skip to content
Chromatin protocols Preview this item
ClosePreview this item
Checking...

Chromatin protocols

Author: Srikumar P Chellappan
Publisher: New York : Humana Press/Springer, ©2009.
Series: Methods in molecular biology (Clifton, N.J.), 523.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols,  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

 

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Aufsatzsammlung
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Srikumar P Chellappan
ISBN: 9781588298737 1588298736 9781597451901 1597451908
OCLC Number: 277069034
Description: xiii, 416 pages : illustrations.
Contents: Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos / Edgar Bonte and Peter B. Becker --
Analysis of reconstituted chromatin using a solid-phase approach / Raphael Sandaltzopoulos and Peter B. Becker --
In vivo chromatin decondensation assays : molecular genetic analysis of chromatin unfolding characteristics of selected proteins / Sherry L. Winter, Philip Wong, and Mark G. Alexandrow --
DNA methyltransferase probing of chromatin structure within populations and on single molecules / Carolina Pardo [and others] --
Visualization of the expression of HMGN nucleosomal binding proteins in the developing mouse embryo and in adult mouse tissues / Takashi Furusawa and Michael Bustin --
Drug-induced premature chromosome condensation (PCC) protocols : cytogenetic approaches in mitotic chromosome and interphase chromatin / Eisuke Gotoh --
Analysis of DNA topology in yeast chromatin / Randall H. Morse --
Preparation and analysis of uniquely positioned mononucleosomes / Daria A. Gaykalova [and others] --
Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy / Michael J. Kruhlak, Arkady Celeste, and André Nussenzweig --
Methods to study transcription-coupled repair in chromatin / Hélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera --
Cytometric analysis of DNA damage : phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs) / Toshiki Tanaka [and others] --
Detection of DNA damage induced by topoisomerase II inhibitors, gamma radiation, and crosslinking agents using the comet assay / Lori A. Hazlehurst --
Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes / Melanie Carless --
In vitro replication assay with mammalian cell extracts / Wasia Rizwani and Srikumar P. Chellappan --
Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction / Melanie Carless --
Biochemical analysis of arginine methylation in transcription / Marc Tini, Hina Naeem, and Joseph Torchia --
In vitro histone demethylase assays / Kenji Kokura and Jia Fang --
Histone chaperone as coactivator of chromatin transcription : role of acetylation / Shrikanth S. Gadad [and others] --
Histone deacetylase activity assay / Zhigang Yuan [and others] --
In vitro and in vivo assays for studying histone ubiquitination and deubiquitination / Ling Zhai, Heui-Yun Joo, and Hengbin Wang --
Detection of histone H3 phosphorylation in cultured cells and tissue sections by immunostaining / Jaya Padmanabhan --
Chromatin immunoprecipitation assays : analyzing transcription factor binding and histone modifications in vivo / Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan --
ChIP on Chip assays : genome-wide analysis of transcription factor binding and histone modifications / Smitha Pillai and Srikumar P. Chellappan --
ChIP-on-Chip analysis methods for Affymetrix tiling arrays / Sean J. Yoder and Steven A. Enkemann --
Non-radioactive assay methods for the assessment of telomerase activity and telomere length / Partha P. Banerjee and Shankar Jagadeesh --
Detecting ATM-dependent chromatin modification in DNA damage and heat shock response / Sandeep Misri, Shruti Pandita, and Tej K. Pandita.
Series Title: Methods in molecular biology (Clifton, N.J.), 523.
Responsibility: edited by Srikumar P. Chellappan.

Abstract:

Comprehensive and up-to-date, this revised second edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new  Read more...

Reviews

Editorial reviews

Publisher Synopsis

From the reviews of the second edition:"This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of Read more...

