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Astrocytes : methods and protocols

Author: Richard Milner, Professor
Publisher: New York : Human Press, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 814.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma. Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Astrocytes.
New York : Humana Press ; Springer, ©2012
(OCoLC)744297168
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Richard Milner, Professor
ISBN: 9781617794520 161779452X
OCLC Number: 769667324
Language Note: English.
Description: 1 online resource (xiv, 575 pages) : illustrations (some color).
Contents: Astrocytes : multitalented stars of the central nervous system / Bruce R. Ransom and Christoher B. Ransom --
Neurogenic astrocytes and their glycoconjugates : not just "glue" anymore / Dennis A. Steindler --
Heterogeneity of astrocytic form and function / Nancy Ann Oberheim, Steven A. Goldman, and Maiken Nedergaard --
Preparation of mixed glial cultures from postnatal rat brain / Jean de Vellis and Ruth Cole --
Generation of primary astrocyte cultures devoid of contaminating microglia / Tracy F. Uliasz [and others] --
Derivation of microglia-free astrocyte cultures from neural stem cells / Jennifer V. Welser and Richard Milner --
Isolation and culture of spinal cord astrocytes / Amber E. Kerstetter and Robert H. Miller --
Isolation and culture of astrocytes from the retina and optic nerve / Thomas J. Lukas and Ai Ling Wang --
Primary cultures of astrocytes from fetal bovine brain / Cristina Ballarin and Antonella Peruffo --
Isolation and cultivation of porcine astrocytes / Dennis J. Bobilya --
Isolation and culture of human astrocytes / Ariane Sharif and Vincent Prevot --
Cell culturing of Caenorhabditis elegans glial cells for the assessment of cytosolic Ca² dynamics / Randy F. Stout Jr. and Vladimir Parpura --
Astrocyte polarization and wound healing in culture : studying cell adhesion molecules / H. Peng and S. Caronetto --
An in vitro trauma model to study rodent and human astrocyte reactivity / Ina-Beate Wanner --
Use of gel zymography to examine matrix metalloproteinase (gelatinase) expression in brain tissue or in primary glial cultures / Harald Frankowski [and others] --
ELISA methodology to quantify astrocyte production of cytokines/chemokines in vitro / Hongwei Qin and Etty N. Benveniste --
Induction of nitric oxide synthase-2 expression and measurement of nitric oxide production in enriched primary cortical astrocyte cultures / James A. Hewett and Sandra J. Hewett --
Examining potassium channel function in astrocytes / Michelle Olsen --
Connexin-based channels in astrocytes : how to study their properties / Christian Giaume [and others] --
Isotope-based quantitation of uptake, release, and metabolism of glutamate and glucose in cultured astrocytes / Leif Hertz --
An in vitro model of reactive astrogliosis and its effect on neuronal growth / Panpan Yu [and others] --
A neuron-astrocyte co-culture system to investigate astrocyte-secreted factors in mouse neuronal development / Emma V. Jones, Denise Cook, and Keith K. Murai --
Cocultures of neurons and astrocytes as a model for examining hypoxia-induced neuronal death / Giuliano Rita, Terho Emily, and David A. Rempe --
Use of astrocyte-microglial cocultures to examine the regulatory influence of astrocytes on microglial activation / Jennifer V. Welser and Richard Milner --
Astrocyte-Schwann-cell coculture systems / Fardad T. Afshari and James W. Fawcett --
An in vitro assay to examine oligodendrocyte precursor cell migration on astrocytes / Fardad T. Afshari and James W. Fawcett --
Investigation of astrocyte-oligodendrocyte interactions in human cultures / Gareth R. John --
An improved in vitro blood-brain barrier model : rat brain endothelial cells co-cultured with astrocytes / N. Joan Abbott [and others] --
In vitro models of the blood-brain barrier / Kaushik K. Shah, Li Yang, and Thomas J. Abbruscato --
An in vitro model of ischemic stroke / Li Yang, Kaushik K. Shah, and Thomas J. Abbruscato --
Pericyte coculture models to study astrocyte, pericyte, and endothelial cell interactions / Vladimir Katyshev and Paula Dore-Duffy --
Reprogramming of postnatal astroglia of the mouse neocortex into functional, synapse-forming neurons / Christophe Heinrich, Magdalena Götz, and Benedikt Berninger --
Assessment of glial function in the in vivo retina / Anja I. Srienc [and others] --
Imaging in vivo astrocyte/endothelial cell interactions at the blood-brain barrier / Colin L. Willis --
Transgenic techniques for cell ablation or molecular deletion to investigate functions of astrocytes and other GFAP-expressing cell types / Michael V. Sofroniew --
In vivo imaging of Ca² signaling in astrocytes using two-photon laser scanning fluorescent microscopy / Shinghua Ding --
Use of Cre-lox technology to analyze integrin functions in astrocytes / Aaron K. Mobley and Joseph H. McCarty.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 814.
Responsibility: edited by Richard Milner.

Abstract:

This Methods in Molecular Biology (TM) book is a comprehensive guide to techniques for astrocyte cell culture, and more specialized approaches for studying astrocyte functions, both in vitro and in  Read more...

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