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Antigen processing : methods and protocols

Author: Peter van Endert
Publisher: New York : Humana Press, ©2013.
Series: Methods in molecular biology (Clifton, N.J.), 960.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In Antigen Processing: Methods and Protocols, expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Peter van Endert
ISBN: 9781627032186 1627032185
OCLC Number: 826630693
Language Note: English.
Description: 1 online resource (xvii, 589 pages) : color illustrations.
Contents: Purification of large cytosolic proteases for in vitro assays: 20S and 26S proteasomes --
Analysis of proteasome generated antigenic peptides by mass spectrometry --
Using protease inhibitors in antigen presentation assays --
Kinetic studies of cytoplasmic antigen processing and production of MHC class I ligands --
Assaying peptide translocation by the peptide transporter TAP --
In vitro reconstitution of the MHC class I peptide-loading complex --
Studying mhc class i peptide loading and exchange in vitro --
Measuring synthesis and degradation of MHC class I molecules --
Studying ubiquitination of MHC class I molecules --
Evaluation of viral interference with MHC class I-restricted antigen processing and presentation using a flow cytometry-based approach --
Determining the activity of the transporter associated with antigen processing in the compartments of the secretory pathway --
Biochemical large-scale identification of MHC class I ligands --
Establishing MHC class I peptide motifs --
Quantitating MHC class I ligand production and presentation using TCR-like antibodies --
Biochemical analysis of naturally processed antigenic peptides presented by MHC class I molecules --
Identifying source proteins for MHC class I-presented peptides --
Purification, preparation, and use of chaperone-peptide complexes for tumor immunotherapy --
Recombinant poxviruses: Versatile tools for immunological assays --
Bioinformatics identification of antigenic peptide: Predicting the specificity of major MHC class I and II pathway players --
Evaluating CD8(+) T cell responses in vitro --
Cloning CD8(+) cytolytic T lymphocytes --
Production of CD4(+) and CD8 (+) T cell hybridomas --
Tracking antigen-specific CD8(+) T cells using MHC class I multimers --
The purification of large numbers of antigen presenting dendritic cells from mouse spleen --
Preparation of dendritic cells by in vitro cultures --
Monitoring dendritic cell activation and maturation --
Monitoring the intracellular routing of internalized antigens by immunofluorescence microscopy --
Isolation of a specialized, antigen-loaded early endosomal subpopulation by flow cytometry --
Preparing antigens suitable for cross-presentation assays in vitro and in vivo --
Gene transduction in human monocyte-derived dendritic cells using lentiviral vectors --
Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading --
Assembly of matched [alpha/beta] subunits to HLA class II peptide receptors --
Studying MHC class II peptide loading and editing in vitro --
Study of the allogeneic response induced by endothelial cells expressing hla class ii after lentiviral transduction --
Antigen processing for mhc presentation via macroautophagy --
Studying MHC class II transport in dendritic cells --
In vitro digestion with proteases producing MHC class II ligands --
MHC-II ubiquitination --
Studying MHC class II presentation of immobilized antigen by B lymphocytes --
Production of primary human CD4(+) T cell lines and clones --
Analyzing antigen recognition by natural killer T cells --
Chromatofocusing purification of CD1B-antigen complexes and their analysis by isoelectric focusing.
Series Title: Methods in molecular biology (Clifton, N.J.), 960.
Responsibility: edited by Peter van Endert.

Abstract:

This Methods in Molecular Biology (TM) book offers a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II  Read more...

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