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Biomembrane protocols

Author: J M Graham; Joan A Higgins
Publisher: Totowa, N.J. : Humana Press, ©1993-1994.
Series: Methods in molecular biology (Clifton, N.J.), v. 19, etc.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

The practitioners in this book combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise  Read more...

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Additional Physical Format: Online version:
Biomembrane protocols.
Totowa, N.J. : Humana Press, ©1993-1994
(OCoLC)607800035
Online version:
Biomembrane protocols.
Totowa, N.J. : Humana Press, ©1993-1994
(OCoLC)624328409
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: J M Graham; Joan A Higgins
ISBN: 0896032361 9780896032361 0896032507 9780896032507
OCLC Number: 28024134
Description: 2 volumes : illustrations ; 23 cm.
Contents: v. 1. Isolation and analysis --
v. 2. Architecture and function. [v. 1.] The identification of subcellular fractions from mammalian cells / John M. Graham --
The isolation of nuclei and nuclear membranes from rat liver / John M. Graham --
Isolation of mitochondria, mitochondrial membranes, lysosomes, peroxisomes, and golgi membranes from rat liver / John M. Graham --
Continuous-flow electrophoresis : application to the isolation of lysosomes and endosomes / John M. Graham --
Fractionation of a microsomal fraction from rat liver or cultured cells / John M. Graham --
Isolation of plasma membrane sheets and plasma membrane domains from rat liver / Laura Scott, Michael J. Schell, Ann L. Hubbard --
The analysis of receptor-mediated endocytosis by centrifugation in isoosmotic gradients / Terry C. Ford --
The isolation of clathrin-coated vesicles and purification of their protein components / Antony P. Jackson --
Isolation of membranes from tissue culture cells / John M. Graham --
The isolation of membranes from bacteria / Robert K. Poole --
Isolation and purification of functionally intact chloroplasts from leaf tissue and leaf tissue protoplasts / David G. Whitehouse, Anthony L. Moore --
Isolation and purification of functionally intact mitochondria from plant cells / Anthony L. Moore, Anne-Catherine Fricaud, Andrew J. Walters, David G. Whitehouse --
Isolation of cholinergic-specific synaptosomes by immunoadsorption / J. Paul Luzio, Peter J. Richardson --
Separation and analysis of phospholipids by thin layer chromatography / Ian J. Cartwright --
Analysis of phospholipids by high performance liquid chromatography / Hafez A. Ahmed --
Measurement of cholesterol in membranes / Hafez A. Ahmed --
Analysis of fatty acids by gas liquid chromatography / Ian J. Cartwright --
Chemical assays for proteins / David J. Winterbourne --
Separation and analysis of membrane proteins by SDS-polyacrylamide gel electrophoresis / Michael J. Dunn, Samantha J. Bradd --
Analysis of membrane proteins by Western blotting and enhanced chemiluminescence / Samantha J. Bradd, Michael J. Dunn --
Two-dimensional polyacrylamide gel electrophoresis of membrane proteins / Joe Corbett, Michael J. Dunn --
Isolation of a membrane-bound enzyme, 5'-nucleotidase / J. Paul Luzio, Elaine M. Bailyes --
The isolation of membrane proteoglycans / Malcolm Lyon --
Oligosaccharide mapping and sequence analysis of glycosaminoglycans / Jeremy E. Turnbull --
Analysis of sugar sequences in glycoproteins by glycosidase digestion and gel filtration / Anthony P. Corfield --
The extraction and analysis of glycosphingolipids / Norman A. Gregson. [v. 2.] Crystallization of membrane proteins for X-ray analysis / Brian J. Sutton, Maninder K. Sohi --
Determination of cell-surface polarity by solid-phase lactoperoxidase lodination / William A. Muller --
Biochemical methods to determine cell-surface topography / William A. Muller --
Use of antipeptide antibodies for the isolation and study of membrane proteins / Stephen A. Baldwin --
The production of monoclonal antibodies to membrane proteins / Lynda J. Partridge --
Puriffication of a membrane protein (Ca²/Mg²-ATPase) and its reconstitution into lipid vesicles / J. Malcolm East --
Measurement of protein-protein interactions in reconstituted membrane vesicles using fluorescence spectroscopy / Anthony G. Lee --
Measurement of lipid-protein interactions in reconstituted membrane vesicles using fluorescence spectroscopy / Anthony G. Lee --
Determination of the transverse topography of membrane lipids using enzymes and covalent labels as probes / Joan A. Higgins --
Determination of the transverse topography of membrane phospholipids using phospholipid transfer proteins as tools / Joan A Higgins --
Prothrombinase complex as a tool to assess changes in membrane phospholipid asymmetry / Paul Comfurius, Edouard Bevers, Robert F. Zwaal --
Fluorescent glycerolipid probes : synthesis and use for examining intracellular lipid trafficking / Richard G. Sleight --
Synthesis and use of spin-labeled lipids for studies of the transmembrane movement of phospholipids / Pierre Fellmann, Alain Zachowski, Philippe F. Devaux --
Measurement of membrane fluidity and membrane fusion with fluorescent probes / Lindsay Bashford C. --
Extraction and assay of cyclic nucleotides / Guy St. Whitley --
Analysis of G-proteins regulating signal transduction pathways / Margaret M. Harnett --
Assay of protein kinases and protein phosphorylation / Margaret M. Harnett --
Analysis of cellular phosphoinositides and phosphoinositols by extraction and simple analytical procedures / Ian M. Bird --
Analysis of cellular phosphoinositides and phosphoinositols by high-performance liquid chromatography / Ian M. Bird --
Cytosolic free calcium measurements in single cells using calcium-sensitive fluorochromes / Mone Zaidi, A.S. Towhidul Alam, Christopher Box, Vijay Shankar, Peter J. Bevis, Christopher L. Huang, Michael Pazianas, Baljit S. Moonga --
Membrane permeabilization with bacterial toxins / C. Lindsay Bashford --
Measurement of ion fluxes and pH gradients across cell membranes / C. Lindsay Bashford --
Ligand binding and processing : the perfused liver / Carol A. Renfrew, Livia A. Casciola-Rosen, Ann L. Hubbard --
The binding of protein-ligands to cell-surface receptors / David A. Grant.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 19, etc.
Responsibility: edited by John M. Graham, Joan A. Higgins.
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