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Chromatin and chromosomal protein research : III

Author: Stein, Gary.; Stein, Janet.; Kleinsmith, Lewis J.
Publisher: New York : Academic Press, 1978.
Series: Methods in cell biology, v. 18.
Edition/Format:   Print book : EnglishView all editions and formats
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Document Type: Book
All Authors / Contributors: Stein, Gary.; Stein, Janet.; Kleinsmith, Lewis J.
ISBN: 0125641184 9780125641180
OCLC Number: 15131064
Description: xiv, 462 pages : illustrations ; 24 cm.
Contents: Part A. Chromatin fractionation. 1. Fractionation of chromatin into template-active and template-inactive portions --
2. Fractionation of chromatin by buoyant density-gradient sedimentation in metrizamide --
3. Dissection of the eukaryotic chromosome with deoxyribonucleases --
4. Isolation of amplified nucleoli from Xenopus oocytes --
5. Visualization of chromatin v-bodies --
6. Isolation and characterization of chromatin subunits --
Part B. Immunochemical analysis of chromosomal properties. 7. Serological analyses of histones --
8. Immunochemical analysis of nonhistone proteins --
9. Radioimmunoassay of nonhistone proteins --
10. Immunofluorescent techniques in the analysis of chromosomal proteins --
Part C. Sequencing of histones. 11. Peptide mapping and amino acid sequencing of histones --
12. Determination of the primary structures of histones --
Part D. Physical properties of DNA-nuclear protein complexes. 13. Preparation of chromatin and chromosomes for electron microscopy --
14. Flow-cytometric analysis of chromatin --
15. The laser microbeam as a probe for chromatin structure and function --
16. Utilization of neutron scattering for analysis of chromatin and nucleoprotein structure --
17. Circular dichroism analysis of chromatin and DNA-nuclear protein complexes --
18. Intercalating agents as probes of chromatin structure --
19. Thermal denaturation analysis of chromatin and DNA-nuclear protein complexes --
20. Thermal denaturation analysis of chromatin and DNA-nuclear protein complexes --
21. Assaying histone interactions by sedimentation equilibrium and other methods --
22. The study of histone-histone associations by chemical cross-linking.
Series Title: Methods in cell biology, v. 18.
Responsibility: edited by Gary Stein and Janet Stein, Lewis J. Kleinsmith.

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