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|Material Type:||Internet resource|
|Document Type:||Book, Internet Resource|
|All Authors / Contributors:||
Janusz A Z Jankowski; Julia M Polak; Royal Postgraduate Medical School.
|ISBN:||0521473268 9780521473262 0521478960 9780521478960|
|Description:||xviii, 459 p. : ill. ; 26 cm.|
|Contents:||Part I. Introduction, Background and Essential Theory: 1. Introduction and applications J. Jankowski - 2. History of molecular genetics R. Chinery - 3. Molecules of gene expression (protein, RNA and DNA) R. Chinery - Part II. Molecular Cell Tools: 4. Tissue analysis and morphological characterisation - 5. Cellular analysis and cell culture - 6. Chromosomal and molecular analysis - 7. Gene manipulation F. Bedford and J. Jankowski - Part III. Molecular Cell Techniques: 8. Cellular analysis - Initiation and propagation of cultured cell lines I. Freshney - Characterisation of cultured cell cines S. Kirkland and K. Henderson - Cytoxicity assays in vitro R. Phillips and B. Boullier - The production of monoclonal and polyclonal antibodies using synthetic peptides as immunogens T. RajKumar and B. Gullick - 9. Tissue analysis - Modern morphological techniques in molecular cell analysis (Immunocytochemistry autoradiography in situ hybridisation) L. Gordon and A. Bishop - An overview of methodologies for assessing proliferation and cell death R. Dover - 10. Chromosomal and molecular assays 11. Assessing proteins and nucleic acids quantitatively and qualitatively by gel fractionation and related techniques - 12. Extraction of protein, western blotting, special protein assays - RIA and ELISA - 13.Extraction of RNA and northern blot and RT PCR - 14.Extraction of mammalian DNA, PCR, Southern blot and dot blot - 15. Production of primers/probes J. Jankowski and F. Bedford - 16. Molecular cytogenetic analysis of tumours and cell lines and its application in biology and medicine - 17. Cytogenetics, FISH, allelle loss S. Jhanwar - Analysis of DNA sequence polymorphisms and screening for point mutations A. Wright and J. Prosser - 18. D. Gene manipulation - 19. Molecular level - 20. Basic cloning techniques - 21. Purification of bacterial, plasmid or phage DNA cloning in prokaryotes and construction of recombinant DNA molecules S. Legon - Creation and screening of cDNA libraries - 22. A practical approach to cloning J. Goldenring and I. Modlin - 23. Identification and isolation of novel DNA and cDna species F. Bedford and J. Jankowski - 24. Manual and automated DNA and RNA sequencing M. Jones - 25. Assessment of gene expression N. Bates and H. Hurst - 26. Cellular level - 27. Delivery of DNA into cells - 28. Production of competent cells and cell transfection S. Castledon and R. Vile - 29. Antisense RNA technology - (Oligodeoxyribonucleotides, ribozymes and retroviruses) P. L. Iverson and I. Baysever - 30. IV. Trouble-shooting: (i) Microbial contamination during cell culture K. Henderson 31.(ii) Safety aspects of genetic manipulation work S. Legon - 32. (iii) tissue storage and preparation J. Jankowski - 33. (iv) Failed experiments - general guidance J. Jankowski - 34. (v) Selection of adequate controls J. Jankowski - 35. (vi) Quality control J. Jankowski - 36. V. Gene manipulation and applications to molecular medicine: 37. Inherited genetic disease and developmental disorders P. Buss - 38. Oncology of solid tumours J. Jankowski - 39. Oncology of haematogenous cancers S. Ridge - 40. genetic therapies K. Sikora - VI. Appendices: A. Selected bibliography - B. Commercial suppliers - 41. Equipment (quantitative gel analysis and gene manipulation) - 42. Consumables - 43. Solutions.|
|Series Title:||Postgraduate medical science.|
|Responsibility:||by Janusz A.Z. Jankowski, editor ; Julia M. Polak, deputy editor ; foreword, Sir David Weatherall.|
- Genetic engineering -- Laboratory manuals.
- Molecular genetics -- Laboratory manuals.
- Molecular Biology.
- Gene Expression Regulation.
- Genetic Techniques.
- Genetic engineering -- Laboratory manuals
- Molecular genetics -- Laboratory manuals
- Gene Expression Regulation
- Genetic Techniques
- Genetics, Biochemical