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Clostridium difficile : methods and protocols

Author: Peter Mullany; Adam P Roberts
Publisher: New York : Humana Press, ©2010.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 646.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
"Clostridium difficile is a major nosocomial pathogen and has been shown to be a primary cause of antibiotic-associated disease. Recently, there has been an emergence of highly transmissible and frequently antibiotic-resistant strains, and the organism has become a considerable burden on health-care systems worldwide. At the same time, there has been a dramatic increase in our ability to study the organism. This  Read more...
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Genre/Form: Laboratory Manuals
Additional Physical Format: Online version:
Clostridium difficile.
New York : Humana Press, ©2010
(OCoLC)743289571
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Peter Mullany; Adam P Roberts
ISBN: 9781603273640 1603273646 9781603273657 1603273654
OCLC Number: 506251780
Description: x, 241 pages : illustrations.
Contents: Part I. Introduction to Clostridium Difficile and the Disease It Causes --
1. Clostridium difficile : No Longer an Enigmatic Pathogen? / Adam P. Roberts and Peter Mullany --
2. Clostridium difficile and the Disease It Causes / Torbjörn Norén --
Part II. Isolation and Culture Techniques --
3. Clostridium difficile Isolation and Culture Techniques / Mike Wren --
Part III. Methods for Typing and Epidemiological Studies --
4. Molecular Typing Methods for Clostridium difficile : Pulsed-Field GelElectrophoresis and PCR Ribotyping / Sandra Janezic and Maja Rupnik --
5. Clostridium difficile Toxinotyping / Maja Rupnik --
6. Multilocus Sequence Typing for Clostridium difficile / Ludovic Lemée and Jean-Louis Pons --
Part IV. Biochemistry of the Organism --
7. Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes / Ana Antunes and Bruno Dupuy --
8. Dissecting the Cell Surface / Robert Fagan and Neil Fairweather --
9. Human Intestinal Epithelial Response(s) to Clostridium difficile / Nazila V. Jafari, Elaine Allan, and Mona Bajaj-Elliott --
Part V. Genomics --
10. Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays / Richard Stabler, Lisa Dawson, and Brendan Wren --
Part VI. Development of Systems for Genetic Analysis of the Organism --
11. ClosTron-Targeted Mutagenesis / John T. Heap, Stephen T. Cartman, Sarah A. Kuehne, Clare Cooksley, and Nigel P. Minton --
12. Methods for Gene Cloning and Targeted Mutagenesis / Glen P. Carter, Dena Lyras, Rachael Poon, Pauline M. Howarth, and Julian I. Rood --
13. Transposon Mutagenesis in Clostridium difficile / Haitham A. Hussain, Adam P. Roberts, Rachael Whalan, and Peter Mullany --
Part VII. Animal Models of Disease --
14. Refinement of the Hamster Model of Clostridium difficile Disease / Gillian Douce and David Goulding --
15. Methods for Working with the Mouse Model / Anne Collignon.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 646.
Responsibility: edited by Peter Mullany and Adam P. Roberts.

Abstract:

This book brings together the most recently developed methods for studying clostridium difficile, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the  Read more...

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From the reviews:"This book describes a variety of research methods used to investigate the pathogenic properties of Clostridium difficile, a major nosocomial pathogen that causes a severe diarrhea Read more...

 
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