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Antiviral methods and protocols

Author: Edwin Yunhao Gong
Publisher: New York : Humana Press, [2013] ©2013
Series: Methods in molecular biology (Clifton, N.J.), v. 1030.
Edition/Format:   eBook : Document : English : Second editionView all editions and formats
Summary:
Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations associated with the existing antiviral drugs, such as adverse effects and emergence of drug-resistant mutations.  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Antiviral methods and protocols.
[S.l.] : Humana Press Inc, 2013
(OCoLC)832313879
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Edwin Yunhao Gong
ISBN: 9781627034845 1627034846
OCLC Number: 852431492
Description: 1 online resource (xiv, 452 pages) : illustrations (some color).
Contents: Part I Human immunodeficiency virus type 1 (HIV-1) --
Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors / Peggy Geluykens, Koen Acker, Johan Vingerhoets, Christel Eynde, Marnix Loock, Géry Dams --
Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion / Liesbet Smeulders, Lieve Bunkens, Inge Vereycken, Koen Acker, Pascale Holemans, Emmanuel Gustin, Marnix Loock, Géry Dams --
Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay / Bertrand Van Schoubroeck, Marnix Loock, Tania Ivens, Pascale Dehertogh, Dirk Jochmans, Géry Dams --
Biochemical screening assays to identify HIV-1 integrase inhibitors / Marleen Clynhens, Alexandra Smets, Inge Vereycken, Marnix Loock, Reginald Clayton, Geert Meersseman, Olivia Goethals --
HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance / Peter Van den Eede, Liesbeth Wesenbeeck, Yvan Verlinden, Maxim Feyaerts, Veerle Smits, Ann Verheyen, Leen Vanhooren, Alain Deloof, Jorge Villacian, Theresa Pattery --
Part II Hepatitis viruses --
In vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis / Rumin Zhang and William T. Windsor --
Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template / Eric Ferrari and Hsueh-Cheng Huang --
Selecting and characterizing drug-resistant hepatitis C virus replicon / Inge Vliegen, Leen Delang, and Johan Neyts --
Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors / Leen Vijgen, Jannick Verbeeck, Barbara Kerckhove, Jan Martin Berke, Diana Koletzki, Gregory Fanning, Oliver Lenz --
Expression and purification of hepatitis C virus protease from clinical samples / Liesbet van der Helm --
Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase / Erna Cleiren, Benoit Devogelaere, and Katleen Fierens --
Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b / Diana Koletzki, Theresa Pattery, Bart Fevery, Leen Vanhooren, Lieven J. Stuyver --
Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures / Dawei Cai, Hui Nie, Ran Yan, Ju-Tao Guo, Timothy M. Block, Haitao Guo --
In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates / Yang Liu and Kathryn M. Kitrinos --
Part III Flaviviruses --
Cell-based antiviral assays for screening and profiling inhibitors against dengue virus / Edwin Yunhao Gong, Marleen Clynhens, Tania Ivens, Pedro Lory, Kenny Simmen, Guenter Kraus --
Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes / Edwin Yunhao Gong, Alexandra Smets, Nick Verhayen, Marleen Clynhens, Emmanuel Gustin, Pedro Lory, Guenter Kraus --
High-throughput screening using dengue virus reporter genomes / Wolfgang Fischl and Ralf Bartenschlager --
Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate / Christoph Nitsche and Christian D. Klein --
Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase / Edwin Yunhao Gong, Jean-François Bonfanti, Tania Ivens, Marijke Auwera, Barbara Kerckhove, Guenter Kraus --
Detection and quantification of flavivirus NS5 methyl- transferase activities / Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi --
Testing antiviral compounds in a dengue mouse model / Wouter Schul, Andy Yip, and Pei-Yong Shi --
Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds / Sofia L. Alcaraz-Estrada, Erin Donohue Reichert, and Radhakrishnan Padmanabhan --
Part IV Herpes viruses --
Methods for screening and profiling inhibitors of herpes simplex viruses / Edwin Yunhao Gong --
In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models / Frances Valencia, Ronald L. Veselenak, and Nigel Bourne --
Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors / Christel Van den Eynde, Ellen Damme, Tania Ivens, Edwin Yunhao Gong --
Part V Respiratory viruses --
Fluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus / Leen Kwanten, Ben De Clerck, and Dirk Roymans --
Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells / Anna Lundin, Tomas Bergström, and Edward Trybala --
Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models / Joke Van den Berg, Leen Kwanten, and Dirk Roymans --
In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model / Lieselot Houspie, Hans Stevens, Maina Ngotho, Els Keyaerts, Gabriela Ispas, René Verloes, Marc Ranst, Piet Maes --
Methods to determine mechanism of action of anti-influenza inhibitors / Angela Luttick, Stephanie Hamilton, and Simon P. Tucker --
Methods for evaluation of antiviral efficacy against influenza virus infections in animal models / Donald F. Smee and Dale L. Barnard --
Part VI Alphaviruses --
Development of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell / Edwin Yunhao Gong, Jean-François Bonfanti, Tania Ivens, Marijke Auwera, Barbara Kerckhove, Guenter Kraus --
Mouse model of chikungunya virus with utility in antiviral studies / Ashley Dagley and Justin G. Julander.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1030.
Responsibility: edited by Edwin Yunhao Gong, Janssen Infectious Diseases-Diagnostics BVBA, Johnson & Johnson Corporation, Beerse, Belgium.

Abstract:

Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations associated with the existing antiviral drugs, such as adverse effects and emergence of drug-resistant mutations. The second edition of Antiviral Methods and Protocols features: All chapters are new and written by experts in the field, reflecting the major recent technical advances in antiviral research and discovery. This edition focuses on many important human viruses, such as human immunodeficiency virus type 1 (HIV-1), hepatitis viruses (hepatitis B and C viruses), herpes viruses, human respiratory syncytial virus (RSV), and influenza virus, while also featuring some important emerging viruses, such as dengue virus, West Nile virus, and chikungunya virus. As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Antiviral Methods and Protocols, Second Edition will serve as an excellent laboratory reference for pharmaceutical and academic biologists, medicinal chemists, and pharmacologists as well as for virologists in the field of antiviral research and drug discovery.

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