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Chemical neurobiology : methods and protocols

Author: Matthew R Banghart
Publisher: New York : Humana Press, New York : Springer Science+Business Media [2013] ©2013
Series: Methods in molecular biology (Clifton, N.J.), v. 995.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in photochemistry, microscopy, and protein  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Chemical neurobiology.
[S.l.] : Humana, 2013
(OCoLC)821217443
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Matthew R Banghart
ISBN: 9781627033459 1627033459
OCLC Number: 830553847
Description: 1 online resource (xii, 219 pages) : illustrations.
Contents: Engineering k(+) channels using semisynthesis --
Chemical derivatization and purification of peptide-toxins for probing ion channel complexes --
Using yeast to study potassium channel function and interactions with small molecules --
A FLIPR assay for evaluating agonists and antagonists of GPCR heterodimers --
Characterizing caged molecules through flash photolysis and transient absorption spectroscopy --
Characterization of one- and two-photon photochemical uncaging efficiency --
Photochromic potassium channel blockers: Design and electrophysiological characterization --
A (1)h NMR assay for measuring the photostationary states of photoswitchable ligands --
Developing a photoreactive antagonist --
Development and in vitro characterization of ratiometric and intensity-based fluorescent ion sensors --
Characterization of voltage-sensitive dyes in living cells using two-photon excitation --
Characterization and validation of fluorescent receptor ligands: A case study of the ionotropic serotonin receptor --
Imaging single synaptic vesicles in mammalian central synapses with quantum dots --
Directed evolution of protein-based neurotransmitter sensors for MRI.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 995.
Responsibility: edited by Matthew R. Banghart.

Abstract:

This Methods in Molecular Biology (TM) book covers chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging  Read more...

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