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The complement system : methods and protocols

Author: Mihaela Gadjeva
Publisher: New York : Humana Press : Springer, [2014]
Series: Methods in molecular biology (Clifton, N.J.), v. 1100.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This book presents a variety of protocols used to purify and analyze the activity of the individual complement components or pathways. It features step-by-step, readily reproducible laboratory  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Mihaela Gadjeva
ISBN: 9781627037235 1627037233
OCLC Number: 857968889
Description: xvi, 383 pages : illustrations ; 26 cm.
Contents: Overview / Mihaela Gadjeva --
Evaluation of complement function by ELISA / Anja Roos and Jörgen Wieslander --
Classical complement pathway component C1q : purification of human C1q, isolation of C1q collagen-like and globular head fragments and production of recombinant C1q-derivatives. Functional characterization / Mihaela Kojouharova --
Classical complement pathway components C1r and C1s : purification from human serum and in recombinant form and functional characterization / Véronique Rossi [and others] --
Expression and purification methods for the production of recombinant human complement component C2 / Angela W. Norton [and others] --
Purification and characterization of human and mouse complement C3 / Marieta M. Ruseva and Meike Heurich --
Purification of human complement protein C5 / Lars Sottrup-Jensen and Gregers Rom Andersen --
Quantification of complement C5b-9 binding to cells by flow cytometry / Oren Moskovich and Zvi Fishelson --
Challenges and opportunities in fractionation of recombinant human mannan-binding lectin / Thomas Vorup-Jensen --
Genotyping of FCN and MBL2 polymorphisms using pyrosequencing / Lea Munthe-Fog, Hans Ole Madsen, and Peter Garred --
Assay for estimation of the functional activity of the mannan-binding lectin pathway of the complement system / Troels R. Kjaer and Steffen Thiel --
Purification, measurement of concentration, and functional complement assay of human ficolins / Misao Matsushita [and others] --
Purification and functional analysis of human properdin / Joseph O'Flynn [and others] --
Purification and functional characterization of C4b- binding protein (C4BP) / Frida C. Mohlin and Anna M. Blom --
Purification and functional characterization of factor I / Sara C. Nilsson and Anna M. Blom --
C1 inhibitor : quantification and purification / Lilian Varga and József Dobó --
Purification, quantification, and functional analysis of complement factor H / Bing-Bin Yu [and others] --
Factor H-related proteins / Mihály Józsi and Seppo Meri --
Functional evaluation of factor H genetic and acquired abnormalities : application for atypical hemolytic uremic syndrome (aHUS) / Lubka T. Roumenina [and others] --
Anti-factor H autoantibodies assay / Marie-Agnès Dragon-Durey [and others] --
Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage / Najmunisa Karbani [and others] --
Purification of surfactant protein D (SP-D) from pooled amniotic fluid and bronchoalveolar lavage / Eswari Dodagatta-Marri [and others] --
Functional analysis of C5a effector responses in vitro and in vivo / Christian M. Karsten, Yves Laumonnier, and Jörg Köhl --
Detection of complement receptors 1 and 2 on mouse splenic B cells using flow cytometry / Luke R. Donius and John H. Weis --
Quantification of complement receptor 2 calcium signaling enhancement using flow cytometry / Luke R. Donius and John H. Weis --
Purification of C1q receptors and functional analysis / Berhane Ghebrehiwet and Ellinor I.B. Peerschke --
Detection of cell membrane-bound CD46 using flow cytometry / Martin Kolev and Claudia Kemper --
Detection of CD59 using flow cytometry / Martin Kolev --
Functional analysis of CD59 using complement-dependent cytotoxicity assay / Martin Kolev --
Detection of soluble CR3 (CD11b/CD18) by time-resolved immunofluorometry / Gitte Krogh Nielsen and Thomas Vorup-Jensen --
Analysis of the complement activation in mice / Nirmal K. Banda and Kazue Takahashi --
Opsonophagocytic assay / Markryan Dwyer and Mihaela Gadjeva.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1100.; Springer protocols (Series)
Responsibility: edited by Mihaela Gadjeva.

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