 
User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/277069034> # Chromatin protocols
    a schema:CreativeWork, schema:Book ;
    library:oclcnum "277069034" ;
    library:placeOfPublication <http://dbpedia.org/resource/New_York_City> ; # New York
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/nyu> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/364040678#Topic/eukaryontische_zelle> ; # Eukaryontische Zelle
    schema:about <http://dewey.info/class/572.87/e22/> ;
    schema:about <http://id.worldcat.org/fast/916456> ; # Eukaryotic cells
    schema:about <http://experiment.worldcat.org/entity/work/data/364040678#Topic/chromatine> ; # Chromatine
    schema:about <http://experiment.worldcat.org/entity/work/data/364040678#Topic/molekularbiologie> ; # Molekularbiologie
    schema:about <http://experiment.worldcat.org/entity/work/data/364040678#Topic/eukaryotic_cells> ; # Eukaryotic Cells
    schema:about <http://experiment.worldcat.org/entity/work/data/364040678#Topic/chromatin> ; # Chromatin
    schema:about <http://experiment.worldcat.org/entity/work/data/364040678#Topic/chromatin_ultrastructure> ; # Chromatin--ultrastructure
    schema:about <http://experiment.worldcat.org/entity/work/data/364040678#Topic/chromatin_structure> ; # Chromatin--Structure
    schema:about <http://id.loc.gov/authorities/subjects/sh85045546> ; # Eukaryotic cells
    schema:bookEdition "2nd ed." ;
    schema:bookFormat bgn:PrintBook ;
    schema:contributor <http://viaf.org/viaf/83513136> ; # Srikumar P. Chellappan
    schema:copyrightYear "2009" ;
    schema:datePublished "2009" ;
    schema:description "Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos / Edgar Bonte and Peter B. Becker -- Analysis of reconstituted chromatin using a solid-phase approach / Raphael Sandaltzopoulos and Peter B. Becker -- In vivo chromatin decondensation assays : molecular genetic analysis of chromatin unfolding characteristics of selected proteins / Sherry L. Winter, Philip Wong, and Mark G. Alexandrow -- DNA methyltransferase probing of chromatin structure within populations and on single molecules / Carolina Pardo [and others] -- Visualization of the expression of HMGN nucleosomal binding proteins in the developing mouse embryo and in adult mouse tissues / Takashi Furusawa and Michael Bustin -- Drug-induced premature chromosome condensation (PCC) protocols : cytogenetic approaches in mitotic chromosome and interphase chromatin / Eisuke Gotoh -- Analysis of DNA topology in yeast chromatin / Randall H. Morse -- Preparation and analysis of uniquely positioned mononucleosomes / Daria A. Gaykalova [and others] -- Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy / Michael J. Kruhlak, Arkady Celeste, and André Nussenzweig -- Methods to study transcription-coupled repair in chromatin / Hélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera -- Cytometric analysis of DNA damage : phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs) / Toshiki Tanaka [and others] -- Detection of DNA damage induced by topoisomerase II inhibitors, gamma radiation, and crosslinking agents using the comet assay / Lori A. Hazlehurst -- Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes / Melanie Carless -- In vitro replication assay with mammalian cell extracts / Wasia Rizwani and Srikumar P. Chellappan -- Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction / Melanie Carless -- Biochemical analysis of arginine methylation in transcription / Marc Tini, Hina Naeem, and Joseph Torchia -- In vitro histone demethylase assays / Kenji Kokura and Jia Fang -- Histone chaperone as coactivator of chromatin transcription : role of acetylation / Shrikanth S. Gadad [and others] -- Histone deacetylase activity assay / Zhigang Yuan [and others] -- In vitro and in vivo assays for studying histone ubiquitination and deubiquitination / Ling Zhai, Heui-Yun Joo, and Hengbin Wang -- Detection of histone H3 phosphorylation in cultured cells and tissue sections by immunostaining / Jaya Padmanabhan -- Chromatin immunoprecipitation assays : analyzing transcription factor binding and histone modifications in vivo / Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan -- ChIP on Chip assays : genome-wide analysis of transcription factor binding and histone modifications / Smitha Pillai and Srikumar P. Chellappan -- ChIP-on-Chip analysis methods for Affymetrix tiling arrays / Sean J. Yoder and Steven A. Enkemann -- Non-radioactive assay methods for the assessment of telomerase activity and telomere length / Partha P. Banerjee and Shankar Jagadeesh -- Detecting ATM-dependent chromatin modification in DNA damage and heat shock response / Sandeep Misri, Shruti Pandita, and Tej K. Pandita."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/364040678> ;
    schema:genre "Laboratory Manuals"@en ;
    schema:genre "Aufsatzsammlung"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://worldcat.org/issn/1064-3745> ; # Methods in molecular biology (Clifton, N.J.) ;
    schema:name "Chromatin protocols"@en ;
    schema:productID "277069034" ;
    schema:publication <http://www.worldcat.org/title/-/oclc/277069034#PublicationEvent/new_york_humana_press_springer_2009> ;
    schema:publisher <http://experiment.worldcat.org/entity/work/data/364040678#Agent/humana_press_springer> ; # Humana Press/Springer
    schema:reviews <http://www.worldcat.org/title/-/oclc/277069034#Review/-998155725> ;
    schema:url <http://www.dandelon.com/intelligentSEARCH.nsf/alldocs/DE3D11EE0F98C076C1257280001DF1D6/File/88839900.PDF?OpenElement> ;
    schema:url <http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=018675841&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA> ;
    schema:url <http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-59745-190-1> ;
    schema:url <http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018675841&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA> ;
    schema:workExample <http://worldcat.org/isbn/9781597451901> ;
    schema:workExample <http://worldcat.org/isbn/9781588298737> ;
    umbel:isLike <http://bnb.data.bl.uk/id/resource/GBA8D7288> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/277069034> ;
    .


