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Biophysical, chemical, and functional probes of RNA structure, interactions and folding. Part B

Author: Daniel Herschlag
Publisher: Amsterdam [Netherlands] ; Boston [Mass.] : Elsevier/Academic Press, ©2009.
Series: Methods in enzymology, v. 469.
Edition/Format:   eBook : Document : English : 1st edView all editions and formats
Summary:

Offers laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry  Read more...

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Genre/Form: Electronic books
Additional Physical Format: Print version:
Biophysical, chemical, and functional probes of RNA structure, interactions and folding. Part B.
Amsterdam [Netherlands] ; Boston [Mass.] : Elsevier/Academic Press, ©2009
(OCoLC)441761608
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Daniel Herschlag
ISBN: 0123809223 9780123809223 9780123809230 0123809231 1282699970 9781282699977
OCLC Number: 703255525
Description: 1 online resource (xlvi, 503 pages, [16] pages of plates) : illustrations (some color).
Contents: A. Chemical and Enzymatic Footprinting of RNA Structure 1. Equilibrium hydroxyl radical footprinting 2. Bench-top time-resolved hydroxyl radical footprinting 3. Analysis of hydroxyl radical footprinting gels `SAFA' 4. Kinetic modeling of reaction pathways from hydroxyl radical data 5. "BABE" mapping of protein/RNA position 6. Multiplexed....(MOHCA) 7. SHAPE 8. "In-line Probing" 9. NAIM 10. Other NAIM 11. Purification of T7 RNA Polymerase 12. Purification of T4 RNA ligase 13. In vitro transcription of RNA 14. 32P-labeling of RNA B. BIOPHYSICAL TECHNIQUES 15. Fluorescent labeling of RNAs 16. Assembly of complex RNAs by `Moore-Sharp' ligations 17. Assembly of complex RNAs by `Moore-Sharp' ligations 18. General considerations for smFRET with RNA samples 19. Ion counting 20. ASAXS 21. NLPB 22. smNLPB 23. Gel mobility mapping of junction structure 24. Temperature gradient gels 25. Melting studies 26. Co-transcriptional folding studies 27. Activity assays to follow folding processes 28. 2AP fluorescence 29. EPR measurements of RNA dynamics 30. EPR measurements of RNA dynamics 31. FPA measurements of RNA dynamics 32. EPR methods to study specific metal ion binding sites in RNA 33. Thermodynamic study of site-specific metal ion binding sites in RNA 34. Oligonucleotide hybridization studies of RNA folding pathways 35. Native gel shifts 36. Tiling arrays to assess RNA structure 37. EPR distance measurements in RNA 38. RNA folding in vivo 39. Cleavage of RNAs with `restriction DNAzymes'
Series Title: Methods in enzymology, v. 469.
Responsibility: edited by Daniel Herschlag.

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