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Calcium signaling protocols

Author: David G Lambert
Publisher: Totowa, N.J. : Humana Press, ©2006.
Series: Methods in molecular biology (Clifton, N.J.), v. 312.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Database:WorldCat
Summary:

In the first edition of Calcium Signaling Protocols I began by writing "The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the  Read more...

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Genre/Form: Aufsatzsammlung
Laboratory manuals
Manuels de laboratoire
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: David G Lambert
ISBN: 1588294420 9781588294425 1592599494 9781592599493
OCLC Number: 57965822
Description: x, 359 pages : illustrations ; 24 cm.
Contents: Fluorescent measurement of [Ca2+]c: basic practical considerations / Alec W.M. Simpson --
Measurement of [Ca2+]i in whole cell suspensions using fura-2 / Robert A. Hirst [and others] --
Measurement of [Ca2+]i in cell suspensions using indo-1 / Adriaan Nelemans --
Confocal microscopy: theory and applications for cellular signaling / Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars --
Single cell and subcellular measurement of intracellular Ca2+ concentration / Anthony J. Morgan and Andrew P. Thomas --
Ratiometric Ca2+ measurements using the FlexStation scanning fluorometer / Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty --
Measuring Ca2+ changes in multiwell format using the fluorometric imaging plate reader / Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty. Measurement of Ca2+ entry into cells using 45Ca2+ / Mercedes Villarroya [and others] --
Measurement of [3H]PN200-110 and [125I]--conotoxin MVIIA binding / Kazuyoshi Hirota and David G. Lambert --
Whole-cell patch clamp recording of voltage-sensitive Ca2+ channel currents: heterologous expression systems and dissociated brain neurons / Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani --
Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol-labeling protocols in permeabilized cells / Alison Skippen, Philip Swigart, and Shamshad Cockcroft --
Measurement of inositol(1,4,5)triphosphate using a stereospecific radioreceptor mass assay / Darren Smart. Measurement of calcium fluxes in permeabilized cells using a 45Ca2+ uptake and release assay / Robert A. Wilcox --
Microinjection of myo-inositol(1,4,5)trisphosphate and other calcium-mobilizing agents into intact adherent cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann --
Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators / Aldebaran M. Hofer --
Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry / Hideo Kanaide --
Measurement of calcium and movement in heart cells / Leong L. Ng and Paulene A. Quinn. Simultaneous analysis of intracellular pH and Ca2+ from cell populations / Raul Martinez-Zaguilan [and others] --
Measurement of cytosolic-free Ca2+ in plant tissue / Martin R. McAinsh and Carl K.-Y. Ng --
Assay and purification of calmodulin-dependent protein kinase / Rajendra K. Sharma --
Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation / Rajendra K. Sharma --
Measurement of Ca2+-ATPase activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 312.
Responsibility: edited by David G. Lambert.
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From Reviews of the First Edition..."...will find a place on the bench in any lab that is moving into calcium signaling, or expanding its repertoire of techniques in calcium signaling. It will be Read more...

 
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