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Cancer cytogenetics : methods and protocols

Author: Thomas S K Wan
Publisher: New York, N.Y. : Humana Press : Springer, ©2017.
Series: Methods in molecular biology (Clifton, N.J.), v. 1541.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting.  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Cancer cytogenetics.
New York, N.Y. : Humana Press : Springer, ©2017
(OCoLC)957533687
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Thomas S K Wan
ISBN: 9781493967032 1493967037
OCLC Number: 964971993
Description: 1 online resource (xi, 340 pages) : illustrations (some color).
Contents: Cancer cytogenetics : an introduction / Thomas S.K. Wan --
Chromosome preparation for myeloid malignancies / Eleanor K.C. Hui, Thomas S.K. Wan, and Margaret H.L. Ng --
Chromosome preparation for acute lymphoblastic leukemia / Mary Shago --
Chromosome preparation for chronic lymphoid malignancies / Dorota Koczkodaj and Agata A. Filip --
Cytogenetic harvesting of cancer cells and cell lines / Roderick A.F. MacLeod, Maren E. Kaufmann, and Hans G. Drexler --
Chromosome bandings / Huifang Huang and Jiadi Chen --
Chromosome recognition / Thomas S.K. Wan, Eleanor K.C. Hui, and Margaret H.L. Ng --
Applications of fluorescence in situ hybridization technology in malignancies / Montakarn Tansatit --
Fluorescence in situ hybridization probe preparation / Doron Tolomeo, Roscoe R. Stanyon, and Mariano Rocchi --
Fluorescence in situ hybridization probe validation for clinical use / Jun Gu, Janice L. Smith, and Patricia K. Dowling --
Fluorescence in situ hybridization on tissue sections / Alvin S.T. Lim and Tse Hui Lim --
Cytoplasmic immunoglobulin light chain revelation and interphase fluorescence in situ hybridization in myeloma / Sarah Moore, Jeffrey M. Suttle, and Mario Nicola --
Quantitative fluorescence in situ hybridization (QFISH) / Ivan Y. Iourov --
High resolution fiber-fluorescence in situ hybridization / Christine J. Ye and Henry H. Heng --
Array-based comparative genomic hybridization (aCGH) / Chengsheng Zhang, Eliza Cerveira, Mallory Romanovitch, and Qihui Zhu --
Multicolor karyotyping and fluorescence in situ hybridization-banding (MCB/mBAND) / Thomas Liehr, Moneeb A.K. Othman, and Katharina Rittscher --
Cytogenetics for biological dosimetry / Michelle Ricoul, Tamizh Gnana-Sekaran, Laure Piqueret-Stephan, and Laure Sabatier --
Recurrent cytogenetic abnormalities in myelodysplastic syndromes / Meaghan Wall --
Recurrent cytogenetic abnormalities in acute myeloid leukemia / John J. Yang, Tae Sung Park, and Thomas S.K. Wan --
Recurrent cytogenetic abnormalities in myeloproliferative neoplasms and chronic myeloid leukemia / John Swansbury --
Recurrent cytogenetic abnormalities in acute lymphoblastic leukemia / Mary Shago --
Recurrent cytogenetic abnormalities in non-hodgkin's lymphoma and chronic lymphocytic leukemia / Edmond S.K. Ma --
Recurrent cytogenetic abnormalities in multiple myeloma / Nelson Chun Ngai Chan and Natalie Pui Ha Chan --
Cytogenetic nomenclature and reporting / Marian Stevens-Kroef, Annet Simons, Katrina Rack, and Rosalind J. Hastings --
Cytogenetic resources and information / Etienne De Braekeleer, Jean-Loup Huret, Hossain Mossafa, and Philippe Dessen.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1541.
Responsibility: edited by Thomas S.K. Wan.

Abstract:

This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cancer Cytogenetics: Methods and Protocols is a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it’s also great for those who are already engaged in the field and are looking for some technical hints.

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