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Biomembrane protocols. Volume 27, Part 2, Architecture and function

Publisher: Totowa, N.J. : Humana Press, 1993.
Series: Methods in molecular biology (Clifton, N.J.), volume 27, pt. 2.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

There have been many important advances in our understanding of biological membrane structure and function over the last decade. Architecture and Function is concerned exclusively with the  Read more...

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Genre/Form: Fulltext
Internet Resource
Laboratory Manual
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
ISBN: 0896032507 9780896032507
OCLC Number: 245540982
Description: 1 online resource (computer files.).
Contents: Crystallization of Membrane Proteins for X-Ray Analysis. Determination of Cell Surface Polarity by Solid-Phase Lactoperoxidase Iodination. Biochemical Methods to Determine Cell Surface Topography: Part A: Labeling of Oxidized Glycoproteins with 3H-Borohydride; Part B: Identification and Separation of Integral Membrane Proteins Using Triton X-114; Part C: Surface-Selective Labeling of Polarized Proteins on Tight Epithelia Using Sulfo-N-Hydroxysuccinimido-Biotin (S-NHS-B). Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins: Part A: Preparation of Antibodies; Part B: Affinity-Purification of Antipeptide Antibodies; Part C: Purification of Membrane Proteins by Immunoaffinity Chromatography; Part D: Competitive ELISA for Determining Membrane Protein Topology. The Production of Monoclonal Antibodies to Membrane Proteins: Part A: Production of Hybridoma Cell Lines; Part B: Screening for Monoclonal Antibody Production. Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles. Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy. Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy. Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes. Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools. Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry. Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking: Part A: Synthesis of Fluorescent Glycerolipid Probes; Part B: Delivery of Fluorescent Glycerophospholipid Probes to Cultured Cells. Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids. Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes Extraction and Assay of Cyclic Nucleotides. Analysis of G-Proteins Regulating Signal Transduction Pathways: Part A: Identification of G-Proteins; Part B: GTPase Studies; Part C: Reconstitution of Second Messenger Pathways in Permeabilized Cell Preparations Using GTP Analogs and Receptor Ligands. Assay of Protein Kinases and Protein Phosphorylation: Part A: Protein Kinase-Mediated Phosphorylation Events; Part B: Measurement of Protein Kinase Expression; Part C: Measurement of Protein Kinase Activity and Translocation. Analysis of Cellular Phosphoinositides and Phosphoinositols: Extraction and Simple Analytical Procedures: Part A: Biosynthesis and Extraction of Phosphoinositides and Phosphoinositols; Part B: Separation of Phosphoinositols by Anion-Exchange Chromatography; Part C: Separation of Phosphoinositides by Thin-Layer Chromatography; Part D: Deacylation of Phospholipids and Separation of Products by Anion-Exchange Chromatography. Analysis of Cellular Phosphoinositides and Phosphoinositols by High Performance Liquid Chromatography: Part A: Sample and Standards Preparation; Part B: HPLC Analytical Methods. Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes. Membrane Permeabilization with Bacterial Toxins. Measurement of Ion Fluxes and pH Gradients Across Cell Membranes. Ligand Binding and Processing: The Perfused Liver as a Model System. The Binding of Protein-Ligands to Cell-Surface Receptors. Appendix: 1. Density Gradient Media. Appendix: 2. Balanced Salt Solutions. Index.
Series Title: Methods in molecular biology (Clifton, N.J.), volume 27, pt. 2.
Responsibility: edited by John M. Graham, Joan A. Higgins.

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