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Antiviral methods and protocols

Author: Edwin Yunhao Gong; Springer Science+Business Media,
Publisher: New York : Humana Press, [2013]
Series: Methods in molecular biology (Clifton, N.J.), v. 1030.; Springer protocols (Series)
Edition/Format:   Print book : English : Second editionView all editions and formats

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Genre/Form: Laboratory Manuals
Document Type: Book
All Authors / Contributors: Edwin Yunhao Gong; Springer Science+Business Media,
ISBN: 9781627034838 1627034838
OCLC Number: 837136020
Description: xiv, 452 pages : illustrations.
Contents: Part I Human immunodeficiency virus type 1 (HIV-1) --
Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors / Peggy Geluykens [and others] --
Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion / Liesbet Smeulders [and others] --
Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay / Bertrand Van Schoubroeck [and others] --
Biochemical screening assays to identify HIV-1 integrase inhibitors / Marleen Clynhens [and others] --
HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance / Peter Van den Eede [and others] --
Part II Hepatitis viruses --
In vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis / Rumin Zhang and William T. Windsor --
Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template / Eric Ferrari and Hsueh-Cheng Huang --
Selecting and characterizing drug-resistant hepatitis C virus replicon / Inge Vliegen, Leen Delang, and Johan Neyts --
Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors / Leen Vijgen [and others] --
Expression and purification of hepatitis C virus protease from clinical samples / Liesbet van der Helm --
Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase / Erna Cleiren, Benoit Devogelaere, and Katleen Fierens --
Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b / Diana Koletzki [and others] --
Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures / Dawei Cai [and others] --
In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates / Yang Liu and Kathryn M. Kitrinos --
Part III Flaviviruses --
Cell-based antiviral assays for screening and profiling inhibitors against dengue virus / Edwin Yunhao Gong [and others] --
Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes / Edwin Yunhao Gong [and others] --
High-throughput screening using dengue virus reporter genomes / Wolfgang Fischl and Ralf Bartenschlager --
Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate / Christoph Nitsche and Christian D. Klein --
Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase / Edwin Yunhao Gong [and others] --
Detection and quantification of flavivirus NS5 methyl- transferase activities / Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi --
Testing antiviral compounds in a dengue mouse model / Wouter Schul, Andy Yip, and Pei-Yong Shi --
Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds / Sofia L. Alcaraz-Estrada, Erin Donohue Reichert, and Radhakrishnan Padmanabhan --
Part IV Herpes viruses --
Methods for screening and profiling inhibitors of herpes simplex viruses / Edwin Yunhao Gong --
In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models / Frances Valencia, Ronald L. Veselenak, and Nigel Bourne --
Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors / Christel Van den Eynde [and others] --
Part V Respiratory viruses --
Fluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus / Leen Kwanten, Ben De Clerck, and Dirk Roymans --
Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells / Anna Lundin, Tomas Bergström, and Edward Trybala --
Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models / Joke Van den Berg, Leen Kwanten, and Dirk Roymans --
In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model / Lieselot Houspie [and others] --
Methods to determine mechanism of action of anti-influenza inhibitors / Angela Luttick, Stephanie Hamilton, and Simon P. Tucker --
Methods for evaluation of antiviral efficacy against influenza virus infections in animal models / Donald F. Smee and Dale L. Barnard --
Part VI Alphaviruses --
Development of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell / Edwin Yunhao Gong [and others] --
Mouse model of chikungunya virus with utility in antiviral studies / Ashley Dagley and Justin G. Julander.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1030.; Springer protocols (Series)
Responsibility: edited by Edwin Yunhao Gong.
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