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Cell biology : a laboratory handbook.

Author: J E Celis
Publisher: Burlington, MA : Academic, 2005.
Series: Cell biology.
Edition/Format:   eBook : Document : English : 3rd edView all editions and formats
Summary:
This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: J E Celis
ISBN: 9780080454245 0080454240
OCLC Number: 843112769
Notes: Previous edition: San Diego, Calif.; London: Academic, 1997.
Contents of Volume 1: Part A. Cell and Tissue Culture: Associated Techniques. Section 1. General Techniques. Section 2. Culture of Specific Cell Types: Stem Cells. Section 3. Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial. Section 4. Differentiation and Reprogramming of Somatic Cells. Section 5. Immortalisation. Section 6. Somatic Cell Hybrids. Section 7. Cell Separation Techniques. Section 8. Cell Cycle Analysis. Section 9. Cytotoxic and Cell Growth Assays. Section 10. Apoptosis. Section 11. Assays of Cell Transformation, Tumorigenesis, Invasion and Wound Healing. Section 12. Electrophysiological Methods. Section 13. Organ Cultures. Part B. Viruses. Section 14. Growth and Purification of Viruses. Part C. Antibodies. Section 15. Production and Purification of Antibodies. Part D. Immunocytochemistry. Section 16. Immunofluoresence. Part E. Appendix. Contents of Volume 2: Part A. Organelles and Cellular Structures. Section 1. Isolation: Plasma Membrane, Organelles, and Cellular Structures. Section 2. Vital Staining of Cells/Organelles. Section 3. Protein Purification. Part B. Assays. Section 4. Endocytic and Exocytic Pathways. Section 5. Membranes. Section 6. Mitochondria. Section 7. Nuclear Transport. Section 8. Chromatin Assembly. Section 9. Signal Transduction Assays. Section 10. Assays and Models of in Vitro and in Vitro Motility. Section 11. Mechanical Stress in Single Cells. Part C. Appendix. Contents of Volume 3. Part A. Imaging Techniques. Section 1. Light Microscopy. Section 2. Digital Video Microscopy. Section 3. Confocal Microscopy of Living Cells and Fixed Cells. Section 4. Fluorescent Microscopy of Living Cells. Section 5. Use of Fluorescent Dyes for Studies of Intracellular Physiological Parameters. Section 6. Digital Image Processing, Analysis, Storage, and Display. Part B. Electron Microscopy. Section 7. Specimen Preparation Techniques. Section 8. Cryotechniques. Section 9. Electron Microscopy Studies of the Cytoskeleton. Section 10. Immunoelectron Microscopy. Part C. Scanning Probe and Scanning Electron Microscopy. Section 11. Scanning Probe and Scanning Electron Microscopy. Part D. Microdissection. Section 12. Tissue and Chromosome Microdissection. Part E. Tissue Arrays. Section 13. Tissue Arrays. Part F. Cytogenetics and in Situ Hybridisation Section 14. Cytogenetics. Section 15. In Situ Hybridisation. Part G. Genomics. Section 16. Genomics. Part H. Transgenic, Knockouts, and Knockdown Methods. Section 17. Transgenic, Knockouts and knock-down methods. Contents of Volume 4. Part A. Transfer of Macromolecules. Section 1. Proteins. Section 2. Genes. Section 3. Somatic Cell Nuclear Transfer. Part B. Expression Systems. Section 4. Expression Systems. Part C. Gene Expression Profiling. Section 5. Differential Gene Expression. Part D. Proteins. Section 6. Protein Determination and Analysis. Section 7. Sample Fractionation for Proteomics. Section 8. gel Electrophoresis. Section 9. Detection of Proteins in Gels. Section 10. Gel Profiling of Posttranslationally Modified Proteins. Section 11. Protein/Protein and Protein/Small Molecule Interactions. Section 12. Functional Proteomics. Section 13. Protein/DNA Interactions. Section 14. Protein Degradation. Section 15. Mass Spectrometry: Protein Identification and Interactions. Part E. Appendix.
