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Biomedical Electron Microscopy : Illustrated Methods and Interpretations.

Author: Arvid B Maunsbach; Björn A Afzelius
Publisher: Burlington : Elsevier, 1998.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry,  Read more...
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Details

Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Arvid B Maunsbach; Björn A Afzelius
ISBN: 9780080528090 0080528090
OCLC Number: 437208893
Description: 1 online resource (569 pages)
Contents: Front Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; CHAPTER 2. FIXATIVES; CHAPTER 3. FIXATIVE VEHICLE; CHAPTER 4. FIXATIVE APPLICATION; CHAPTER 5. DEHYDRATION AND EMBEDDING; CHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING; CHAPTER 7. SUPPORT FILMS; CHAPTER 8. ULTRAMICROTOMY; CHAPTER 9. SECTION-STAINING; CHAPTER 10. MICROSCOPY; CHAPTER 11. IMAGE RECORDING; CHAPTER 12. PHOTOGRAPHIC AND DIGITAL PRINTING; CHAPTER 13. NEGATIVE STAINING; CHAPTER 14. AUTORADIOGRAPHY; CHAPTER 15. CYTOCHEMISTRY. CHAPTER 16. IMMUNOCYTOCHEMISTRYCHAPTER 17. FREEZE FRACTURING AND SHADOWING; CHAPTER 18. SAMPLING AND QUANTITATION; CHAPTER 19. IMAGE PROCESSING; CHAPTER 20. THREE-DIMENSIONAL RECONSTRUCTIONS; APPENDIX: PRACTICAL METHODS; AUTHOR BIOGRAPHIES; ACKNOWLEDGMENTS FOR REPRODUCTION OF FIGURES; INDEX.

Abstract:

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.

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