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Concepts and techniques in genomics and proteomics

Author: Nachimuthu Saraswathy; Ponnusamy Ramalingam
Publisher: Oxford ; Philadelphia : Woodhead Pub., ©2011.
Series: Woodhead Publishing series in biomedicine, no. 5.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Concepts and techniques in genomics and proteomics covers the important concepts of high-throughput modern techniques used in the genomics and proteomics field. Each technique is explained with its underlying concepts, and simple line diagrams and flow charts are included to aid understanding and memory. A summary of key points precedes each chapter within the book, followed by detailed description in the  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Saraswathy, N.
Concepts and Techniques in Genomics and Proteomics.
Burlington : Elsevier Science, ©2011
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Nachimuthu Saraswathy; Ponnusamy Ramalingam
ISBN: 9781908818058 1908818050
OCLC Number: 828743855
Notes: Originally published in 2011 by Biohealthcare Pub. (Oxford)--Ltd.
Description: 1 online resource (xxvi, 242 pages) : illustrations.
Contents: Cover; Concepts and techniques in genomics and proteomics; Copyright; Contents; List of figures; List of tables; Preface; Acknowledgements; List of abbreviations; About the authors; 1 Introduction to genes and genomes; Key concepts; 1.1 Introduction; 1.2 The cell; 1.3 Mendel's contributions; 1.4 The chromosomal theory of inheritance; 1.5 The chemical nature of genetic material; 1.6 Composition and structure of DNA; 1.7 The central dogma of life; 1.8 Genomes of prokaryotes and eukaryotes; 1.9 The molecular structure of the gene; 1.10 Conclusion; Review questions and answers. Recommended readingWeb addresses; 2 The human genome project; Key concepts; 2.1 The history of the HGP; 2.2 The budget for the HGP; 2.3 Goals of the HGP; 2.4 Laboratories and investigators involved in the HGP; 2.5 The Human Genome Organization (HUGO); 2.6 Salient findings of the HGP; 2.7 Potential applications of the HGP; 2.8 Post-HGP challenges; 2.9 Ethical, legal, social issues (ELSI) related to the HGP; 2.10 The international HapMap Project; Review questions and answers; Recommended reading; Web addresses; 3 Genomes of model organisms; Key concepts; 3.1 Introduction; 3.2 The viral genome. 3.3 Bacterial genomes3.4 Fungal genomes; 3.5 Worm genome: Caenorhabditis elegans; 3.6 Fruit fly: Drosophila melanogaster; 3.7 Plant genome; 3.8 Animal genome; 3.9 The Microbial Genome Project; Review questions and answers; Recommended reading; Web addresses; 4 High capacity vectors; Key concepts; 4.1 Introduction; 4.2 Cosmid vectors; 4.3 Fosmid vectors; 4.4 Bacteriophage P1 derived vector; 4.5 P1 derived artificial chromosome (PAC); 4.6 Bacterial artificial chromosomes (BAC); 4.7 Yeast artificial chromosome (YAC); Review questions and answers; Recommended reading; Web address. 5 DNA sequencing methodsKey concepts; 5.1 The history of DNA sequencing; 5.2 Steps in DNA sequencing; 5.3 Chemical degradation method of DNA sequencing; 5.4 The chain termination method of DNA sequencing; 5.5 Advances in DNA sequencing methods; 5.6 New sequencing methods; 5.7 Next generation sequencing methods; Review questions and answers; Recommended reading; Web addresses; 6 Genome mapping; Key concepts; 6.1 Introduction; 6.2 Importance of genome mapping in the context of genome sequencing; 6.3 Genetic mapping; 6.4 Genetic mapping in humans; 6.5 Physical mapping methods. Review questions and answersRecommended reading; Web addresses; 7 Genome sequencing methods; Key concepts; 7.1 Introduction; 7.2 The clone-by-clone genome sequencing method; 7.3 The whole genome shotgun sequencing method; 7.4 Error control in genome sequencing; Review questions and answers; Recommended reading; Web address; 8 Genome sequence assembly and annotation; Key concepts; 8.1 Introduction; 8.2 Clone contig assembly; 8.3 Genome assembly program; 8.4 Gaps and gap closing methods; 8.5 Draft and finished genome sequences; 8.6 Genome annotation; 8.7 Comparative genomics.
Series Title: Woodhead Publishing series in biomedicine, no. 5.
Responsibility: Nachimuthu Saraswathy and Ponnusamy Ramalingam.

Abstract:

Concepts and techniques in genomics and proteomics covers the important concepts of high-throughput modern techniques used in the genomics and proteomics field. Each technique is explained, with  Read more...

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