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Bone research protocols

Author: Miep H Helfrich; Stuart Ralston
Publisher: Totowa, N.J. : Humana Press, ©2003.
Series: Methods in molecular medicine, 80.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Laboratory techniques presented here will help researchers investigating the mechanisms by which certain genes affect bone mass and strength. These reproducible methods cover such topics as the  Read more...

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Genre/Form: Laboratory manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Miep H Helfrich; Stuart Ralston
ISBN: 1588290441 9781588290441 1592593666 9781592593668
OCLC Number: 51271023
Description: xiv, 448 pages : illustrations ; 23 cm.
Contents: Human osteoblast culture / J.A. Gallagher --
Osteoblast isolation from murine calvariae and long bones / A. Bakker and J. Klein-Nulend --
Mineralizing fibroblast-colony-forming assays / A. Scutt [and others] --
Osteocyte isolation and culture / P.J. Nijweide [and others] --
Isolated osteoclast cultures / A. Hoebertz and T.R. Arnett --
Primary isolation and culture of chicken osteoclasts / P. Collin-Osdoby, F. Anderson and P. Osdoby --
Isolation and purification of rabbit osteoclasts / F.P. Coxon [and others] --
Generating human osteoclasts from peripheral blood / A. Sabokbar and N.S. Athanasou --
Generating human osteoclasts in vitro from bone marrow and peripheral blood / A.M. Flanagan and H.M. Massey --
Generating murine osteoclasts from bone marrow / N. Takahashi [and others] --
Osteoclast formation in the mouse coculture assay / R.J. van 't Hof --
RANKL-mediated osteoclast formation from murine RAW 264.7 cells / P. Collin-Osdoby [and others] --
Analysis of osteoclast function in mouse calvarial cultures / M.J. Marshall and M.N. Rowlands --
Assessing bone formation using mouse calvarial organ cultures / I.R. Garrett --
In situ hybridization and in situ reverse transcription polymerase chain reaction in human bone sections / A.P. Mee and J.A. Hoyland --
Techniques for the study of apoptosis in bone / B.S. Noble and H.Y. Stevens --
Protein localization in wax-embedded and frozen sections of bone using immunohistochemistry / S. Bord --
Detection of noncollagenous bone proteins in methylmethacrylate-embedded human bone sections / J.P. van Leeuwen and P. Derkx --
Fluorescence imaging of bone-resorbing osteoclasts by confocal microscopy / S.A. Nesbitt and M.A. Horton --
Bone histomorphometry / S. Vedi and J. Compston --
Transmission electron microscopy of bone / V. Everts, A. Niehof and W. Beertsen --
Scanning electron microscopy of bone / D. Marshall, M.H. Helfrich and R.M. Aspden --
Bone measurements by peripheral quantitative computed tomography in rodents / J.A. Gasser --
Studies of local bone remodeling. The calvarial injection assay / R.J. van 't Hof, C.E. Clarkin and K.J. Armour --
Inflammation-induced osteoporosis. The IMO model / K.J. Armour and K.E. Armour --
Ovariectomy and estrogen replacement in rodents / J.W. Chow --
Mechanical testing of bone ex vivo / R.M. Aspden --
Bone cell responses to fluid flow / H.Y. Stevens and J.A. Frangos --
Methods for analyzing bone cell responses to mechanical loading using in vitro monolayer and organ culture models / A.A. Pitsillides [and others] --
Extraction of nucleic acids from bone / T.L. Stewart and V. Mann --
Analysis of gene expression in bone by quantitative RT-PCR / D. Ireland.
Series Title: Methods in molecular medicine, 80.
Responsibility: edited by Miep H. Helfrich, Stuart H. Ralston.
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"Bone Research Protocols is a most welcome compilation of methods. In it, many of the most productive laboratories in some of the most challenging areas of cell biology reveal detailed protocols that Read more...

 
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