Related Entities

<http://dbpedia.org/resource/New_York_City> # New York
    a schema:Place ;
    schema:name "New York" ;
    .

<http://experiment.worldcat.org/entity/work/data/364040678#Agent/humana_press_springer> # Humana Press/Springer
    a bgn:Agent ;
    schema:name "Humana Press/Springer" ;
    .

<http://experiment.worldcat.org/entity/work/data/364040678#Topic/chromatin_ultrastructure> # Chromatin--ultrastructure
    a schema:Intangible ;
    schema:name "Chromatin--ultrastructure"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/364040678#Topic/eukaryontische_zelle> # Eukaryontische Zelle
    a schema:Intangible ;
    schema:name "Eukaryontische Zelle"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/364040678#Topic/molekularbiologie> # Molekularbiologie
    a schema:Intangible ;
    schema:name "Molekularbiologie"@en ;
    .

<http://id.loc.gov/authorities/subjects/sh85045546> # Eukaryotic cells
    a schema:Intangible ;
    schema:name "Eukaryotic cells"@en ;
    .

<http://id.worldcat.org/fast/916456> # Eukaryotic cells
    a schema:Intangible ;
    schema:name "Eukaryotic cells"@en ;
    .

<http://viaf.org/viaf/83513136> # Srikumar P. Chellappan
    a schema:Person ;
    schema:familyName "Chellappan" ;
    schema:givenName "Srikumar P." ;
    schema:name "Srikumar P. Chellappan" ;
    .

<http://worldcat.org/isbn/9781588298737>
    a schema:ProductModel ;
    schema:isbn "1588298736" ;
    schema:isbn "9781588298737" ;
    .

<http://worldcat.org/isbn/9781597451901>
    a schema:ProductModel ;
    schema:isbn "1597451908" ;
    schema:isbn "9781597451901" ;
    .

<http://worldcat.org/issn/1064-3745> # Methods in molecular biology (Clifton, N.J.) ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/277069034> ; # Chromatin protocols
    schema:issn "1064-3745" ;
    schema:name "Methods in molecular biology (Clifton, N.J.) ;" ;
    schema:name "Methods in molecular biology," ;
    .

<http://www.worldcat.org/title/-/oclc/277069034#Review/-998155725>
    a schema:Review ;
    schema:itemReviewed <http://www.worldcat.org/oclc/277069034> ; # Chromatin protocols
    schema:reviewBody "Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function." ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.