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Cell cycle oscillators : methods and protocols

Author: Amanda S Coutts; Louise Weston
Publisher: New York : Humana Press : Springer, [2016] ©2016
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 1342.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks.  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Cell Cycle Oscillators.
Humana Pr Inc 2015
(OCoLC)909327895
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Amanda S Coutts; Louise Weston
ISBN: 9781493929573 1493929577
OCLC Number: 918266261
Description: 1 online resource (xii, 352 pages) : illustrations (some color).
Contents: Cell cycle control : a system of interlinking oscillators / Randy Y.C. Poon --
Model organisms for studying the cell cycle / Zhaohua Tang --
Role of computational modeling in understanding cell sycle oscillators / Attila Csikász-Nagy and Ivan Mura --
E2F transcription factors control the roller coaster ride of cell cycle gene expression / Ingrid Thurlings and Alain de Bruin --
Cell synchronization of mouse embryonic fibroblasts / Michael J. Thwaites [and three others] --
Cell cycle synchronization in xenopus egg extracts / Peter J. Gillespie [and four others] --
Elutriation for cell cycle synchronization in fission yeast / Kazunori Kume --
Spatiotemporal investigation of phosphorylation events during cell sycle progression / Lilia Gheghiani and Olivier Gavet --
Cell cycle dynamics of proteins and post-translational modifications using quantitative immunofluorescence / Karen Akopyan, Arne Lindqvist, and Erik Müllers. Tracking the cyclin B1-GFP sensor to profile the pattern of mitosis versus mitotic bypass / Victoria Griesdoorn [and four others] --
Measuring APC/C-dependent ubiquitylation in vitro / Marc A. Jarvis [and five others] --
Using the Fly-FUCCI system for the live analysis of cell cycle dynamics in cultured drosophila cells / N. Zielke [and three others] --
Imaging cell cycle phases and transitions of living cells from yeast to woman / Hadas Segev [and four others] --
Measurement of Cdk1/Cyclin B kinase activity by specific antibodies and western blotting / Cody W. Lewis, Ryan G. Taylor, and Roy M. Golsteyn.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 1342.
Responsibility: edited by Amanda S. Coutts, Louise Weston.

Abstract:

This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.

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