Description: 1 online resource (4 volumes (xlv., 586, xlv., 444, xliii, 527, xliv., 584 pages)) : illustrations (some color).
Contents: Cover ; Contents of Volume 1; Contents of other Volumes; Contributors; Preface; PART A: CELL AND TISSUE CULTURE: ASSORTED TECHNIQUES; SECTION 1: General Techniques; CHAPTER 1: Setting up a Cell Culture Laboratory; CHAPTER 2: General Procedures for Cell Culture; CHAPTER 3: Counting Cells; CHAPTER 4: Cell Proliferation Assays: Improved Homogeneous Methods Used to Measure the Number of Cells in Culture; CHAPTER 5: Development of Serum-Free Media: Optimization of Nutrient Composition and Delivery Format; CHAPTER 6: Cell Line Authentication. CHAPTER 7: Detection of Microbial and Viral Contaminants in Cell LinesSECTION 2: Culture of Specific Cell Types: Stem Cells; CHAPTER 8: Neural Crest Stem Cells; CHAPTER 9: Postnatal Skeletal Stem Cells: Methods for Isolation and Analysis of Bone Marrow Stromal Cells from Postnatal Murine; CHAPTER 10: Establishment of Embryonic Stem Cell Lines from Adult Somatic Cells by Nuclear Transfer; CHAPTER 11: T-Cell Isolation and Propagation in vitro; CHAPTER 12: Generation of Human and Murine Dendritic Cells; SECTION 3: Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial. CHAPTER 13: Clonal Cultures in vitro for Haemopoietic Cells Using Semisolid Agar MediumCHAPTER 14: Human Skeletal Myocytes; CHAPTER 15: Growing Madin-Darby Canine Kidney Cells for Studying Epithelial Cell Biology; CHAPTER 16: Cultivation and Retroviral Infection of Human Epidermal Keratinocytes; CHAPTER 17: Three-Dimensional Cultures of Normal and Malignant Human Breast Epithelial Cells to Achieve in vivo-like Architecture and Function; CHAPTER 18: Primary Culture of Drosophila Embryo Cells; CHAPTER 19: Laboratory Cultivation of Caenorhabditis elegans and Other Free-Living Nematodes. SECTION 4: Differentiation and Reprogramming of Somatic CellsCHAPTER 20: Induction of Differentiation and Cellular Manipulation of Human Myeloid HL-60 Leukemia Cells; CHAPTER 21: Cultured PC12 Cells: A Model for Neuronal Function, Differentiation, and Survival; CHAPTER 22: Differentiation of Pancreatic Cells into Hepatocytes; CHAPTER 23: TERA2 and Its NTERA2 Subline: Pluripotent Human Embryonal Carcinoma Cells; CHAPTER 24: Embryonic Explants from Xenopus laevis as an Assay System to Study Differentiation of Multipotent Precursor Cells. CHAPTER 25: Electrofusion: Nuclear Reprogramming of Somatic Cells by Cell Hybridization with Pluripotential Stem CellsCHAPTER 26: Reprogramming Somatic Nuclei and Cells with Cell Extracts; SECTION 5: Immortalization; CHAPTER 27: Immortalization of Primary Human Cells with Telomerase; CHAPTER 28: Preparation and Immortalization of Primary Murine Cells; SECTION 6: Somatic Cell Hybrids; CHAPTER 29: Viable Hybrids between Adherent Cells: Generation, Yield Improvement, and Analysis; SECTION 7: Cell Separation Techniques; CHAPTER 30: Separation and Expansion of Human T Cells.
Series Title: Cell biology.
Responsibility: edited by Julio E. Celis.

Abstract:

This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1); Organelle and Cellular Structures, Assays (Volume 2); Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3); and, Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4). It serves as an indispensable bench companion for every life science laboratory. It provides the latest information on the plethora of technologies needed to tackle complex biological problems. It includes numerous illustrations, some in full color, supporting steps and results.

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