display and selections -- The generation of transgenic mice expressing human antibody repertoires -- Selection of antibody fragments by means of the gilter-sandwich colony screening assay -- Semi-automated magnetic bead-based antibody selection from phage display libraries -- Part IV: Engineering and production of immunoglobulins -- Aspects of isotype selection -- Generation of heavy and light chains (chimeric antibodies) -- Humanising antibodies by CDR grafting -- Humanization by resurfacing -- Human antibodies by guided selection -- In silico de-immunization -- Affinity maturation by chain shuffling and site directed mutagenesis -- Affinity maturation by random mutagenesis and phage display -- Engineering of the Fc Region for Improved PK (FcRn Interaction) -- Antibody-dependent Enzyme Prodrug Therapy (ADEPT) -- Production of recombinant human IgG antibodies in the baculovirus expression system -- Expression of IgA molecules in mammalian cells -- Part V: Antibody characterization -- Expression of complete antibodies in transgenic plants -- Expression of full length monoclonal antibodies (mAb) in algal chloroplast -- Expression of IgG Antibodies in mammalian cells -- Protein A/G chromatography -- Epitope analysis using synthetic peptide repertoires prepared by SPOT synthesis technology -- Epitope mapping by printed peptide libraries -- Antibody epitope mapping using yeast display -- Size exclusion chromatography -- Structural characterization of antibodies by mass spectrometry -- Antibody glycans characterization -- Affinity measurements by competition ELISA -- Anti-histidine antibodies as tools for reversible capturing of His-tagged fusion proteins for subsequent binding analysis -- Affinity measurements using quartz crystal microbalance (QCM) -- Affinity measurements with radiolabeled antibodies -- Neutralization tests -- Functional characterization of antibodies neutralizing soluble factors in vitro and in vivo -- Competitive ELISA -- Quantification of human IgG and related Fc fusion proteins by a human IgG/Fc capture ELISA -- Determination of Fc-mediated antibody-effector functions by chromium release assay -- Binding studies with flow cytometry -- Index."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/3944155247> ;
    schema:genre "Laboratory manuals"@en ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/477295483> ;
    schema:name "Antibody engineering. Volume 1"@en ;
    schema:productID "630107729" ;
    schema:publication <http://www.worldcat.org/title/-/oclc/630107729#PublicationEvent/berlin_london_springer_2010> ;
    schema:publisher <http://experiment.worldcat.org/entity/work/data/3944155247#Agent/springer> ; # Springer
    schema:url <http://link.springer.com/10.1007/978-3-642-01144-3> ;
    schema:url <http://www.myilibrary.com?id=283054> ;
    schema:url <http://dx.doi.org/10.1007/978-3-642-01144-3> ;
    schema:url <http://gate.lib.buffalo.edu/login?url=http://link.springer.com/book/10.1007/978-3-642-01144-3> ;
    schema:url <http://public.eblib.com/choice/publicfullrecord.aspx?p=571463> ;
    schema:url <https://link.springer.com/openurl?genre=book&isbn=978-3-642-01143-6> ;
    schema:url <https://grinnell.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-642-01144-3> ;
    schema:url <http://www.springerprotocols.com/BookToc/doi/10.1007/978-3-642-01144-3> ;
    schema:url <http://site.ebrary.com/id/10372355> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-3-642-01143-6> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-3-642-01144-3> ;
    schema:workExample <http://worldcat.org/isbn/9783642011443> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/630107729> ;
    .


Related Entities

<http://dbpedia.org/resource/London> # London
    a schema:Place ;
    schema:name "London" ;
    .

<http://experiment.worldcat.org/entity/work/data/3944155247#Topic/antibodies_genetics> # Antibodies--genetics
    a schema:Intangible ;
    schema:name "Antibodies--genetics"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/3944155247#Topic/antibody_formation_genetics> # Antibody Formation--genetics
    a schema:Intangible ;
    schema:name "Antibody Formation--genetics"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/3944155247#Topic/dna_recombinant_immunology> # DNA, Recombinant--immunology
    a schema:Intangible ;
    schema:name "DNA, Recombinant--immunology"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/3944155247#Topic/genetic_engineering_methods> # Genetic Engineering--methods
    a schema:Intangible ;
    schema:name "Genetic Engineering--methods"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/3944155247#Topic/sciences_de_la_vie> # Sciences de la vie
    a schema:Intangible ;
    schema:name "Sciences de la vie"@en ;
    .

<http://id.loc.gov/authorities/subjects/sh99001981> # Recombinant antibodies
    a schema:Intangible ;
    schema:name "Recombinant antibodies"@en ;
    .

<http://id.worldcat.org/fast/1091483> # Recombinant antibodies
    a schema:Intangible ;
    schema:name "Recombinant antibodies"@en ;
    .

<http://viaf.org/viaf/57496664> # Roland Kontermann
    a schema:Person ;
    schema:birthDate "1961" ;
    schema:familyName "Kontermann" ;
    schema:givenName "Roland" ;
    schema:name "Roland Kontermann" ;
    .

<http://viaf.org/viaf/64255356> # Stefan Dübel
    a schema:Person ;
    schema:familyName "Dübel" ;
    schema:givenName "Stefan" ;
    schema:name "Stefan Dübel" ;
    .

<http://worldcat.org/isbn/9783642011443>
    a schema:ProductModel ;
    schema:isbn "3642011446" ;
    schema:isbn "9783642011443" ;
    .

<http://www.worldcat.org/oclc/477295483>
    a schema:CreativeWork ;
    rdfs:label "Antibody engineering. Volume 1." ;
    schema:description "Print version:" ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/630107729> ; # Antibody engineering. Volume 1
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.