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Cancer cell culture : methods and protocols

Author: Ian A Cree
Publisher: New York : Humana Press, ©2011.
Series: Methods in molecular biology (Clifton, N.J.), v. 731.; Springer protocols (Series)
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

This new edition covers the recent advances in cancer cell culture research, including novel cell culture procedures. It includes methods of authenticating cell lines, functional assays to evaluate  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Additional Physical Format: Online version:
Cancer cell culture.
New York, N.Y. : Humana Press, ©2011
(OCoLC)721937924
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Ian A Cree
ISBN: 1617790796 9781617790799 9781617790805 161779080X
OCLC Number: 703374718
Description: xiv, 502 pages : illustrations (some color) ; 27 cm.
Contents: Xenotransplantation of breast cancers --
Feeder layers: Co-culture with nonneoplastic cells --
Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay --
Transfection and DNA-mediated gene transfer --
Analysis of drug interactions --
Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy --
Proteomic evaluation of cancer cells: Identification of cell surface proteins --
Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction --
Expression analysis of homeobox genes in leukemia/lymphoma cell lines --
Flow cytometric DNA analysis of human cancers and cell lines --
Angiogenesis assays --
Cell migration and invasion assays --
Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR --
Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay --
Purification of annexin V and its use in the detection of apoptotic cells --
Real-time cytotoxicity assays --
Differential staining cytotoxicity assay: A review --
Cell sensitivity assays: The ATP-based tumor chemosensitivity assay --
Cell sensitivity assays: The MTT assay --
Cellular chemosensitivity assays: An overview --
Isolation of endothelial cells from human tumors --
Isolation of inflammatory cells from human tumours --
Establishment and culture of leukemia-lymphoma cell lines --
Isolation and culture of ovarian cancer cells and cell lines --
Isolation and culture of squamous cell carcinoma lines --
Isolation and culture of melanoma and naevus cells and cell lines --
Isolation and culture of colon cancer cells and cell lines --
Generation of lung cancer cell line variants by drug selection or cloning --
Quality assurance and good laboratory practice --
Elimination of mycoplasmas from infected cell lines using antibiotics --
Detecting mycoplasma contamination in cell cultures by polymerase chain reaction --
Cell culture contamination --
Cytogenetic analysis of cancer cell lines --
Online verification of human cell line identity by STRDNA typing --
Characterization and authentication of cancer cell lines: An overview --
Storage of cell lines --
Principles of cancer cell culture --
Cancer biology.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 731.; Springer protocols (Series)
Responsibility: edited by Ian A. Cree.

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