skip to content
Biomolecular kinetics : a step-by-step guide Preview this item
ClosePreview this item
Checking...

Biomolecular kinetics : a step-by-step guide

Author: Clive R Bagshaw
Publisher: Boca Raton, FL : CRC Press, 2017.
Series: Foundations of biochemistry and biophysics.
Edition/Format:   eBook : Document : English : First editionView all editions and formats
Summary:
""A gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide"-Mark Leake, University of York"destined to become a standard reference.... Not just a 'how to' handbook but also an accessible primer in the essentials of kinetic theory and practice."-Michael Geeves, University of Kent"covers the entire spectrum of approaches, from the traditional steady state methods to a thorough  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Clive R Bagshaw
ISBN: 9781315120355 1315120356 9781498727242 1498727247
OCLC Number: 993950655
Description: 1 online resource (xxiv, 454 pages)
Contents: INTRODUCTIONWhat can we learn from kinetics?Kinetic measurements: the transient- and the steady-state.Kinetic measurements: ensemble and single-molecule approaches.KINETIC THEORYOne-step irreversible reactionOne-step reversible reactionIrreversible binding reactionReversible binding reactionTwo-step irreversible reactionTwo-step reversible reactionTwo-step reversible binding reaction: induced fitTwo-step reversible binding reaction: conformational selectionThermodynamic boxesAlternative binding modeCompetitive bindingDisplacement reactionsMulti-site ligand bindingMulti-step reversible reactionsSOME UNDERLYING PHYSICAL PRINCIPLESBasic thermodynamicsThe significance of Free Energy, D GThermal energyReaction kinetics and the transition stateTime scales of binding kinetics: diffusion-controlled reactionsThe Time Scales of Macromolecular Conformational ChangesForce dependence of rate constantsThe basis of enzyme catalysisENZYME KINETICS Enzyme catalysis with a single substrateEnzyme catalysis with two substratesIdentification of the rate-limiting stepThe use of isotopesEnzyme activators and inhibitorsAllosteric and Cooperative reactions BEYOND ENZYME KINETICSProtein foldingPolymerization reactionsNucleic acid dynamicsMotor proteinsMembrane Transporters, pumps and channels Biological ClocksKinetics in vivoEXPERIMENTAL STRATEGIESSample preparationPreliminary characterizationCharacterization of equilibrium binding constantsSteady-state enzyme kineticsTransient kinetic assaysTuning the system for measurementThe use of indicatorsSingle-molecule assaysINSTRUMENTATIONThe basic componentsMixing techniquesPerturbation techniquesFlash photolysisComponents for optical detectionDetection methodsDATA ANALYSISBasic statistical conceptsAnalysis of linear functionsLeast-squares fitting to analytical expressionsGlobal fitting to analytical equationsFitting data directly to kinetic models Multi-wavelength analysisSINGLE-MOLECULE KINETICSIntroductionInstrumentation Single-molecule force measurementsAnalysis of single-molecule kinetic dataAnalysis of complex time trajectoriesAutocorrelation analysisSingle-molecule enzymologyKINETIC THINKING: BACK TO THE FUTUREBiochemical time scalesDNA binding proteinsInduced Fit versus Conformational Selection revisitedAppendicesSome useful physical constantsKinetic instrument suppliersKinetic softwareUse of spreadsheets in kinetic analysis
Series Title: Foundations of biochemistry and biophysics.
Responsibility: Clive R. Bagshaw.

Abstract:

""A gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide"-Mark Leake, University of York"destined to become a standard reference.... Not just a 'how to' handbook but also an accessible primer in the essentials of kinetic theory and practice."-Michael Geeves, University of Kent"covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques" -Stephen Halford, University of BristolThis illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A."--Provided by publisher.

Reviews

Editorial reviews

Publisher Synopsis

"a unique and innovative contribution to the field... the first to my knowledge that covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of Read more...

