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Cell-cell interactions : methods and protocols

Author: Sean P Colgan
Publisher: Totowa, N.J. : Humana Press, ©2006.
Series: Methods in molecular biology (Clifton, N.J.), v. 341.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
The use of directed cell models to study the manner in which two or more cells interact has revolutionized our understanding of protein-protein interactions in a complex milieu and has provided an invaluable platform for the identification of new molecules. In Cell-Cell Interactions: Methods and Protocols, highly skilled experts in the development and utilization of cell-cell interaction assays describe in detail  Read more...
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Genre/Form: Fulltext
Illustration
Internet Resource
Electronic books
Additional Physical Format: Print version:
Cell-cell interactions.
Totowa, N.J. : Humana Press, ©2006
(DLC) 2005029552
(OCoLC)62123271
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Sean P Colgan
ISBN: 1588295230 9781588295231 9781597451130 1597451134
OCLC Number: 209947981
Description: 1 online resource (xiii, 286 pages) : illustrations (some color).
Contents: Blood-brain barrier and cell-cell interactions: methods for establishing in vitro models of the blood-brain barrier and transport measurements / Michael Aschner [and others] --
Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences / Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick. The role of junctional adhesion molecules in interactions between vascular cells / Triantafyllos Chavakis and Valeria Orlova --
Cell-cell interactions on solid matrices / Nancy A. Louis, Dionne Daniels, and Sean P. Colgan --
Cell-cell interactions in the kidney: inducible expression of mutant G protein [alpha]-subunits in Madin-Darby canine kidney cells for studies of epithelial cell junction structure and function / Ernesto Sabath and Bradley M. Denker --
Nucleotide metabolism and cell-cell interactions / Holger K. Eltzschig [and others]. Analysis of mammalian sperm-egg membrane interactions during in vitro fertilization / Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans --
Collagen gel contraction assay / Peter Ngo [and others] --
Methods to assess tissue permeability / Juan C. Ibla and Joseph Khoury --
Bacterial-bacterial cell interactions in biofilms: detection of polysaccharide intercellular adhesins by blotting and confocal microscopy / Kimberly K. Jefferson and Nuno Cerca --
A biochemical method for tracking cholera toxin transport from plasma membrane to golgi and endoplasmic reticulum / Heidi E. De Luca and Wayne I. Lencer --
Isolation and culture of murine heart and lung endothelial cells for in vitro model systems / Yaw-Chyn Lim and Frances W. Luscinskas. Expression cloning of signaling proteins regulated by cell adhesion / Michelle L. Matter and Joe W. Ramos --
Assays for the calcium sensitivity of desmosomes / Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya --
Tight junctions and cell-cell interactions / Markus Utech, Matthias Brüwer, and Asma Nusrat --
Simultaneous immunofluorescence detection of cadherin and [beta]-catenin in mouse tissue sections / Nobuko Obara. In vitro neutrophil transepithelial migration / Winston Y. Lee [and others] --
The blot rolling assay: a method for identifying adhesion molecules mediating binding under shear conditions / Robert Sackstein and Robert Fuhlbrigge --
Cell-cell interaction in the transcellular biosynthesis of novel [omega]-3-derived lipid mediators / Nan Chiang and Charles N. Serhan --
Isolation and analysis of lipid rafts in cell-cell interactions / Aimee Landry and Ramnik Xavier.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 341.
Responsibility: edited by Sean P. Colgan.
More information:

Abstract:

A collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. It features the protocols that cover a  Read more...

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