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Chloroplast research in arabidopsis : methods and protocols. Volume I

Author: R Paul Jarvis
Publisher: New York : Humana Press, ©2011.
Series: Methods in molecular biology (Clifton, N.J.), v. 774.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Database:WorldCat
Summary:

Research into chloroplast biology has been hampered by heterogeneous methodologies and subject species. Now Arabidopsis has been adopted as the model research organism, and this second volume covers  Read more...

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Additional Physical Format: Online version:
Chloroplast research in arabidopsis Volume I.
New York : Humana Press, ©2011
(OCoLC)747105633
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: R Paul Jarvis
ISBN: 1617792330 9781617792335 9781617792366 1617792365
OCLC Number: 751990042
Description: x, 374 pages : illustrations ; 27 cm.
Contents: Screening or selection for chloroplast biogenesis mutants of Arabidopsis, following chemical or insertional mutagenesis / Enrique Lopez-Juez and Alison Hills --
Analysis of plastid number, size, and distribution in Arabidopsis plants by light and fluorescence microscopy / Kevin Pyke --
Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins / Stanislav Vitha and Katherine W. Osteryoung --
Transient expression and analysis of chloroplast proteins in Arabidopsis protoplasts / Dong Wook Lee and Inhwan Hwang --
Visualisation of stromules on Arabidopsis plastids / John C. Gray, James A. Sullivan, and Christine A. Newell --
Analysis of chloroplast movement and relocation in Arabidopsis / Masamitsu Wada and Sam-Geun Kong --
Studying starch content and sedimentation of amyloplast statoliths in Arabidopsis roots / John Stanga, Allison Strohm, and Patrick H. Masson --
Studying Arabidopsis chloroplast structural organisation using transmission electron microscopy / Stefan Hyman and R. Paul Jarvis --
Transplastomics in Arabidopsis : progress toward developing an efficient method / Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga --
Isolation, quantification, and analysis of chloroplast DNA / Beth A. Rowan and Arnold J. Bendich --
Measurement of transcription rates in Arabidopsis chloroplasts / Yan O. Zubo, Thomas Borner, and Karsten Liere --
Studying the structure and processing of chloroplast transcripts / Alice Barkan --
In vitro RNA-binding assay for studying trans-factors for RNA editing in chloroplasts / Toshiharu Shikanai and Kenji Okuda --
Studying translation in Arabidopsis chloroplasts / Paolo Pesaresi --
Studying proteases and protein turnover in Arabidopsis chloroplasts / Lars L.E. Sjogren and Adrian K. Clarke --
In silico methods for identifying organellar and suborganellar targeting peptides in Arabidopsis chloroplast proteins and for predicting the topology of membrane proteins / Sandra K. Tanz and Ian Small --
Rapid isolation of Arabidopsis chloroplasts and their use for in vitro protein import assays / Henrik Aronsson and R. Paul Jarvis --
Energetic manipulation of chloroplast protein import and the use of chemical cross-linkers to map protein-protein interactions / Hitoshi Inoue [and others] --
Isolation of Arabidopsis thylakoid membranes and their use for in vitro protein insertion or transport assays / Thomas Bals and Danja Schunemann --
Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction / Chiung-Chih Chu and Hsou-min Li --
Studying Arabidopsis envelope protein localization and topology using thermolysin and trypsin proteases / John Froehlich.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 774.; Springer protocols (Series)
Responsibility: edited by R. Paul Jarvis.
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