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Basidiospore allergens: analysis of <i>Coprinus quadrifidus</i> spore, cap, and stalk extracts
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Basidiospore allergens: analysis of Coprinus quadrifidus spore, cap, and stalk extracts

Author: W E DAVIS Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.; W E HORNER Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.; J E SALVAGGIO Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.; S B LEHRER Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.
Edition/Format: Article Article : English
Publication:Clinical & Experimental Allergy, v18 n3 (May 1988): 261-267
Other Databases: WorldCat
Summary:
Atopic individuals with symptoms of respiratory allergy have been shown to have IgE-mediated reactions to spores from the basidiomycete fungi. Because our earlier studies suggested that parts of the fungus other than spores may contain allergens, the current study was performed. Extracts of Coprinus quadrifidus spores, caps, and stalks were prepared and fractionated by gel filtration column chromatography on Sephadex G-75. Analysis of column fractions of each separation by ultraviolet absorption demonstrated at least three peaks of absorbency in spore, cap, and stalk extracts. Pooled column fractions were analysed by direct radio-allergosorbent test (RAST) using pooled sera from C. quadrifidus skin-test positive subjects. Enhanced allergenic activity was present in the same portion of the column eluate for cap, spore, and stalk fractionations, corresponding to a molecular weight of approximately 10.5-25 kD. Pools with allergenic activity were used to test volunteers by skin prick and RAST. Skin test and RAST activities were similar for each of the three Coprinus extracts, with stalk being the most potent. Evidence of common allergenic epitopes was demonstrated by inhibition of spore RAST by spore, cap, and stalk extracts. These results suggest that C. quadrifidus cap and stalk extracts contain allergens similar to those in spores extract and may provide useful sources of allergen for further study.  Read more...
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Details

Document Type: Article
All Authors / Contributors: W E DAVIS Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.; W E HORNER Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.; J E SALVAGGIO Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.; S B LEHRER Affiliation: Section of Allergy-Immunology, Department of Medicine, Tulane University Medical School, New Orleans, Louisiana, U.S.A.
ISSN:0954-7894
Language Note: English
Unique Identifier: 5155017540
Notes: Dr S. B. Lehrer, Clinical Immunology Section, Tulane Medical Center, 1700 Perdido Street, New Orleans, LA 70112, U.S.A.
Received in original form 25 August 1987 and in revised form 13 November 1987; accepted for publication 23 November 1987
Number of References: 17
Awards:

Abstract:

Atopic individuals with symptoms of respiratory allergy have been shown to have IgE-mediated reactions to spores from the basidiomycete fungi. Because our earlier studies suggested that parts of the fungus other than spores may contain allergens, the current study was performed. Extracts of Coprinus quadrifidus spores, caps, and stalks were prepared and fractionated by gel filtration column chromatography on Sephadex G-75. Analysis of column fractions of each separation by ultraviolet absorption demonstrated at least three peaks of absorbency in spore, cap, and stalk extracts. Pooled column fractions were analysed by direct radio-allergosorbent test (RAST) using pooled sera from C. quadrifidus skin-test positive subjects. Enhanced allergenic activity was present in the same portion of the column eluate for cap, spore, and stalk fractionations, corresponding to a molecular weight of approximately 10.5-25 kD. Pools with allergenic activity were used to test volunteers by skin prick and RAST. Skin test and RAST activities were similar for each of the three Coprinus extracts, with stalk being the most potent. Evidence of common allergenic epitopes was demonstrated by inhibition of spore RAST by spore, cap, and stalk extracts. These results suggest that C. quadrifidus cap and stalk extracts contain allergens similar to those in spores extract and may provide useful sources of allergen for further study.

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