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|Additional Physical Format:||Print version:
Shaked, Natan T.
Biomedical Optical Phase Microscopy and Nanoscopy
Burlington : Elsevier Science, c2012
|Material Type:||Document, Internet resource|
|Document Type:||Internet Resource, Computer File|
|All Authors / Contributors:||
Natan T Shaked; Zeev Zalevsky; Lisa L Satterwhite
|Notes:||6.4 Refractive Index Determination of Cells in Suspension.
Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC)--microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied.
|Description:||1 online resource (428 p.)|
|Contents:||Front Cover; Biomedical Optical Phase Microscopy and Nanoscopy; Copyright Page; Contents; Preface; 1. Phase Contrast Microscopy and Differential Interference Contrast Microscopy; 1 Phase Contrast Microscopy; 1.1 Introduction; 1.2 How Phase Contrast Works; 1.2.1 Basic Overview; 1.2.2 What Do You Actually See in a Phase Contrast Image?; 1.2.3 Positive and Negative Phase; 1.2.4 Halo Artifacts; 1.3 How to Acquire Phase Contrast Images; 1.3.1 What Components Are in a Phase Contrast System?; 1.3.2 General Alignment; 1.3.3 Phase Ring Alignment; 1.3.4 Adjustability; 1.4 Experimental Uses. 1.4.1 Cell Culture Models1.4.2 Image Analysis; 1.4.3 Fluorescence Overlay; 1.5 Limitations of Phase Optics; References; Further Reading; 2 Differential Interference Microscopy; 2.1 Introduction; 2.2 Measuring Shear Angle of DIC Prism; 2.3 Bias Optimization; 2.4 A Quantitative OI-DIC Microscope with Fast Modulation of Bias and Shear Direction; 2.5 Combination of OI-DIC and Orientation-Independent Polarization Imaging; 2.6 Combination of OI-DIC and Fluorescence Imaging; Acknowledgment; References; 3 Long-Term Recordings of Live Human Cells Using Phase Contrast Microscopy; 3.1 Introduction. 3.2 The Microscope3.2.1 Illumination; 3.2.2 Temperature Control; 3.2.3 Other Considerations; 3.2.4 Observation Chambers; 3.2.5 Preparing Chambers; 3.3 Correlative Live Cell-Fixed Cell Observations; References; Further Reading; 4 Phase Imaging in Plant Cells and Tissues; 4.1 Out of Focus Imaging; 4.2 Dark Field; 4.3 Phase Contrast Techniques; 4.4 Becke Line in Optical Microscopy; 4.5 DIC Microscopy; 4.6 Hoffmann Modulation Contrast; 4.7 Adaptive Optics Microscopy; 4.8 Interferometric Microscopy; 4.9 Second Harmonic Imaging Microscopy. 4.10 Magnetic Resonance Imaging of Plants Showing the Flow ComponentAcknowledgments; References; 2. Digital Holographic Phase Microscopy; 5 Digital Holographic Microscopy for Measuring Biophysical Parameters of Living Cells; 5.1 Introduction; 5.2 Technical Introduction; 5.2.1 Classical Holography; 5.2.2 From Classical to Digital Holography; 5.2.3 Digital Holography Methods; 5.2.4 Cell Imaging and Quantitative Phase Signal Interpretation; 5.3 Biological Applications; 5.3.1 Extended Depth of Focus and 3D Tracking; 5.3.2 Dry Mass and Cell Cycle; 5.3.3 Biophysical Parameters in Hematology. 5.3.4 Cell Membrane Fluctuations5.3.5 Neuroscience; 5.3.6 Neuronal Cell Death; 5.3.7 Future Perspectives; Acknowledgments; References; 6 Holographic Microscopy Techniques for Multifocus Phase Imaging of Living Cells; 6.1 Introduction; 6.2 DHM Setups for Live Cell Imaging; 6.2.1 Fiber Optic Modular DHM; 6.2.2 Self-Interference DHM; 6.3 Recording and Numerical Evaluation of Digital Holograms; 6.3.1 Intensity Distribution in the Hologram Plane; 6.3.2 Spatial Phase Shifting-Based Reconstruction of Digital Holograms; 6.3.3 Quantitative Phase Imaging; 6.3.4 Subsequent Refocusing and Autofocusing.|