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Chromatin immunoprecipitation assays : methods and protocols

Author: Philippe Collas
Publisher: New York, NY : Humana Press, ©2009.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 567.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Chromatin immunoprecipitation assays.
New York, NY : Humana Press, ©2009
(DLC) 2009931091
(OCoLC)310401125
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Philippe Collas
ISBN: 9781603274142 1603274146
OCLC Number: 428733172
Language Note: English.
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (x, 267 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: The state-of-the-art of chromatin immunoprecipitation / P. Collas --
Characterization and quality control of antibodies used in ChIP assays / G. Goens [and others] --
The fast chromatin immunoprecipitation method / J. Nelson, O. Denisenko and K. Bomsztyk --
[u]ChIP : chromatin immunoprecipitation for small cell numbers / J.A. Dahl and P. Collas --
Fish 'n ChIPs : Chromatin immunoprecipitation in the zebrafish embryo / L.C. Lindeman [and others] --
Epitope tagging of endogenous proteins for genome-wide chromatin immunoprecipitation analysis / Z. Wang --
Flow cytometric and laser scanning microscopic approaches in epigenetics research / L. Szekvolgyi [and others] --
Serial analysis of binding elements for transcription factors / J. Chen --
Modeling and analysis of ChIP-ChIP experiments / R. Gottardo --
Use of SNP-arrays for ChIP assays : Computational aspects / E.M. Muro [and others] --
Damid : A methylation-based chromatin profiling approach / M. Abed [and others] --
Chromosome conformation capture (from 3C to 5C) and its ChIP-based modification / A. Gavrilov [and others] --
Determining spatial chromatin organization of large genomic regions using 5C technology / N.L. Berkum and J. Dekker --
Analysis of nascent RNA transcripts by chromatin RNA immunoprecipitation / A. Obrdlik and P. Percipalle --
Methyl DNA immunoprecipitation / J.-J. Goval and J. Magdalena --
Immunoprecipitation of methylated DNA / A.L. Sørensen and P. Collas.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 567.
Responsibility: edited by Philippe Collas.

Abstract:

In this book, researchers deeply involved in the development and improvement of chromatin immunoprecipitation assays (ChIP) provide cutting-edge protocols devoted to the most recent progress in ChIP  Read more...

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From the reviews:"This is a very useful book for researchers who utilise chromatin Immunoprecipitation (ChIP). ... In conclusion, the chapters in this book describe the methods in detail and in Read more...

 
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