 
User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/993950655> # Biomolecular kinetics : a step-by-step guide
    a schema:CreativeWork, schema:Book, schema:MediaObject ;
    library:oclcnum "993950655" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/flu> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/enzymes> ; # Enzymes
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/kinetics> ; # Kinetics
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/science_life_sciences_biology> ; # SCIENCE / Life Sciences / Biology
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/anatomy> ; # Anatomy
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/physics> ; # Physics
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/science_physics> ; # SCIENCE / Physics
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/biochemical_phenomena> ; # Biochemical Phenomena
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/biochemistry> ; # Biochemistry
    schema:about <http://experiment.worldcat.org/entity/work/data/4065293368#Topic/science_chemistry_physical_&_theoretical> ; # SCIENCE / Chemistry / Physical & Theoretical
    schema:about <http://dewey.info/class/572.44/e23/> ;
    schema:author <http://experiment.worldcat.org/entity/work/data/4065293368#Person/bagshaw_clive_r> ; # Clive R. Bagshaw
    schema:bookEdition "First edition." ;
    schema:bookFormat schema:EBook ;
    schema:datePublished "2017" ;
    schema:description """A gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide"-Mark Leake, University of York"destined to become a standard reference.... Not just a 'how to' handbook but also an accessible primer in the essentials of kinetic theory and practice."-Michael Geeves, University of Kent"covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques" -Stephen Halford, University of BristolThis illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A."--Provided by publisher."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/4065293368> ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://experiment.worldcat.org/entity/work/data/4065293368#Series/foundations_of_biochemistry_and_biophysics> ; # Foundations of biochemistry and biophysics.
    schema:name "Biomolecular kinetics : a step-by-step guide"@en ;
    schema:productID "993950655" ;
    schema:url <http://public.eblib.com/choice/PublicFullRecord.aspx?p=5089127> ;
    schema:url <http://marc.crcnetbase.com/isbn/9781498727242> ;
    schema:url <http://lib.myilibrary.com?id=1040714> ;
    schema:url <https://0-www-taylorfrancis-com.pugwash.lib.warwick.ac.uk/books/9781498727242> ;
    schema:url <https://www.taylorfrancis.com/books/9781498727242> ;
    schema:url <https://stanford.idm.oclc.org/login?url=http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=1610879> ;
    schema:url <http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=1610879> ;
    schema:workExample <http://worldcat.org/isbn/9781498727242> ;
    schema:workExample <http://worldcat.org/isbn/9781315120355> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/993950655> ;
    .


Related Entities

<http://experiment.worldcat.org/entity/work/data/4065293368#Person/bagshaw_clive_r> # Clive R. Bagshaw
    a schema:Person ;
    schema:familyName "Bagshaw" ;
    schema:givenName "Clive R." ;
    schema:name "Clive R. Bagshaw" ;
    .

<http://experiment.worldcat.org/entity/work/data/4065293368#Series/foundations_of_biochemistry_and_biophysics> # Foundations of biochemistry and biophysics.
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/993950655> ; # Biomolecular kinetics : a step-by-step guide
    schema:name "Foundations of biochemistry and biophysics." ;
    schema:name "Foundations of biochemistry and biophysics" ;
    .

<http://experiment.worldcat.org/entity/work/data/4065293368#Topic/biochemical_phenomena> # Biochemical Phenomena
    a schema:Intangible ;
    schema:name "Biochemical Phenomena"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/4065293368#Topic/science_chemistry_physical_&_theoretical> # SCIENCE / Chemistry / Physical & Theoretical
    a schema:Intangible ;
    schema:name "SCIENCE / Chemistry / Physical & Theoretical"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/4065293368#Topic/science_life_sciences_biology> # SCIENCE / Life Sciences / Biology
    a schema:Intangible ;
    schema:name "SCIENCE / Life Sciences / Biology"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/4065293368#Topic/science_physics> # SCIENCE / Physics
    a schema:Intangible ;
    schema:name "SCIENCE / Physics"@en ;
    .

<http://lib.myilibrary.com?id=1040714>
    rdfs:comment "Connect to MyiLibrary resource." ;
    .

<http://worldcat.org/isbn/9781315120355>
    a schema:ProductModel ;
    schema:isbn "1315120356" ;
    schema:isbn "9781315120355" ;
    .

<http://worldcat.org/isbn/9781498727242>
    a schema:ProductModel ;
    schema:isbn "1498727247" ;
    schema:isbn "9781498727242" ;
    .

<https://0-www-taylorfrancis-com.pugwash.lib.warwick.ac.uk/books/9781498727242>
    rdfs:comment "Connect to Taylor and Francis e-book" ;
    .

<https://www.taylorfrancis.com/books/9781498727242>
    rdfs:comment "Distributed by publisher. Purchase or institutional license may be required for access." ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.